CLOCK:BMAL-Independent Circadian Oscillation of Zebrafish Cryptochrome1a Gene

Miyamura, Norio; Hirayama, Jun; Sawanobori, K; Tamaru, Teruya; Asaoka, Yoshiyuki; Honda, Reiko; Yamamoto, Takuro; Uno, Hatsume; Takamatsu, Ken; Nishina, Hiroshi

doi:10.1248/bpb.32.1183

Cited by 6 publications

(3 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cell Culture, Enzyme Inhibition and Light Induction Cultured zebrafish cells were prepared as described previously 8,17) and maintained at 28°C in L-15 medium (SigmaAldrich) containing 10% fetal bovine serum. For inhibitor experiments, cells were cultured in the above medium for 2 d in constant darkness prior to treatment (in the dark) for 60 min with dimethyl sulfoxide (DMSO) (vehicle control), 1 mM or 10 mM diphenyleneiodonium chloride (DPI), or 10 mM or 100 mM L-NAME.…”

Section: Methodsmentioning

confidence: 99%

Diphenyleneiodonium Chloride, an Inhibitor of Reduced Nicotinamide Adenine Dinucleotide Phosphate Oxidase, Suppresses Light-Dependent Induction of Clock and DNA Repair Genes in Zebrafish

Osaki

Uchida

Hirayama

et al. 2011

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

In most species, solar light is both a DNA-damaging agent and the key entraining stimulus for the endogenous circadian clock. The zebrafish is an attractive vertebrate system in which to study the influence of light on gene expression because the DNA repair proteins and circadian oscillators in this species are light-responsive. At the molecular level, light treatment of zebrafish cells induces the production of reactive oxygen species (ROS). ROS both alters the reduction-oxidation (redox) state of these cells and stimulates intracellular extracellular signal-regulated kinase (ERK)/mitogen activated protein kinase (MAPK) cascades that transduce photic signals activating the transcription of particular light-responsive genes, including some clock genes and some DNA repair genes involved in photoreactivation. To date, however, the phototransducing molecules responsible for light-dependent ROS production have not been identified. Flavin-containing oxidases, such as reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase, are versatile flavoenzymes that catalyze molecular oxidation in numerous metabolic pathways. Importantly, light induces the photoreduction of the flavin adenine dinucleotide (FAD) moiety in these oxidases, leading to ROS production. Here, we show in cultured zebrafish cells that diphenyleneiodonium chloride (DPI), an inhibitor of NADPH oxidase, both suppresses ERK/MAPK activation and efficiently reduces light-dependent expression of clock and photoreactivation genes. Our results suggest that flavin-containing oxidases may be responsible for light-dependent ROS production and thus light-dependent gene expression in zebrafish. Our findings also support the existence of a regulatory link between photoreactivation and the circadian clock in this species.

show abstract

Section: Methodsmentioning

confidence: 99%

Diphenyleneiodonium Chloride, an Inhibitor of Reduced Nicotinamide Adenine Dinucleotide Phosphate Oxidase, Suppresses Light-Dependent Induction of Clock and DNA Repair Genes in Zebrafish

Osaki

Uchida

Hirayama

et al. 2011

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, the persistence of rapidly damping rhythmic expression for cry1a and per2 following transfer from LD to constant conditions points to an element of clock regulation for both genes [27,60]. However, in the case of cry1a there is some uncertainty as to whether this involves a Clock:Bmal‐dependent mechanism [27,66]. Another study has also pointed to the involvement of AP1 enhancer elements in directing light‐induced cry1a expression [67].…”

Section: Light Entrainable Peripheral Clocksmentioning

confidence: 99%

It's time to swim! Zebrafish and the circadian clock

et al. 2011

View full text Add to dashboard Cite

The zebrafish represents a fascinating model for studying key aspects of the vertebrate circadian timing system. Easy access to early embryonic development has made this species ideal for investigating how the clock is first established during embryogenesis. In particular, the molecular basis for the functional development of the zebrafish pineal gland has received much attention. In addition to this dedicated clock and photoreceptor organ, and unlike the situation in mammals, the clocks in zebrafish peripheral tissues and even cell lines are entrainable by direct exposure to light thus providing unique insight into the function and evolution of the light input pathway. Finally, the small size, low maintenance costs and high fecundity of this fish together with the availability of genetic tools make this an attractive model for forward genetic analysis of the circadian clock. Here, we review the work that has established the zebrafish as a valuable clock model organism and highlight the key questions that will shape the future direction of research.

