Clinical Significance and Correlation of Anti‐native Dna Antibodies and Immune Complex Level in Systemic Lupus Erythematosus

Abstract

The clinical significance and correlation of anti‐native DNA antibodies and immune complex level were evaluated in the present study. Anti‐DNA antibodies were measured by radioimmunoassay using I125‐labeled DNA and immune complex was screened using the polyethylene glycol precipitation method. Anti‐DNA antibodies of higher than 40 units/ml were found exclusively in patients with systemic lupus erythematosus, especially in patients with lupus nephritis. The level of anti‐DNA antibodies was found to be directly correlated with the disease activity. Higher amounts of circulating immune complexes could be found in patients with systemic lupus erythematosus, vasculitis, and other diseases. The patients with more active lupus erythematosus also tended to have higher amounts of immune complexes, but the correlation was not so definite. There was also no reliable correlation between anti‐DNA activity and immune complex level.

Histone antibodies in systemic lupus erythematosus. A possible diagnostic tool

“…dsDNA antibodies have a lesser correlation with histone antibodies and clinical course (see Figure 2). We believe, as do many others (8,21,22) that the association of several laboratory parameters of systemic lupus erythematosus is necessary to achieve satisfactory biologic criteria of the activity of the disease. We feel that the association of our test for histone antibody, positive in 87% of patients with active disease, together with the various other measurements recognized as useful (circulating immune complexes, dsDNA antibodies, cryoglobulins, complement levels) might assist in the better surveillance of lupus patients.…”

Humoral Immunity in Patients With Tinea Versicolor

“…Determination of circulating immune complexes (CIC) with 3% polyethylene glycol precipitation (PEG-ppt) method: The method of precipitation with 3% polyethylene glycol (PEG) (25) was used in this study. Earlier studies have shown that 20 % PEG can precipitate most of the immunoglobulins, but not the other smaller molecule proteins.…”