Clonal Dissemination of Linezolid-Resistant Staphylococcus haemolyticus Exhibiting the G2576T Mutation in the 23S rRNA Gene in a Tertiary Care Hospital in Brazil

Almeida, Lara Mendes de; Lincopán, Nilton; Araújo, Maria; Mamizuka, Elsa Masae

doi:10.1128/aac.06089-11

Cited by 14 publications

(9 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Only three isolates obtained from patients (4, 7 & 13) showed a similar pattern indicating emergence from the same clone. These findings suggest that different clones of LRCoNS may be circulating in the hospital which is in contrast to other published studies where all isolates showed similar PFGE banding pattern [17, 23, 24].…”

Section: Discussioncontrasting

confidence: 96%

Linezolid resistant coagulase negative staphylococci (LRCoNS) with novel mutations causing blood stream infections (BSI) in India

et al. 2019

View full text Add to dashboard Cite

Background Coagulase-negative Staphylococci (CoNS) have emerged as a major causative agent of blood-stream infections (BSI). Linezolid (LZD) is currently used for treating glycopeptide and methicillin-resistant staphylococci. It is important to understand the resistance mechanism and probable transmission of LZD resistant (LR) CoNS within the hospital. Methods Clinically significant LRCoNS from patients with BSI were characterized using MALDI-TOF and 16S rRNA gene sequence analysis. Antimicrobial susceptibility and MIC of vancomycin and LZD were determined. LZD resistance mechanisms using PCR for the cfr gene and mutation in the V domain of the 23S rRNA gene were studied. Results The MIC of LZD ranged from 8 to 32 μg/ml. LR was observed in three different CoNS species from diverse locations within the hospital. The cfr gene was identified in all the isolates. Sequence analysis of V domain region of 23S rRNA gene confirmed mutation in single copy among 12/15 isolates with novel mutations: G2614 T and C2384T. All infections were nosocomially acquired and LZD resistance was emerging in the absence of prior LZD use. Horizontal spread of resistant isolates and cfr gen e among diverse species were the probable mechanisms of transmission. Conclusion The study highlights the novel mutations associated with LRCoNS and the importance of surveillance & transmission pathway within the hospital. It also systematically discusses the published information on LRCoNS.

show abstract

Section: Discussioncontrasting

confidence: 96%

Linezolid resistant coagulase negative staphylococci (LRCoNS) with novel mutations causing blood stream infections (BSI) in India

et al. 2019

View full text Add to dashboard Cite

show abstract

“…The most common mechanism of resistance to linezolid among LRCoNS from Brazil is the G2576T mutation in 23S rRNA gene. The cfr gene has not been detected to date (7,8,30,31).…”

Section: Discussionmentioning

confidence: 86%

Emergence of methicillin‐resistant coagulase‐negative staphylococci resistant to linezolid with rRNA gene C2190T and G2603T mutations

Cidral¹,

Carvalho²,

Figueiredo³

et al. 2015

APMIS

View full text Add to dashboard Cite

The aim of this article were to determinate the mechanism of linezolid resistance in coagulase-negative methicillin-resistant staphylococci from hospitals in the northeast of Brazil. We identified the isolates using VITEK(®) 2 and MALDI-TOF. Susceptibility to antibiotics was measured by the disk-diffusion method and by Etest(®) . Extraction of the whole genome DNA was performed, followed by screening of all the strains for the presence of mecA and cfr genes. The domain V region of 23S rRNA gene was sequenced and then aligned with a linezolid-susceptible reference strain. Pulsed-field gel electrophoresis (PFGE) macro-restriction analysis was performed. Three linezolid-resistant Staphylococcus hominis and two linezolid-resistant Staphylococcus epidermidis strains were analyzed. The isolates showed two point mutations in the V region of the 23S rRNA gene (C2190T and G2603T). We did not detect the cfr gene in any isolate by PCR. The S. hominis showed the same pulsotype, while the S. epidermidis did not present any genetic relation to each other. In conclusion, this study revealed three S. hominis and two S. epidermidis strains with resistance to linezolid due to a double mutation (C2190T and G2603T) in the domain V of the 23S rRNA gene. For the first time, the mutation of C2190T in S. epidermidis is described. This study also revealed the clonal spread of a S. hominis pulsotype between three public hospitals in the city of Natal, Brazil. These findings highlight the importance of continued vigilance of linezolid resistance in staphylococci.

show abstract

“…The genotype distributions of staphylococci differ across different geographical regions. The USA300 community-acquired methicillin-resistant S. aureus clone sequence typing ST8 is dominant in the United States, as the ST80 and ST30 clones are in Europe, Kuwait, Singapore, the Southwest Pacific islands and New Zealand (Udo et al 2008), and Brazilian epidemic clone ST239 that is predominant in different hospitals (Carvalho et al 2010, Almeida et al 2012. Regarding S. epidermidis, the ST69 clone is predominant in Greece,ST57 and ST88 in Portugal,ST2 in China,ST2,ST22,ST61 and ST71 in Mexico and ST63 in Argentina (Miragaia et al 2009, Liakopoulos et al 2010).…”

mentioning

confidence: 99%

“…The detection of methicillinresistant strains belonging to this virulent clonal lineage in hospitalised -mainly immunocompromised -patients points to possible hospital infections, as these patients had been hospitalised for more than 72 h, they did not come from other hospitals, they received no prolonged antibiotic therapy at the moment of collection and they presented with frequent use of catheters because of their treatments. Therefore, studies monitoring the resistance of these strains to antibiotics are essential in light of the reports of coagulase-negative staphylococci species resistant to linezolid therapy, a therapeutic option for multidrug-resistant Gram-positive pathogens, including methicillin-resistant S. aureus and vancomycin-resistant enterococci (Almeida et al 2012).…”

mentioning

confidence: 99%

Novel methicillin-resistant coagulase-negative Staphylococcus clone isolated from patients with haematological diseases at the Blood Bank Centre of Amazon, Brazil

Ferreira

Naveca

Ferreira³

et al. 2013

Mem. Inst. Oswaldo Cruz

View full text Add to dashboard Cite

Clonal Dissemination of Linezolid-Resistant Staphylococcus haemolyticus Exhibiting the G2576T Mutation in the 23S rRNA Gene in a Tertiary Care Hospital in Brazil

Cited by 14 publications

References 12 publications

Linezolid resistant coagulase negative staphylococci (LRCoNS) with novel mutations causing blood stream infections (BSI) in India

Linezolid resistant coagulase negative staphylococci (LRCoNS) with novel mutations causing blood stream infections (BSI) in India

Emergence of methicillin‐resistant coagulase‐negative staphylococci resistant to linezolid with rRNA gene C2190T and G2603T mutations

Novel methicillin-resistant coagulase-negative Staphylococcus clone isolated from patients with haematological diseases at the Blood Bank Centre of Amazon, Brazil

Contact Info

Product

Resources

About