Clodronate stimulates osteoblast differentiation in ST2 and MC3T3-E1 cells and rat organ cultures

Itoh, Fumiaki; Aoyagi, Shigemi; Furihata-Komatsu, Hanako; Aoki, M.; Kusama, Hiroshi; Kojima, Masami; Kuroda, Hiroshi

doi:10.1016/j.ejphar.2003.08.011

Cited by 39 publications

(29 citation statements)

References 38 publications

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“…Particularly, for alendronate (Im et al, 2004), osteoblast proliferation and maturation are promoted at concentrations of 10 -8 M, but inhibition of cellular growth is obtained at concentrations in the order of 10 -4 M (Long et al, 2009). However, other studies disagreed and reported that alendronate had no effects on osteoblast differentiation using a similar interval concentration (Itoh et al, 2003). The findings of the present study support the statement of Long et al (2009).…”

Section: In Vivo Biocompatibilitysupporting

confidence: 80%

Biocompatibility of alendronate-loaded acrylic cement for vertebroplasty

Calvo-Fernández

Parra²,

Fernández-Gutiérrez³

et al. 2010

eCM

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This paper reports a biological evaluation of a nonresorbable acrylic cement loaded with alendronate for the treatment of osteoporotic vertebral compression fractures. The cement formulation was based on polymethyl methacrylate and acrylic monomers; one of these had covalently linked vitamin E residues. The same cement in the absence of alendronate was used as a control. The setting of the charged cement presented a maximum polymerization temperature of 44ºC, a setting time of 24 min, a residual monomer content lower than 3 wt.%, a compressive strength of 99±10 MPa and an elastic modulus of 1.2±0.2 GPa. Cytotoxicity studies using human osteoblast cultures revealed that the leachable substances of the alendronate loaded cement collected between 1 and 7 days decreased cell viability to values lower than 80%. However, morphological changes and cellular damage in cells produced by the extracts decreased with the leak time. Cell adhesion and growth on charged cement was significantly lower than on the control. Implantation of the cement paste in the intra-femoral cavity of rabbits showed that initially the osteogenic activity was evident for the cement charged with alendronate, and the osteosynthesis process took place mainly in the trabeculae and was manifested by the presence of a non-mineralised osseous spicule. The interface between material and adjacent bone tissue was initially characterized by a variable fibrous response that in many cases it appeared reduced to thin connective tissue after a 24-week-period.

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Section: In Vivo Biocompatibilitysupporting

confidence: 80%

Biocompatibility of alendronate-loaded acrylic cement for vertebroplasty

Calvo-Fernández

Parra²,

Fernández-Gutiérrez³

et al. 2010

eCM

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“…The explanation for these changes is not clear, especially because the authors reported that osteocalcin levels, a marker of bone formation, were not elevated. Bisphosphonates have been shown to have direct effects on osteoblast function, and it is possible that the high doses used in these studies, as well as the protocol of repeated dosing in growing animals, may have contributed to the enhanced bone accretion at these sites (38)(39)(40). An increase in trabecular bone volume with ZA was also apparent in the CIA model, and these results warrant further investigation.…”

mentioning

confidence: 88%

Bisphosphonates: Environmental protection for the joint?

Goldring¹,

Gravallese²

2004

Arthritis & Rheumatism

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“…In particular, clodronate promotes osteoblast differentiation in cultures of osteoblast-like line cells, such as ST2 and MC3T3-E1 cells, and in rat organ cultures, while etidronate stimulates osteoblast differentiation only in MC3T3-E1 cells [66]. Moreover, etidronate stimulates osteoblastogenesis and wound closure in rat calvaria [67].…”

Section: Bps and Osteoblastsmentioning

confidence: 98%