Clinical relevance of the cagA, tnpA and tnpB genes in Helicobacter pylori

Abadi, Amin Talebi Bezmin; Mobarez, Ashraf Mohhabati; Bonten, Marc J. M.; Wagenaar, Jaap A.; Kusters, Johannes G.

doi:10.1186/1471-230x-14-33

Cited by 20 publications

(16 citation statements)

References 22 publications

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“…Our finding confirmed the previous investigations which are performed in Iran (26,27). Although, there was no significant difference between CagA positive and negative H. pylori in gastric mucosa atrophy and deformation of duodenum, we have figured out that the number of CagA positives was larger than CagA negative H. pylori in gastric antrum and duodenum ulcer.…”

Section: Discussionsupporting

confidence: 92%

Helicobacter Pylori and CagA: Relationships With Esophageal and Gastroduodenal Disorders in Iranian Patients

Teymournejad¹,

Shokoohizadeh

Mobarez³

et al. 2015

Int J Enteric Pathog

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Background:The severity of Helicobacter pylori infection is associated with virulence factors of the bacteria and host immune response. H. pylori has several virulence factors which a number of them are essential to emerge clinical outcomes. Cytotoxin-associated gene A (CagA) is the most important H. pylori virulence factor. Objectives: The aim of our study was to assess a significant relationship between presence of cagA and severity of clinical manifestation in esophageal and gastroduodenal disorders. Patients and Methods: A total of 240 gastric biopsies were collected between March 2012 and August 2013 from Tehran's hospitals. Three sets of biopsy specimens were obtained from the antrum and rapid urease tests, histological examination, Polymerase Chain Reaction (PCR) assay were performed on the biopsy specimens. Results: One hundred and eight (45%) of biopsy specimens were positive with rapid urease test and ureC gene PCR. Moreover, thirty eight (35.1%) of positive specimens had cagA gene. The rate of gastric and duodenum inflammation was more in patients who carried CagA positive H. pylori strains. Whereas less inflammation and sever lesions in esophagus were found in CagA negative H. pylori strains. Conclusions: Our study demonstrates a strong relationship between CagA and esophageal and gastroduodenal disorders. The number of CagA negative H. pylori was larger than CagA positive in esophagus lesion grade A, C, and D. Therefore, cagA may have a protective effect on some esophageal diseases. In addition, the number of CagA positives was larger than CagA negative H. pylori in gastric antrum and duodenum ulcer. Thus, CagA play a role to emerge peptic and duodenal ulcers.

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Section: Discussionsupporting

confidence: 92%

Helicobacter Pylori and CagA: Relationships With Esophageal and Gastroduodenal Disorders in Iranian Patients

Teymournejad¹,

Shokoohizadeh

Mobarez³

et al. 2015

Int J Enteric Pathog

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“…In order to define a virulence factor for each bacterium, it should pass many in vivo and in vitro experiments[17-20]. However, it is worthwhile to emphasize that only a limited number of proposed virulence factors had been successfully confirmed for H. pylori [17-19]. It had been well-documented that all H. pylori strains have several virulence factors such as flagella and urease enzyme since they have a critical role in bacterial colonization[4].…”

