Clinical Significance of Glycoprotein Non-metastatic B and Its Association with EGFR/HER2 in Gastrointestinal Cancer

Tajima, Jesse Yu; Futamura, Manabu; Gaowa, Siqin; Mori, Rintaro; Tanahashi, Takao; Tanaka, Yoshihiro; Matsuhashi, Nobuhisa; Takahashi, Tsuyoshi; Yamaguchi, Kazuya; Miyazaki, Tatsuhiko; Yoshida, Kazuhiro

doi:10.7150/jca.20266

Cited by 6 publications

(6 citation statements)

References 40 publications

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“…Transmembrane glycoprotein NMB (GPNMB) was detected in stage 3 breast cancer tissues. This finding supports Tajima et al [95], Maric et al [96], and Rose et al [97], among other studies with similar findings in malignancies including melanoma, glioma, breast cancer, and gastric cancer. On a physiological level, GPNMB improves cell invasion and motility, enabling metastasis.…”

Section: Discussionsupporting

confidence: 89%

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Comparative proteomic analysis of different stages of breast cancer tissues using ultra high performance liquid chromatography tandem mass spectrometer

et al. 2020

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BackgroundBreast cancer is the fifth most prevalent cause of death among women worldwide. It is also one of the most common types of cancer among Malaysian women. This study aimed to characterize and differentiate the proteomics profiles of different stages of breast cancer and its matched adjacent normal tissues in Malaysian breast cancer patients. Also, this study aimed to construct a pertinent protein pathway involved in each stage of cancer. MethodsIn total, 80 samples of tumor and matched adjacent normal tissues were collected from breast cancer patients at Seberang Jaya Hospital (SJH) and Kepala Batas Hospital (KBH), both in Penang, Malaysia. The protein expression profiles of breast cancer and normal tissues were mapped by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The Gel-Eluted Liquid Fractionation Entrapment Electrophoresis (GEL-FREE) Technology System was used for the separation and fractionation of extracted proteins, which also were analyzed to maximize protein detection. The protein fractions were then analyzed by tandem mass spectrometry (LC-MS/MS) analysis using LC/MS LTQ-Orbitrap Fusion and Elite. This study identified the proteins contained within the tissue samples using de novo sequencing and database matching via PEAKS software. We performed two different pathway analyses, DAVID and STRING, in the sets of proteins from stage 2 and stage 3 breast cancer samples. The lists of molecules were generated by the REACTOME-FI plugin, part of the CYTOSCAPE tool, and linker nodes were added in order to generate a connected network. Then, pathway enrichment was obtained, and a graphical model was created to depict the participation of the input proteins as well as the linker nodes.

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Section: Discussionsupporting

confidence: 89%

“…On a physiological level, GPNMB improves cell invasion and motility, enabling metastasis. It is therefore logical that, according to Tajima et al [95], a high level of GPNMB expression indicates a worse prognosis. This means that GPNMB should be recognized as an important candidate for targeted therapy of malignancies [96].…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomic analysis of different stages of breast cancer tissues using ultra high performance liquid chromatography tandem mass spectrometer

et al. 2020

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“…A variety of signal transduction pathways are involved in the downstream biological effects activated by EGFR, such as RAS-RAF-MAPK, PI3K-PTEN-AKT, and JAK/STAT. Most of the above signal pathways are also involved in antibodymediated resistance to EGFR inhibition machine [17][18][19][20][21]. Any gene changes in the signaling pathway, such as KRAS, NRAS, BRAF, and PIK3CA gene mutations, may lead to the constitutive activation of EGFR and subsequent changes in intracellular signaling to eventually achieve drug resistance [22,23].…”

Section: Discussionmentioning

confidence: 99%

FOXD1 Regulates the Sensitivity of Cetuximab by Regulating the Expression of EGFR in Head and Neck Squamous Cell Cancer

