1989
DOI: 10.1507/endocrine1927.65.8_807
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Clinical Studies Using a Highly Sensitive Radioimmunoassay for Mid-Region and Carboxy Terminus of Parathyroid Hormone in Normal, Hypo-and Hypercalcemic States

Abstract: Parathyroid hormone radioimmunoassay (RIA), specific for mid-region of the PTH molecule, has been proven to be extremely useful for the differential diagnosis of abnormal calcium metabolism. Recently, we developed a highly sensitive RIA for PTH, consisting of PTH antiserum (CH9), 125I labelled Tyr42 hPTH (43-68) and synthetic hPTH (1-84) as standard. This RIA cross-reacted with mid-region and carboxyl terminals of PTH. The within-assay and between-assay coefficients of variation were less than 4.6% and less th… Show more

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Cited by 28 publications
(11 citation statements)
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“…The data were adjusted with urinary creatinine concentration for the exception of aberration which was induced by urine volume. In addition to these markers, measurements were taken of serum calcium (Ca) by the orthocresolphthalein complexone (OCPC) method [17], serum phosphorus (P) by direct determination of molybdic acid [18], serum alkaline phosphatase (AlP) by the paranitrophenol (PNP) substrate method [19], and the serum level of high sensitive parathyroid hormone (PTH) by RIA [20]. The range of measurement in healthy adults were serum Oc 3.1-12.7 ng/ml; urinary Dpd 2.1-5.4 nMol/liter ⅐ Cr; serum Ca 4.1-5.0 mEq/liter; serum P 2.5-4.5 mg/dl; serum AlP 74-223 U/liter; and serum PTH 74-273 pg/ml.…”
Section: Measurement Of Biochemical Markers (Bm) For Bone Metabolismmentioning
confidence: 99%
“…The data were adjusted with urinary creatinine concentration for the exception of aberration which was induced by urine volume. In addition to these markers, measurements were taken of serum calcium (Ca) by the orthocresolphthalein complexone (OCPC) method [17], serum phosphorus (P) by direct determination of molybdic acid [18], serum alkaline phosphatase (AlP) by the paranitrophenol (PNP) substrate method [19], and the serum level of high sensitive parathyroid hormone (PTH) by RIA [20]. The range of measurement in healthy adults were serum Oc 3.1-12.7 ng/ml; urinary Dpd 2.1-5.4 nMol/liter ⅐ Cr; serum Ca 4.1-5.0 mEq/liter; serum P 2.5-4.5 mg/dl; serum AlP 74-223 U/liter; and serum PTH 74-273 pg/ml.…”
Section: Measurement Of Biochemical Markers (Bm) For Bone Metabolismmentioning
confidence: 99%
“…The coefficient of variation for tHcy was 2.26% for the within-assay precision (n=7) and 2.96% for the between-assay precision (n=10). The same blood and serum samples from all dialyzed subjects were used to monitor other risk factors for vascular disease, including concentrations of total and high-density lipoprotein cholesterol (HDL-C), triglyceride, lipoprotein(a), uric acid, calcium, inorganic phosphate, and parathyroid hormone (Yamasa method 14 ).…”
Section: Biochemical Analysismentioning
confidence: 99%
“…The calcium, phosphate, creatinine, and highly sensitive plasma PTH (HS-PTH) levels were determined from the serum samples. The HS-PTH level was measured by the double antibody method (PTH kit, Yamasa Shoyu Co., Choshi, Chiba, Japan) [10,12].…”
Section: Eh Testmentioning
confidence: 99%