Clinicopathological Significance of S100A14 Expression in Lung Adenocarcinoma

Abstract

Background: Several studies have reported that S100A14 plays important roles during different steps of the tumorigenic process and tumor progression of several types of cancer. The aim of the present study was to investigate the clinicopathological and prognostic significance and functional roles of S100A14 in lung adenocarcinoma. Patients and Methods: S100A14 expression was immunohistochemically studied in 166 consecutive resected lung adenocarcinomas, and its correlation with clinicopathological parameters was evaluated. Functional roles of S100A14 in lung adenocarcinoma were investigated based on invasion and migration assays on a small interfering (si)RNA-treated lung adenocarcinoma cell line. Results: S100A14 expression was detected in 82 of the 166 (49.4%) lung adenocarcinomas. S100A14 expression was significantly correlated with sex, poorer tumor differentiation, higher disease stages, larger tumor size, lymph node metastasis, intratumoral vascular invasion, intratumoral lymphatic invasion, pleural invasion, and poorer prognosis. Invasion and wound healing assays showed that S100A14 siRNA knockdown cells had significantly decreased invasion and migration abilities compared with siRNA control cells. Conclusion: S100A14 is expressed in a subset of lung adenocarcinoma, and its expression is related to certain clinicopathological parameters. Furthermore, S100A14 expression was strongly correlated with migration and invasion in lung adenocarcinoma cells.

Overexpression of S100A14 contributes to malignant progression and predicts poor prognosis of lung adenocarcinoma

“…and Hu et al . found that S100A14 overexpression is associated with tumor metastasis and S100A14 knockdown by RNA interference leads to dramatically reduced migration and invasion of these cells . However, Zhu et al .…”

“…10 Katono et al and Hu et al found that S100A14 overexpression is associated with tumor metastasis and S100A14 knockdown by RNA interference leads to dramatically reduced migration and invasion of these cells. 11,12 However, Zhu et al found that S100A14 expression is negatively correlated to cell migration and invasion in gastric cancer. S100A14 blocks store-operated Ca2+ influx by inhibiting Orai1 and STIM1 expression, leading to FAK activation, MMP downregulation, and focal adhesion assembly.…”

A Clinicopathological Analysis of S100A14 Expression in Colorectal Cancer

“…First of all, there are several studies trying to identify how S100A14 is implicated in tumorigenesis by conducting experiments on cancer cell lines. However, it seems that S100A14 affects tumorigenic processes in a different way in different types of malignancies (13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27). In particular, S100A14 enhances proliferation and cell growth in breast (13), cervical (17), ovarian (18) and hepatocellular cancer (19), whereas it inhibits them in oral (23), esophageal (24) and urothelial cancer (27).…”

“…Furthermore, S100A14 facilitates cell migration in breast (14-16), cervical (17), ovarian (18), hepatocellular (19) and lung cancer (20,21), while it restrains it in gastric cancer (26). Similarly, under the influence of S100A14, motility is increased in breast cancer (15,16), but decreased in urothelial cancer (27), and invasiveness is increased in breast 16, cervical (17), ovarian (18), hepatocellular (19) and lung cancer (20,21), but decreased in oral (22) and gastric cancer (26). Finally, S100A14 seems to promote cell differentiation in esophageal (24) and gastric cancer (26), while it mediates epithelial-to-mesenchymal transition in cervical cancer (17).…”

“…Concerning the comparison between normal and malignant tissues, S100A14 expression is higher in malignant tissues in breast (13,19), ovarian (30), hepatocellular (19) and lung cancer (20), whereas it is lower in malignant tissues in colorectal cancer (34). As far as disease stage is concerned, high S100A14 levels are associated with advanced stages in cervical (17), ovarian (18), hepatocellular (19) and lung cancer (20,21), while they are associated with early stages in small bowel cancer (33). In addition, S100A14 expression is increased as the depth of infiltration is increased in lung cancer (20), whereas the exact opposite is true for gastric cancer (26).…”