Clinical validation of SERS metasurface SARS-CoV-2 biosensor

Bilgin, Buse; Torun, Hulya; İlgü, Müslüm; Yanik, Cenk; Batur, Sükrü Numan; Çelik, Süleyman Utku; Ozturk, Meric; Doğan, Özlem; Ergönül, Önder; Solaroğlu, İhsan; Can, Füsun; Onbaşlı, Mehmet C.

doi:10.1117/12.2607929

Cited by 3 publications

(8 citation statements)

References 13 publications

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“…Recognizing molecules, like antibodies or aptamers, can be used to provide the specificity of virus detection along with an increase in the limit of detection [ 11 , 12 , 13 ]. The most common approach is to decorate a SERS-active surface with recognizing molecules enhancing the local concentration of the target of interest near the surface [ 15 , 20 , 21 , 22 , 23 ]. A complementary approach uses labeled recognizing molecules to trace the spectrum of the artificial molecule that is detectable in the nanomolar range of concentrations [ 22 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…The most common approach is to decorate a SERS-active surface with recognizing molecules enhancing the local concentration of the target of interest near the surface [ 15 , 20 , 21 , 22 , 23 ]. A complementary approach uses labeled recognizing molecules to trace the spectrum of the artificial molecule that is detectable in the nanomolar range of concentrations [ 22 , 23 , 24 , 25 ]. The typical Raman active labels used for virus detection are fluorescent dyes like Cyanine 3 (Cy3), Cyanine 5.5 (Cy5.5), Bodipy FL (BDP FL), and Rhodamine Red-X (RRX) [ 22 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…A complementary approach uses labeled recognizing molecules to trace the spectrum of the artificial molecule that is detectable in the nanomolar range of concentrations [ 22 , 23 , 24 , 25 ]. The typical Raman active labels used for virus detection are fluorescent dyes like Cyanine 3 (Cy3), Cyanine 5.5 (Cy5.5), Bodipy FL (BDP FL), and Rhodamine Red-X (RRX) [ 22 , 23 , 24 , 25 ]. Labeled recognizing molecules are either approaching the surface due to sandwich-like ternary complexes [ 22 , 23 ] or retiring from the surface due to binding with viral particles [ 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…The typical Raman active labels used for virus detection are fluorescent dyes like Cyanine 3 (Cy3), Cyanine 5.5 (Cy5.5), Bodipy FL (BDP FL), and Rhodamine Red-X (RRX) [ 22 , 23 , 24 , 25 ]. Labeled recognizing molecules are either approaching the surface due to sandwich-like ternary complexes [ 22 , 23 ] or retiring from the surface due to binding with viral particles [ 24 , 25 ]. In both cases, the Q-factor and stability in biological fluids are the key parameters that are to be considered.…”

Section: Introductionmentioning

confidence: 99%

“…In both cases, the Q-factor and stability in biological fluids are the key parameters that are to be considered. Silver nanoislands and lithography-based substrates were used for the sandwich-like complex aptamer1-virus-aptamer2 [ 22 , 23 ]. The first one provided nice SERS spectra for ternary complexes with Cy3 and BDP FL-labeled aptamers [ 22 ]; however, the stability of the nanostructured surface in biological fluids was revealed to be suboptimal (authors’ unpublished data).…”

Section: Introductionmentioning

confidence: 99%

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Model of the SARS-CoV-2 Virus for Development of a DNA-Modified, Surface-Enhanced Raman Spectroscopy Sensor with a Novel Hybrid Plasmonic Platform in Sandwich Mode

et al. 2022

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The recent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has posed a great challenge for the development of ultra-fast methods for virus identification based on sensor principles. We created a structure modeling surface and size of the SARS-CoV-2 virus and used it in comparison with the standard antigen SARS-CoV-2—the receptor-binding domain (RBD) of the S-protein of the envelope of the SARS-CoV-2 virus from the Wuhan strain—for the development of detection of coronaviruses using a DNA-modified, surface-enhanced Raman scattering (SERS)-based aptasensor in sandwich mode: a primary aptamer attached to the plasmonic surface—RBD-covered Ag nanoparticle—the Cy3-labeled secondary aptamer. Fabricated novel hybrid plasmonic structures based on “Ag mirror-SiO2-nanostructured Ag” demonstrate sensitivity for the detection of investigated analytes due to the combination of localized surface plasmons in nanostructured silver surface and the gap surface plasmons in a thin dielectric layer of SiO2 between silver layers. A specific SERS signal has been obtained from SERS-active compounds with RBD-specific DNA aptamers that selectively bind to the S protein of synthetic virion (dissociation constants of DNA-aptamer complexes with protein in the range of 10 nM). The purpose of the study is to systematically analyze the combination of components in an aptamer-based sandwich system. A developed virus size simulating silver particles adsorbed on an aptamer-coated sensor provided a signal different from free RBD. The data obtained are consistent with the theory of signal amplification depending on the distance of the active compound from the amplifying surface and the nature of such a compound. The ability to detect the target virus due to specific interaction with such DNA is quantitatively controlled by the degree of the quenching SERS signal from the labeled compound. Developed indicator sandwich-type systems demonstrate high stability. Such a platform does not require special permissions to work with viruses. Therefore, our approach creates the promising basis for fostering the practical application of ultra-fast, amplification-free methods for detecting coronaviruses based on SARS-CoV-2.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Model of the SARS-CoV-2 Virus for Development of a DNA-Modified, Surface-Enhanced Raman Spectroscopy Sensor with a Novel Hybrid Plasmonic Platform in Sandwich Mode

et al. 2022

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Clinical validation of SARS-CoV-2 electrochemical immunosensor based on the spike-ACE2 complex

Vásquez,

Orozco

2024

Journal of Virological Methods

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Aptamer-coated track-etched membranes with a nanostructured silver layer for single virus detection in biological fluids

Кукушкин

Kristavchuk

Andreev

et al. 2023

Front. Bioeng. Biotechnol.

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Aptasensors based on surface-enhanced Raman spectroscopy (SERS) are of high interest due to the superior specificity and low limit of detection. It is possible to produce stable and cheap SERS-active substrates and portable equipment meeting the requirements of point-of-care devices. Here we combine the membrane filtration and SERS-active substrate in the one pot. This approach allows efficient adsorption of the viruses from the solution onto aptamer-covered silver nanoparticles. Specific determination of the viruses was provided by the aptamer to influenza A virus labeled with the Raman-active label. The SERS-signal from the label was decreased with a descending concentration of the target virus. Even several virus particles in the sample provided an increase in SERS-spectra intensity, requiring only a few minutes for the interaction between the aptamer and the virus. The limit of detection of the aptasensor was as low as 10 viral particles per mL (VP/mL) of influenza A virus or 2 VP/mL per probe. This value overcomes the limit of detection of PCR techniques (∼103 VP/mL). The proposed biosensor is very convenient for point-of-care applications.

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Clinical validation of SERS metasurface SARS-CoV-2 biosensor

Cited by 3 publications

References 13 publications

Model of the SARS-CoV-2 Virus for Development of a DNA-Modified, Surface-Enhanced Raman Spectroscopy Sensor with a Novel Hybrid Plasmonic Platform in Sandwich Mode

Model of the SARS-CoV-2 Virus for Development of a DNA-Modified, Surface-Enhanced Raman Spectroscopy Sensor with a Novel Hybrid Plasmonic Platform in Sandwich Mode

Clinical validation of SARS-CoV-2 electrochemical immunosensor based on the spike-ACE2 complex

Aptamer-coated track-etched membranes with a nanostructured silver layer for single virus detection in biological fluids

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