Clomiphene citrate ameliorated lead acetate-induced reproductive toxicity in male
            Wistar rats

Oyeyemi, Wahab Adekunle; Princely, Anyanwu C; Akinola, Adeniran Oluwadamilare; Ore-Oluwapo, Daramola O; Blessing, Adeola O.; Ehiaghe, Friday A.

doi:10.5935/1518-0557.20190038

Cited by 24 publications

(39 citation statements)

References 35 publications

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“…Administration of ALA led to increase this ratio levels in the T2 group, also it could be increased this ratio in the groups T3 that was exposed to lead compared with the control levels, may be attributed to a potential antioxidant properties of ALA in overwhelm the adverse effect of lead (23) via inhibiting the generation of hydroxy radicals that attack sulphur-containing antioxidants to sustain the levels of protein thiols and modulate tissue endogenous antioxidants (24). Lead has been found to cause a significant decrease in testosterone hormone concentration through the experimental periods in T1 group, this result was consistent with that recorded by Wahab et al, (25). A decreased in testosterone could be resulted by the effect of lead acetate on the axial of hypophysis-pituitary-testes and/or by decreasing in the activity of testicular steroidogenic enzymes, such as 3betahydroxysteroid dehydrogenase (3β-HSD), 17betahydroxy dehydrogenase (17β-HSD) resulted to Sertoli and Leydig cells insufficiency causes testicular damage and decrease in testosterone synthesis (6,8).…”

Section: Discussionsupporting

confidence: 85%

Role of alpha lipoic acid in protecting testes of adult rats from lead toxicity

Al-Okaily¹,

Murad²

2021

IJVS

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The current study was conducted to investigate the role of alpha lipoic acid (ALA) against testicular toxicity-induced by lead acetate (PbAc) in rats. Four groups of adult Wistar albino rats (8 for each) were intubated daily for 56 days as follows; control (C)received dislled water; lead acetate at dose of 5mg/kg b.w (T1); ALA at dose of 60mg/kg b.w (T2) and and group T3 received both of PbAc + ALA at the same doses of above. Blood samples were collected at 0, 28 and 56 day of the experiment, then the sera were collected for determination of testosterone (T) and follicular stimulating hormone (FSH). At the end of the experiment, body weight, testes weight and epididymal sperm parameters was studied. Furthermore, histo-morphometric and histopathological study changes were examined. The results revealed a significant decrease in testes weight to body weight ratio, serum testosterone, sperm concentration and motility, diameters of seminiferous tubules, height of seminiferous epithelia and number of Leydig cells, moreover the results showed a significant increase in serum FSH, dead sperm and abnormal sperm morphology in group T1 when compared with the other groups. Comparing to lead acetate treated rats, group T3 showed an improvement at the level of the studied parameters, accompanied with mild congestion in the interstitial tissue with a marked developing proliferation of spermatogenic cells, as well as presence of mature spermatozoa in the seminiferous tubules. In conclusion, subchronic exposure of rats to lead acetate showed an amelioration of all reproductive parameters near to normal values due to the antioxidant effects of ALA and the histological changes of the testes confirmed such change in serum parameters and the beneficial role of ALA.

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Section: Discussionsupporting

confidence: 85%

Role of alpha lipoic acid in protecting testes of adult rats from lead toxicity

Al-Okaily¹,

Murad²

2021

IJVS

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“…Another study described the effects of lead on the hypothalamic–pituitary–testicular axis, steroidogenesis, spermatozoa parameters, and testicular antioxidant enzyme activity of male Wistar rats after an administration of lead acetate [ 112 ]. In this study, adult male Wistar rats received lead (10 mg/kg) had decreased serum luteinizing hormone (LH) and testosterone levels; testicular 17β-hydroxysteroid dehydrogenase (HSD) activity; androgen receptor expression; spermatozoa motility, viability and counts; catalase activity (CAT); and SOD when compared with controls.…”

Section: Lead (Pb)mentioning

confidence: 99%

“…In this study, adult male Wistar rats received lead (10 mg/kg) had decreased serum luteinizing hormone (LH) and testosterone levels; testicular 17β-hydroxysteroid dehydrogenase (HSD) activity; androgen receptor expression; spermatozoa motility, viability and counts; catalase activity (CAT); and SOD when compared with controls. Abnormal spermatozoa morphology and MDA increased significantly in the lead experimental group [ 112 ].…”

