Clinical Virological and Pathological Investigations on Horses With Neurologic Disorders in Turkey

Yılmaz, Hüseyin; Gürel, Aydın; Aktaş, Mustafa Sinan; Yıldırım, Funda; Bamaç, Özge Erdoğan; Haktanır, Damla; Tekelioğlu, Bilge Kaan; Gur, Emre; Tarakci, Eda Altan; Helps, Christopher R; Richt, Jüergen A.; Turan, Nuri

doi:10.1016/j.jevs.2017.08.006

Cited by 1 publication

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“…Symptoms like upper respiratory tract disorders, coughing, sneezing, nasal discharges, weakening, neuropathogenic symptoms, imbalance, incoordination, and nodding have been observed in animals affected by herpesviruses (Newton et al, 2000;Yilmaz et al, 2018). Histopathological examinations of fetuses following EHV-1-related abortion cases have revealed necrotic lesions in the liver and lung, intranuclear inclusion bodies in cells, germinal center necrosis, eosinophilic inclusion bodies, petechial hemorrhage in the brain, bronchiolitis, and pneumonia, clear yellow fluid accumulation in the brain, and pulmonary congestion and edema (Murphy et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Detecting Equine Herpesvirus-1 and Equine Herpesvirus -4 Infections in Racehorses using Polymerase Chain Reaction

TEKELİOĞLU,

AKAY

2023

Black Sea Journal of Health Science

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The aim of this study was to detect and differentiate equine herpes virus type-1 (EHV-1) and type-4 (EHV-4) by polymerase chain reaction (PCR). The diagnostic sensitivity of the PCR was also examined. For this purpose, suspicious samples obtained from different breeding farms and racehorse hospitals were used. During this study, multiple organ and tissue samples of 26 aborted fetuses were collected and analyzed with PCR. In addition, 98 nasal swab specimens from racehorses aged 2 and above representing the respiratory symptoms of EHV infections were collected and examined by the PCR method. This study started with the optimization of PCR using EHV-1 (89c25p) and EHV-4 (TH20p) DNA, which are reference strains. It was observed that the gB common oligonucleotide primers and species-specific gC primers used in optimization matched the viral DNA specific for target EHV-1 and EHV-4.Finally 7 EHV-1 positives at tissues of aborted fetuses of 26 and 1 EHV-1 and 2 EHV-4 positives at nasal swab samples of 98 has been detected and differentiated by PCR. This was the first PCR study to detect and differentiate EHV-1 and EHV-4 in Turkey. Results of this study reveal the presence of EHV-1 and EHV-4 in Turkey. Especially the presence of EHV-4 virus has been shown for the first time. As a result of the study, detection of EHV-1 and EHV-4 genomic DNAs by PCR indicated that both viruses circulate between racehorse population.In conclusion, we agreed that the PCR is a sensitive, economical and time-saving method in detecting and differentiating EHV-1 and EHV-4 infections. Key words:EHV-1, EHV-4, infection, Polymerase Chain Reaction (PCR)

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Section: Introductionmentioning

confidence: 99%