Clinicopathologic Correlations of Retrocorneal Membranes Associated With Endothelial Corneal Graft Failure

Naranjo, Andrea; Pirakitikulr, Nathan; Pelaez, Daniel; Sabater, Alfonso L.; Monsalve, Pedro; Amescua, Guillermo; Dubovy, Sander R.

doi:10.1016/j.ajo.2020.08.016

Cited by 5 publications

(3 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…12 Kremer et al 3 found a high correlation between glaucoma tube-shunt operations and the presence of diffuse membranes, with up to 37% of failed full-thickness grafts having at least focal fibrous ingrowth in that series. A more recent study postulated that fibrocellular retrocorneal membranes may be associated with DSAEK failure in patients with tube shunts 13 but was observational in design and limited to 7 patients. An important context for these findings is the increased risk of graft failure associated with glaucoma surgery itself.…”

Section: Discussionmentioning

confidence: 99%

Risk Factors for Fibrous Ingrowth in Eyes Requiring Primary Keratoplasty

Ramirez,

Walling,

Fortenbach

et al. 2023

Cornea

View full text Add to dashboard Cite

Purpose: The aim of this study was to define risks for corneal transplantation associated with fibrous ingrowth among first-time transplant recipients. Methods: We performed a retrospective case–control study of patients with a histopathologic diagnosis of fibrous ingrowth between 2002 and 2019. Patients with fibrous ingrowth from a first corneal specimen were included. Those with incomplete records were excluded. A 1:2 case–control ratio was used. Controls were matched using surgical indication, surgery year, transplantation method, sex, and age. Results: Seventy-eight eyes (76 patients) were included and matched with 160 control eyes. The incidence of fibrous ingrowth found on a first corneal transplant was 0.6% per year. The most common keratoplasty indications were pseudophakic corneal edema (n = 25, 32%) and aphakic corneal edema (n = 15, 19%). Cases were more likely to have a history of ocular trauma (odds ratio [OR], 2.94; 95% CI, 1.30–6.30; P = 0.007), uveitis (OR, 2.73; 95% CI, 1.12–6.63; P = 0.022), retinal detachment or previous retinal surgery (OR, 2.40; 95% CI, 1.34–4.30; P = 0.003), glaucoma tube-shunt surgery (OR, 2.70; 95% CI, 1.29–5.65; P = 0.007), aphakia (OR, 3.02; 95% CI, 1.61–5.67; P = 0.0004), or iris derangement (OR, 10.52; 95% CI, 5.45–20.30; P <0.0001). A multivariate logistic regression model using iris derangement, history of ocular trauma, history of uveitis, and history of cataract surgery demonstrated 81% sensitivity and 66% specificity in predicting presence of fibrous ingrowth. Conclusions: A history of ocular trauma, uveitis, retinal detachment or previous retinal surgery, glaucoma tube-shunt surgery, aphakia, and iris derangement are risks for detecting fibrous ingrowth among first-time keratoplasty recipients. Patients with these conditions should be monitored closely for corneal decompensation.

show abstract

Section: Discussionmentioning

confidence: 99%

Risk Factors for Fibrous Ingrowth in Eyes Requiring Primary Keratoplasty

Ramirez,

Walling,

Fortenbach

et al. 2023

Cornea

View full text Add to dashboard Cite

show abstract

“…The samples were incubated with the appropriate secondary antibody (goat anti-rabbit AlexaFluor ®® 488, ab150077, Abcam, Cambridge, MA, 1:100; goat anti-guinea pig Alexa Fluor ®® 488, ab150185, Abcam, Cambridge, MA, 1:100) for 2 h. The samples were washed three times with PBS and counterstained with 1X PureBlu DAPI (BioRad, Hercules, CA, USA) for 5 min, then mounted. The slides were then viewed using a fluorescence microscope [ 46 ].…”

Section: Methodsmentioning

confidence: 99%

Valdecoxib Protects against Cell Apoptosis Induced by Endoplasmic Reticulum Stress via the Inhibition of PERK-ATF4-CHOP Pathway in Experimental Glaucoma

Gao

Yao

et al. 2022

IJMS

View full text Add to dashboard Cite

The purpose of this study was to investigate the effects of valdecoxib on the retina in retinal ischemia-reperfusion injury (IRI) and R28 cells following oxygen-glucose deprivation/recovery (OGD/R) injury, as well as the underlying mechanisms. Immunofluorescence and Cell Counting Kit-8 (CCK-8) analyses were used to identify the proper timepoint and concentration of valdecoxib’s protective effect on the R28 cells in the OGD/R model. Hematoxylin-eosin (HE) staining and immunofluorescence were used to explore valdecoxib’s effect on the retina and retina ganglion cell (RGC) in IRI. Cell apoptosis was determined by a TUNEL Apoptosis Detection Kit and Annexin V-FITC/PI flow cytometry. The expression levels of p-PERK, transcription factor 4 (ATF4), GRP78, CHOP, cleaved caspase 3, bax and bcl-2 were measured by Western blot analyses. The valdecoxib protected the R28 cells from OGD/R injury by decreasing the cell apoptosis rate, and it exerted a protective effect on retinas in I/R injury by inhibiting RGC apoptosis. The valdecoxib pretreatment reversed the expression of p-PERK, ATF4, CHOP, GRP78, cleaved caspase 3 and bax induced by the glaucomatous model. Meanwhile, the CCT020312 reversed the valdecoxib’s anti-apoptosis effect by activating PERK-ATF4-CHOP pathway-mediated endoplasmic reticulum (ER) stress. These findings suggest that valdecoxib protects against glaucomatous injury by inhibiting ER stress-induced apoptosis via the inhibition of the PERK-ATF4-CHOP pathway.

show abstract

“…Als Ursprung für die fibröse RCM, die erstmals 1901 durch den österreichischen Augenarzt Professor Ernst Fuchs beschrieben wurde [2,6], wurden eine stromale Zellinvasion durch eine nicht vollständige Spender-Empfänger-Adhärenz [10] und/oder endothelial-mesenchymale Transformation vermutet [1,3,5,6]. In einer aktuellen Studie von Naranjo et al [7] waren die immunhistologischen Marker für die endothelial-mesenchymale Transformation positiv, was die wichtige Rolle dieses Mechanismus für die Pathogenese der fibrösen RCM unterstreicht. In beiden Fällen kommt es zu einer unkontrollierten Synthese von Bindegewebe mit nachfolgender Endotheldegeneration und -apoptose im Sinne eines nichtimmunologischen Transplantatversagens [1].…”

Section: Infoboxunclassified