Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA

Patchsung, Maturada; Jantarug, Krittapas; Pattama, Archiraya; Aphicho, Kanokpol; Suraritdechachai, Surased; Meesawat, Piyachat; Sappakhaw, Khomkrit; Leelahakorn, Nattawat; Ruenkam, Theerawat; Wongsatit, Thanakrit; Athipanyasilp, Niracha; Eiamthong, Bhumrapee; Lakkanasirorat, Benya; Phoodokmai, Thitima; Niljianskul, Nootaree; Pakotiprapha, Danaya; Chanarat, Sittinan; Homchan, Aimorn; Tinikul, Ruchanok; Kamutira, Philaiwarong; Phiwkaow, Kochakorn; Soithongcharoen, Sahachat; Kantiwiriyawanitch, Chadaporn; Pongsupasa, Vinutsada; Trisrivirat, Duangthip; Jaroensuk, Juthamas; Wongnate, Thanyaporn; Maenpuen, Somchart; Chaiyen, Pimchai; Kamnerdnakta, Sirichai; Swangsri, Jirawat; Chuthapisith, Suebwong; Sirivatanauksorn, Yongyut; Chaimayo, Chutikarn; Sutthent, Ruengpung; Kantakamalakul, Wannee; Joung, Julia; Ladha, Alim; Jin, Xin; Gootenberg, Jonathan S.; Abudayyeh, Omar O.; Zhang, Feng; Horthongkham, Navin; Uttamapinant, Chayasith

doi:10.1038/s41551-020-00603-x

Cited by 518 publications

(566 citation statements)

References 32 publications

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“…If multiple studies were conducted on the same set of patient samples, only a study with the highest sensitivity and specificity was used. For example, Patchsung et al 67 reported two CRISPR diagnosis studies on the same set of patient samples, one using fluorescent readout and the other using lateral flow assay. In our analysis, we included only the fluorescent readout study, which had higher sensitivity and similar specificity to the lateral flow assay.…”

Section: Publication Bias Publication Bias Of All 81 Included Studiementioning

confidence: 99%

The diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: A systematic review and meta-analysis

Subsoontorn

Lohitnavy

Kongkaew

2020

Sci Rep

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Many recent studies reported coronavirus point-of-care tests (POCTs) based on isothermal amplification. However, the performances of these tests have not been systematically evaluated. Cochrane Handbook for Systematic Reviews of Diagnostic Test Accuracy was used as a guideline for conducting this systematic review. We searched peer-reviewed and preprint articles in PubMed, BioRxiv and MedRxiv up to 28 September 2020 to identify studies that provide data to calculate sensitivity, specificity and diagnostic odds ratio (DOR). Quality Assessment of Diagnostic Accuracy Studies 2 (QUADAS-2) was applied for assessing quality of included studies and Preferred Reporting Items for a Systematic Review and Meta-analysis of Diagnostic Test Accuracy Studies (PRISMA-DTA) was followed for reporting. We included 81 studies from 65 research articles on POCTs of SARS, MERS and COVID-19. Most studies had high risk of patient selection and index test bias but low risk in other domains. Diagnostic specificities were high (> 0.95) for included studies while sensitivities varied depending on type of assays and sample used. Most studies (n = 51) used reverse transcription loop-mediated isothermal amplification (RT-LAMP) to diagnose coronaviruses. RT-LAMP of RNA purified from COVID-19 patient samples had pooled sensitivity at 0.94 (95% CI: 0.90–0.96). RT-LAMP of crude samples had substantially lower sensitivity at 0.78 (95% CI: 0.65–0.87). Abbott ID Now performance was similar to RT-LAMP of crude samples. Diagnostic performances by CRISPR and RT-LAMP on purified RNA were similar. Other diagnostic platforms including RT- recombinase assisted amplification (RT-RAA) and SAMBA-II also offered high sensitivity (> 0.95). Future studies should focus on the use of un-bias patient cohorts, double-blinded index test and detection assays that do not require RNA extraction.

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Section: Publication Bias Publication Bias Of All 81 Included Studiementioning

confidence: 99%

The diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: A systematic review and meta-analysis

Subsoontorn

Lohitnavy

Kongkaew

2020

Sci Rep

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“…Notably, target genes in buccal samples can be detected electrically via inactivated Cas9 immobilized on a graphene field-effect transistor without the need for amplification 2 , and Cas13 has been used to monitor opportunistic viral infections in blood and urine samples following organ transplantation, and to detect transplant rejection in urine samples 3 . And, most recently, fast and inexpensive lateral-flow fluorescence assays containing Cas12a or Cas13a can detect SARS-CoV-2 RNA accurately in nasopharyngeal samples [4][5][6] .…”

mentioning

confidence: 99%

Henceforth CRISPR

2020

Nat Biomed Eng

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“…Comparison with RT-PCR results showed that both were in complete agreement. 70 A simple and rapid assay called the “all in one dual CRISPR-Cas12a” has recently been developed for the detection of SARS-CoV-2 with high sensitivity and high detection specificity. It can be carried out as a single tube reaction and provides visual detection for which it could be used as a point-of-care test.…”

Section: Nanoarchitectronics In Diagnosis and Detection Of Covid-19mentioning

confidence: 99%

Architectured Therapeutic and Diagnostic Nanoplatforms for Combating SARS-CoV-2: Role of Inorganic, Organic, and Radioactive Materials

Pandey

Nikam

Mutalik

et al. 2020

ACS Biomater. Sci. Eng.

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Although extensive research is being done to combat SARS-CoV-2, we are yet far away from a robust conclusion or strategy. With an increased amount of vaccine research, nanotechnology has found its way into vaccine technology. Researchers have explored the use of various nanostructures for delivering the vaccines for enhanced efficacy. Apart from acting as delivery platforms, multiple studies have shown the application of inorganic nanoparticles in suppressing the growth as well as transmission of the virus. The present review gives a detailed description of various inorganic nanomaterials which are being explored for combating SARS-CoV-2 along with their role in suppressing the transmission of the virus either through air or by contact with inanimate surfaces. The review further discusses the use of nanoparticles for development of an antiviral coating that may decrease adhesion of SARS-CoV-2. A separate section has been included describing the role of nanostructures in biosensing and diagnosis of SARS-CoV-2. The role of nanotechnology in providing an alternative therapeutic platform along with the role of radionuclides in SARS-CoV-2 has been described briefly. Based on ongoing research and commercialization of this nanoplatform for a viral disease, the nanomaterials show the potential in therapy, biosensing, and diagnosis of SARS-CoV-2.

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Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA

Cited by 518 publications

References 32 publications

The diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: A systematic review and meta-analysis

The diagnostic accuracy of isothermal nucleic acid point-of-care tests for human coronaviruses: A systematic review and meta-analysis

Henceforth CRISPR

Architectured Therapeutic and Diagnostic Nanoplatforms for Combating SARS-CoV-2: Role of Inorganic, Organic, and Radioactive Materials

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