Zhang

et al. 2022

Journal of Healthcare Engineering

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Objectives. Previous experiments have shown that growth factor receptors play important role in tumor proliferation, metastasis, and therapeutic effect of chemotherapeutic drugs. At the same time, forkhead box D1 (FOXD1) plays an important role in a variety of signal transmission, but its expression profile was known little about head and neck cancer. The purpose of this experiment was to explore the regulation of FOXD1 on the tumor progression of head and neck cancer and to explore the correlation of FOXD1 on the expression of growth factor receptors (EGFR). Methods. The bioinformatics online database analyzed the expression of FOXD1 and EGFR in tumor tissues and nontumor tissues. Real-time quantitative PCR was used to detect the FOXD1 and EGFR expression in 45 tumor tissues and 15 nontumor tissues. The plasmid was used to construct FOXD1 overexpressing head and neck squamous cell cancer lines and observe the clonal formation and invasion of tumor cells under the intervention of EGFR-specific antibody—cetuximab. Results. The expression of FOXD1 and EGFR in tumor tissues was higher than that in nontumor tissues. The higher expression of FOXD1 and EGFR was not conducive to the prognosis of patients. The expression of FOXD1 and EGFR was positively correlated, and immunohistochemical analysis showed the high expression of FOXD1 and EGFR has close relation to the advanced stage of the tumor. In vitro cell experiments proved that overexpression of FOXD1 can partially offset the cloning ability of cetuximab on head and neck tumor cells. Conclusion. FOXD1 has an important regulatory role in the progression of head and neck cancer, and its abnormally high expression was not conducive to the prognosis of cancer patients. FOXD1 can regulate the expression of growth factor receptors in head and neck cancer, which provides a new idea for the better use of tumor growth factor receptor-specific antibodies for collaborative therapy.

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“…These data suggest that NSBP1 plays an oncogenic role in gastric cancer. Notably, recent studies have shown that lentivirus mediated shRNA against genes with potential oncogenic role provides a new avenue for developing effective therapy for a variety of cancers 16-20. Therefore, it is promising to develop lentivirus mediated NSBP1 shRNA as a novel therapeutic approach for gastric cancer.…”

Section: Discussionmentioning

confidence: 99%

The nucleosome binding protein 1 promotes the growth of gastric cancer cells

et al. 2019

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Nucleosome binding protein 1 (NSBP1) is identified as a new member of HMGN family and is abnormally overexpressed in a variety of tumors. However, it remains unclear whether NSBP1 is overexpressed and promotes gastric cancer. In this study we employed RNAi mediated knockdown of NSBP1 to investigate potential oncogenic role of NSBP1 in gastric cancer. In BGC823 and SGC7901 gastric cancer cell lines, we showed that NSBP1 knockdown decreased cell proliferation while increased apoptosis in vitro. Western blot analysis showed that NSBP1 knockdown decreased the levels of anti-apoptotic protein Bcl-2 while increased the levels of pro-apoptotic protein Bax. In addition, NSBP1 knockdown inhibited the growth and increased the apoptosis of SGC7901 cells xenografted in nude mice. In conclusion, this study provides the first evidence that NSBP1 enhances the proliferation while inhibits the apoptosis of gastric cancer cells, and this is related to the regulation of the expression of apoptosis related proteins by NSBP1. These data suggest that NSBP1 plays oncogenic role in gastric cancer.

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Clinical Significance of Glycoprotein Non-metastatic B and Its Association with EGFR/HER2 in Gastrointestinal Cancer

Cited by 6 publications

References 40 publications

Comparative proteomic analysis of different stages of breast cancer tissues using ultra high performance liquid chromatography tandem mass spectrometer

Comparative proteomic analysis of different stages of breast cancer tissues using ultra high performance liquid chromatography tandem mass spectrometer

FOXD1 Regulates the Sensitivity of Cetuximab by Regulating the Expression of EGFR in Head and Neck Squamous Cell Cancer

The nucleosome binding protein 1 promotes the growth of gastric cancer cells

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