Clinical value of DNA image cytometry in effusions with atypia

Thunnissen, Erik; Buchholtz, R. T. F.; Woutersen, D.; Arends, Jan Willem; Putten, H.W.H.M. van der; Velde, G. P. M. Ten

doi:10.1002/(sici)1097-0339(199908)21:2<112::aid-dc6>3.0.co;2-d

Cited by 3 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This example confirms the limits of the analysis as a function of the material representativity. 14 In our study, all of the ACAs were aneuploid, but reports in the literature mentioned adenocarcinomatous effusions presenting a high rate of diploid cells. 15 The interpretation of histograms is variable among different studies, material collected, and different examination.…”

Section: Discussionmentioning

confidence: 46%

Image cytometry: An aid for cytological diagnosis of pleural effusions

2005

View full text Add to dashboard Cite

The conventional cytology rate for identification of neoplastic cells in effusions is about 60%. The rate of diagnostically equivocal effusions in routine cytology is dependent on the volume of effusion examined, type of preparation and staining, experience of the examiner, and application of ancillary methods. The aim of our study was to confirm the role of image cytometry analysis (DNA-ploidy) on pleural effusions. In this retrospective study based on 42 available cases with a histological diagnosis, we have examined 13 reactive mesothelial proliferations and 29 cases of malignant tumors (adenocarcinoma [ACA] or mesothelioma). The smears collected were submitted to the image analysis following a-three step protocol (smears stained with the Papanicolaou method were destained and then restained with Feulgen staining and finally analysed using image analysis cytometry). The results have shown that nonmalignant cases (reactive mesothelial proliferation) were all diploid and in contrast all aneuploid cases corresponded to malignant tumors. Only three mesotheliomas showed a diploid profile. In conclusion, these results confirm data from literature and indicate that cytometric analysis of nuclear content is a useful marker for identification of malignant cells in equivocal effusions and can be used to increase the cytological sensitivity in doubtful mesothelial proliferations.

show abstract

Section: Discussionmentioning

confidence: 46%

Image cytometry: An aid for cytological diagnosis of pleural effusions

2005

View full text Add to dashboard Cite

show abstract

“…It has also been suggested that the additional diagnostic value of DNA image cytometry in cases with a cytological diagnosis of ''atypia'' or ''suspicious for malignancy'' is limited (39), but that visual diagnostic cytology and morphometric digital microscopy miss some cases of malignancy, which can be detected by DNA FCM (4). Moreover, hyperdiploidy can be attributed to mesothelial elements, as was demonstrated by a combined analysis of FISH and immunocytochemistry with staining for cytokeratin (23).…”

Section: Discussionmentioning

confidence: 99%