show abstract

“…57,63,64) Indeed, there is evidence that light-induced zCRY1a is a transcriptional repressor essential for the light-dependent entrainment of the circadian clock. 63) The light-dependent transcription of zCry1a is controlled through the production and removal of cellular reactive oxygen species (ROS).…”

Section: A Model Of a Potential Molecular Mechanism Underlying Lighmentioning

confidence: 99%

A Common Origin: Signaling Similarities in the Regulation of the Circadian Clock and DNA Damage Responses

Uchida

Hirayama

Nishina

2010

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

INTRODUCTIONFrom bacteria to humans, almost all organisms can adapt the timing of their physiology to the cyclic changes of their environment, thanks to a naturally-selected intrinsic timekeeping system called the circadian clock. 1) The circadian clock enhances the physiological efficiency and survival of an organism by organizing its behavior and body functions. 2,3) During the circadian day, the organism's physiology is given over to catabolic processes, whereas the anabolic functions of growth, repair and consolidation occur at night. To achieve this schedule in mammals, the circadian clock regulates a number of physiological functions, including sleep and wakefulness, food intake, body temperature, cardiovascular and renal activity, hormone production, hepatic metabolism and immune responses. 4,5) Accordingly, disruption of the circadian clock in humans has been linked to profound effects on health, including insomnia, stomach ailments, depression and cancer. 4,5) In most organisms, the molecular mechanisms underlying the establishment and maintenance of biological rhythms comprise interconnected transcription-translation feedback loops in which some clock factors repress their own transcription once they have attained critical levels. 2,3) These oscillators have the property of being endogenous and cellautonomous systems that maintain their rhythm in the absence of external time cues. 6) Both vertebrates and invertebrates have circadian oscillators scattered throughout their bodies. 7,8) In mammals, the circadian system is composed of both central and peripheral oscillators. 7) The mammalian central clock is located in the suprachiasmatic nucleus (SCN) within the anterior hypothalamus of the brain. 9) This central clock acts as a coordinator and provides time signals via both neural and humoral routes that entrain independent peripheral clocks. Dysfunction of the central clock does not inactivate the peripheral clocks but instead causes individual peripheral oscillators to become temporally uncoupled. [10][11][12] To guarantee that an organism's behavior remains tied to the rhythms of its environment, the circadian clock must be able to reset itself in response to environmental cues. 9,13,14) The main environmental stimulus for organisms is light, which is provided in day-night cycles. Mammals have no photoreceptors in peripheral tissues, 15) so that the effect of light on peripheral clocks is indirect. 13) For the mammalian clock, the SCN integrates photic cues from the retina and uses neural and humoral signals to transmit this information to peripheral clocks, synchronizing them. [16][17][18] This communication between the central and peripheral clocks results in the seamless regulation of fundamental physiological functions. 4,8) Interestingly, peripheral clocks can also respond directly to SCN-independent signals such as feeding and temperature change. 19,20) However, the physiological role of SCN-independent responses of peripheral clocks is not yet fully understood. A recent study has reported that ...

show abstract

CLOCK:BMAL-Independent Circadian Oscillation of Zebrafish Cryptochrome1a Gene

Cited by 6 publications

References 26 publications

Diphenyleneiodonium Chloride, an Inhibitor of Reduced Nicotinamide Adenine Dinucleotide Phosphate Oxidase, Suppresses Light-Dependent Induction of Clock and DNA Repair Genes in Zebrafish

Diphenyleneiodonium Chloride, an Inhibitor of Reduced Nicotinamide Adenine Dinucleotide Phosphate Oxidase, Suppresses Light-Dependent Induction of Clock and DNA Repair Genes in Zebrafish

It's time to swim! Zebrafish and the circadian clock

A Common Origin: Signaling Similarities in the Regulation of the Circadian Clock and DNA Damage Responses

Contact Info

Product

Resources

About