Section: Virulence Of H Pylorimentioning

confidence: 99%

Role ofdupAin virulence ofHelicobacter pylori

Abadi¹,

Perez-Perez²

2016

WJG

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Helicobacter pylori (H. pylori) is a gastric human pathogen associated with acute and chronic gastritis, 70% of all gastric ulcers, 85% of all duodenal ulcers, and both forms of stomach cancer, mucosal-associated lymphoid tissue (MALT) lymphoma and adenocarcinoma. Recently, attention has focused on possible relationship between presence of certain virulence factor and H. pylori-associated diseases. Some contradictory data between this bacterium and related disorders has been observed since not all the colonized individuals develop to severe disease. The reported diseases plausibility related to H. pylori specific virulence factors became an interesting story about this organism. Although a number of putative virulence factors have been identified including cytotoxin-associated gene a (cagA) and vacA, there are conflicting data about their actual participation as specific risk factor for H. pylori-related diseases. Duodenal ulcer promoting gene a (dupA) is a virulence factor of H. pylori that is highly associated with duodenal ulcer development and reduced risk of gastric cancer. The prevalence of dupA in H. pylori strains isolated from western countries is relatively higher than in H. pylori strains from Asian countries. Current confusing epidemiological reports will continue unless future sophisticated and molecular studies provide data on functional and complete dupA cluster in H. pylori infected individuals. This paper elucidates available knowledge concerning role of dupA in virulence of H. pylori after a decade of its discovery.

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“…pylori, a causative agent of gastritis, duodenal ulcers, gastric ulcer and cancer [29], is an opportunistic and infectious bacterium not only in humans, but also in animals. The infection rates are over 50.8% in developing countries whereas they are more than 34.7% in developed countries [30].…”

Section: Discussionmentioning

confidence: 96%

Effect of the cagW-based gene vaccine on the immunologic properties of BALB/c mouse: an efficient candidate for Helicobacter pylori DNA vaccine

Chehelgerdi

Doosti

2020

Preprint

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Background Helicobacter pylori ( H. pylori ) infect more than half of the world population, and they cause different serious diseases such as gastric carcinomas. This study aims to design a vaccine on the basis of cagW against H. pylori infection. After pcDNA3.1 (+)- cagW -CS-NPs complex is produced, it will be administered into the muscles of healthy BALB/c mice in order to study the effect of this DNA vaccine on the interleukin status of mice, representing its effect on the immune system. After that, the results will be compared with the control groups comprising the administration of cagW -pCDNA3.1 (+) vaccine, the administration of chitosan and the administration of PBS in the muscles of mice. Methods The cagW gene of H. pylori was amplified by employing PCR, whose product was then cloned into the pcDNA3.1 (+) vector, and this cloning was confirmed by PCR and BamHI/EcoRV restriction enzyme digestion. CagW gene DNA vaccine was encapsulated in chitosan nanoparticles (pcDNA3.1 (+)- cagW -CS-NPs) using a complex co-acervation method. The stability and in vitro expression of chitosan nanoparticles were studied by DNase I digestion and transfection, and the immune responses elicited in specific pathogen-free (SPF) mice by the pcDNA3.1 (+)-cagW-CS-NPs were evaluated. Apart from that, the protective potential pcDNA3.1 (+)- cagW -CS-NPs was evaluated by challenging with H.pylori . Results The pcDNA3.1 (+)- cagW -CS-NPs comprises cagW gene of H. pylori that is encapsulated in chitosan nanoparticles, produced with good morphology, high stability, a mean diameter of 117.7 nm, and a zeta potential of + 5.64 mV. Moreover, it was confirmed that chitosan encapsulation protects the DNA plasmid from DNase I digestion, and the immunofluorescence assay showed that the cagW gene could express in HDF cells and maintain good bioactivity at the same time. In comparison to the mice immunized with the control plasmid, in vivo immunization revealed that mice immunized with pcDNA3.1 (+)- cagW -NPs showed better immune responses and prolonged release of the plasmid DNA. Conclusions This research proves chitosan-DNA nanoparticles as potent immunization candidates against H. pylori infection and paves the way for further developments in novel vaccines encapsulated in chitosan nanoparticles.

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Clinical relevance of the cagA, tnpA and tnpB genes in Helicobacter pylori

Cited by 20 publications

References 22 publications

Helicobacter Pylori and CagA: Relationships With Esophageal and Gastroduodenal Disorders in Iranian Patients

Helicobacter Pylori and CagA: Relationships With Esophageal and Gastroduodenal Disorders in Iranian Patients

Role ofdupAin virulence ofHelicobacter pylori

Effect of the cagW-based gene vaccine on the immunologic properties of BALB/c mouse: an efficient candidate for Helicobacter pylori DNA vaccine

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