Clinical significance of TT virus (TTV) infection in chronic hepatitis C patients with high dose interferon-alpha therapy in Taiwan: re-evaluated by using new set of TTV primers

Dai, Chia Yen; Yu, Ming; Lin, Zu Yau; Chen, Shinn Cherng; Hsieh, Ming Yen; Lee, Li Po; Hou, Nei Jen; Hsieh, Ming Yuh; Wang, Liang Yen; Tsai, Juei‐Hsiung; Chuang, Wan‐Long; Chang, Wei‐An

doi:10.1016/s1386-6346(03)00231-6

Cited by 4 publications

(1 citation statement)

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“…By using the single step PCR method that targets 5'-UTR , a surprising increase in prevalence, from 23% to 92% was reported for blood donors in Japan [16] and from 11% to 51% for HCV infected subjects in Taiwan [21]. 49.5% positivity among blood donors could be detected in France with this set [20].…”

Section: Discussionmentioning

confidence: 99%

Performance of three PCR methods targeting different regions of viral genome for the detection of TTV in Non A-E hepatitis, chronic B and C hepatitis and healthy blood donors

et al. 2006

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AbstractTT virus (TTV) was suggested to be the etiologic agent for non A-E hepatitis but this could not yet be proven due to high detection rates not only in hepatitis but also in healthy persons and sensitivity differences of PCR methods employed. The aim of this study was to evaluate TTV DNA positivity in non A-E hepatitis cases, chronic HBV and HCV hepatitis cases and healthy blood donors via PCR systems that target all regions of the viral genome used for viral detection. 23 non A-E hepatitis, 28 chronic HCV, 21 chronic HBV cases and 56 healthy blood donors were included in the study and evaluated by PCR protocols that target 5′-UTR, 3′-UTR and N22 (ORF1) regions. As a result, 3′-UTR and 5′-UTR PCR had comparable detection rates that were higher than N22 PCR. Differences in detection rates among study groups were not statistically significant for any PCR method. Hepatic enzyme levels of the patients were not correlated with the presence of TTV DNA. Detection rate was significantly higher for Non A-E hepatitis group when positivity rates from all methods were combined. These results suggest an alteration of viral genotypes in Non A-E hepatitis which might be associated with pathogenesis.

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Section: Discussionmentioning

confidence: 99%