Section: Lead (Pb)mentioning

confidence: 99%

Effects of Cadmium, Lead, and Mercury on the Structure and Function of Reproductive Organs

Massányi

Madeddu

et al. 2020

Toxics

146

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Reproductive organs are essential not only for the life of an individual but also for the survival and development of the species. The response of reproductive organs to toxic substances differs from that of other target organs, and they may serve as an ideal “barometer” for the deleterious effects of environmental pollution on animal and human health. The incidence of infertility, cancers, and associated maladies has increased in the last fifty years or more, while various anthropogenic activities have released into the environment numerous toxic substances, including cadmium, lead, and mercury. Data from epidemiological studies suggested that environmental exposure to cadmium, lead, and mercury may have produced reproductive and developmental toxicity. The present review focused on experimental studies using rats, mice, avian, and rabbits to demonstrate unambiguously effects of cadmium, lead, or mercury on the structure and function of reproductive organs. In addition, relevant human studies are discussed. The experimental studies reviewed have indicated that the testis and ovary are particularly sensitive to cadmium, lead, and mercury because these organs are distinguished by an intense cellular activity, where vital processes of spermatogenesis, oogenesis, and folliculogenesis occur. In ovaries, manifestation of toxicity induced by cadmium, lead, or mercury included decreased follicular growth, occurrence of follicular atresia, degeneration of the corpus luteum, and alterations in cycle. In testes, toxic effects following exposure to cadmium, lead, or mercury included alterations of seminiferous tubules, testicular stroma, and decrease of spermatozoa count, motility and viability, and aberrant spermatozoa morphology.

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“…Lead can decrease the weights of the body, testes, epididymes, prostate and seminal vesicle (Pinon‐Lataillade et al., 1995). Numerous studies documented that lead exposure has been linked to altered sperm parameters which subsequently leads to male infertility (Kumar, 2018; Martin et al., 2017; Wahab et al., 2019). Searching for protective therapeutic agents against lead‐induced reproductive toxicity has been a great interest in scientific research.…”

Section: Introductionmentioning

confidence: 99%

Ameliorative effect of L‐carnitine on chronic lead‐induced reproductive toxicity in male rats

Abdel‐Emam

Ahmed

2021

Veterinary Medicine & Sci

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Lead toxicity is one of the causative agents of male infertility that raised concern from environmental contamination worldwide. L-carnitine, a biologically active amino acid, present in high concentration in the reproductive organs such as the epididymis, is involved in sperm maturation. The possible protective effect of L-carnitine in experimentally lead-induced male reproductive toxicity in rats was evaluated in this study.Thirty adult male Wistar rats were divided into three groups. Group 1: the negative control group was treated with normal saline; Group 2: exposed to 50 mg/kg lead acetate (2% solution in saline); and Group 3: treated with lead acetate 50 mg/kg (2% solution in saline) + L-carnitine 100 mg/kg. At the end of the experimental period, body and testicular weights were determined, blood samples were withdrawn for hormonal assays of FSH, LH and testosterone. Sperm parameters as sperm count, morphology, viability and motility were measured. Testicular tissue homogenates were prepared for enzymatic assays and for measuring oxidative stress parameters.Lead significantly increased both oxidative stress and the concentration of lactate dehydrogenase-C in the testicular tissues with a decrease in sperm count, motility and viability. Lead acetate treatment, induced alteration in sperms with normal morphology together with reductions in the serum FSH, LH, testosterone, body and testicular weights. The concentration of 17β-hydroxysteroid dehydrogenase was significantly reduced. Co-administration of L-carnitine significantly reduced testicular oxidative stress, improved sperm parameters, elevated serum FSH, LH and testosterone with an insignificant reduction in the testicular weight. The concentrations of 17β-hydroxysteroid dehydrogenase and lactate dehydrogenase-C were significantly improved by L-carnitine. The overall results indicate that L-carnitine is expected to improve the lead acetate-induced male reproductive toxicity.

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Clomiphene citrate ameliorated lead acetate-induced reproductive toxicity in male Wistar rats

Cited by 24 publications

References 35 publications

Role of alpha lipoic acid in protecting testes of adult rats from lead toxicity

Role of alpha lipoic acid in protecting testes of adult rats from lead toxicity

Effects of Cadmium, Lead, and Mercury on the Structure and Function of Reproductive Organs

Ameliorative effect of L‐carnitine on chronic lead‐induced reproductive toxicity in male rats

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