Clinical significance of HAX-1 expression in laryngeal carcinoma

You, Yiwen; Yao, Hao; You, Bo; Li, Xingyu; Ni, Haosheng; Shi, Si; Shan, Ying; Cao, Xiaolei

doi:10.1016/j.anl.2014.12.003

Cited by 13 publications

(14 citation statements)

References 27 publications

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“…Immunohistochemistry and Western blot analysis were carried out according to the previous report . For assessment of ADAM10, the staining intensity and relative percentage of immunostained cells were analyzed and evaluated by two pathologists blinded to the data.…”

Section: Methodsmentioning

confidence: 99%

Effects of ADAM10 upregulation on progression, migration, and prognosis of nasopharyngeal carcinoma

et al. 2015

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A disintegrin and metalloprotease 10 (ADAM10) is a typical member of the ADAMs family, which has been reported to be upregulated in various types of cancers and contribute to cancer progression and metastasis. However, little is known about the role of ADAM10 in nasopharyngeal carcinoma (NPC). The purpose of this study is to explore ADAM10 expression status and its biological functions in NPC. We first examined the expression of ADAM10 in NPC tissues and cell lines by immunohistochemistry, Western blotting, PCR, and immunofluorescence analysis. We observed that ADAM10 was significantly elevated in NPC and its expression level was correlated with T classification (P = 0.044), distant metastasis (P = 0.016), TNM clinical stage (P = 0.013), and proliferation marker Ki‐67 expression (P = 0.001). Patients with NPC with high expression of ADAM10 had shorter overall survival rates. In addition, knockdown of ADAM10 by RNAi was found to inhibit the CNE‐2 cell proliferation and migration. Our findings hinted that overexpression of ADAM10 promotes the progression and migration of NPC, which makes it a potential therapeutic target for the treatment of tumor metastases in NPC.

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Section: Methodsmentioning

confidence: 99%

Effects of ADAM10 upregulation on progression, migration, and prognosis of nasopharyngeal carcinoma

et al. 2015

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“…Immunohistochemical staining and Western blot analysis was performed as described [18]. The antibodies used in IHC included: anti-human CD93 polyclonal antibody (diluted 1:40; R&D Systems) and anti-CD31 (diluted 1:50; Abcam).…”

Section: Immunohistochemistry (Ihc) and Western Blot Analysismentioning

confidence: 99%

Elevated expression of CD93 promotes angiogenesis and tumor growth in nasopharyngeal carcinoma

Bao

Tang

Zhang

et al. 2016

Biochemical and Biophysical Research Communications

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“…IHC analysis was carried out as previously reported 25,26. The primary antibody used was ab22857 against Foxj2 (1:50; Santa Cruz Biotechnology Inc, Dallas, TX, USA) and slides were incubated overnight at 4°C.…”

Section: Ihc Stainingmentioning

confidence: 99%

“…Western blot analysis was performed as described previously 25,26. The primary antibodies used for Western blot analysis were as follows: Foxj2 polyclonal antibody (1:1,000; Santa Cruz Biotechnology) and β-actin polyclonal antibody (1:2,000; Santa Cruz Biotechnology).…”

Section: Ihc Stainingmentioning

confidence: 99%

<em>Foxj2</em> overexpression is associated with poor prognosis, progression, and metastasis in nasopharyngeal carcinoma

Shan

Chang

Shi

et al. 2017

OTT

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Foxj2, a novel member of Forkhead box family, has been reported to play an important role in tumorigenesis, progression, and metastasis of certain cancers. However, the expression status and effects of Foxj2 on nasopharyngeal carcinoma (NPC) progression and metastasis remain debated. In this study, we first examined the expression of Foxj2 in NPC by immunohistochemistry and Western blotting analysis. We confirmed significantly elevated expression of Foxj2 in NPC tissues and cell lines. Next, the relationships between Foxj2 expression levels and the clinicopathological factors were investigated. Its expression level correlated with T-classification (P=0.026), distant metastasis (P=0.004), and clinical stage (P=0.029). In addition, high expression of Foxj2 was associated with poor prognosis in NPC patients. The effects of Foxj2 on cell proliferation and migration were explored by RNA interference (RNAi) with CCK-8 assay, cell cycle analyses, wound healing, and transwell assay. In conclusion, our data indicate that Foxj2 upregulation promotes the progression and migration of NPC. It makes Foxj2 serve as a potential therapeutic target for the treatment of NPC.

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Clinical significance of HAX-1 expression in laryngeal carcinoma

Cited by 13 publications

References 27 publications

Effects of ADAM10 upregulation on progression, migration, and prognosis of nasopharyngeal carcinoma

Effects of ADAM10 upregulation on progression, migration, and prognosis of nasopharyngeal carcinoma

Elevated expression of CD93 promotes angiogenesis and tumor growth in nasopharyngeal carcinoma

<em>Foxj2</em> overexpression is associated with poor prognosis, progression, and metastasis in nasopharyngeal carcinoma

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