Clonal diversity of Clostridium perfringens human clinical isolates with various toxin gene profiles based on multilocus sequence typing and alpha-toxin (PLC) typing

Aung, Meiji Soe; Matsuda, Asami; Urushibara, Noriko; Kawaguchiya, Mitsuyo; Ohashi, Nobuhide; Matsuda, Norifumi; Nakamura, Mayumi; Ito, Masahiko; Habadera, Satoshi; Matsumoto, Akinori; Hirose, Mina; Kobayashi, Nobumichi

doi:10.1016/j.anaerobe.2021.102473

Cited by 4 publications

(10 citation statements)

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“…In terms of strains of human origin, the isolates recovered from human diarrhea cases (n = 111) were found to be of type A (n = 102), type F (n = 8), and type D (n = 1). Type A and type F were the major toxinotypes in the isolates, basically in accordance with earlier reports (Aung et al, 2021;Wang et al, 2021). Interestingly, there was one isolate of type D that carried cpe and/or cpb2 genes, indicating that it had the potential to produce the CPE toxin (Kiu and Hall, 2018).…”

Section: Discussionsupporting

confidence: 90%

Molecular characteristics and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

et al. 2023

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Clostridium perfringens (C. perfringens) is a significant foodborne pathogen and a common cause of intestinal diseases in both animals and humans. Our study investigated MLST, phenotypic antimicrobial resistance profiles, and resistance genes among isolates from human, animal and food. 186 C. perfringens isolates were obtained from nine provinces in China between 2013 and 2021. Additionally, some specific ST complexes were analyzed by cgMLST and cgSNP to investigate genetic relatedness. MLST indicated the most prevalent STs of C. perfringens of human and animal origin were as follows: ST221 (5/147), ST62 (4/147), ST408 (4/147), and ST493 (4/147) were predominant in humans, while ST479 (5/25) was the major type in animals. Within the same ST complex, genetically unrelated relationships or potential clustering/transmission events were further recognized by cgMLST and cgSNP, illustrating that these two methods are valuable in defining outbreaks and transmission events. All tested isolates were susceptible to vancomycin and meropenem. The rates of resistance to metronidazole, penicillin, cefoxitin, moxifloxacin, and chloramphenicol were low (metronidazole: 1.08%; penicillin: 9.68%; cefoxitin: 0.54%; moxifloxacin: 6.45%; and chloramphenicol: 3.76%). Interestingly, 49.66% of human origin were clindamycin-resistant, and 18.2% were penicillin-insensitive. Importantly, the portion of MDR isolates was significantly lower than in previous reports. The study provides an overview of the epidemiological characteristics of C. perfringens with different origins and hosts in China. C. perfringens demonstrated remarkable genetic diversity and distinct molecular features compared to antibiotic-resistance profiles from other studies.

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Section: Discussionsupporting

confidence: 90%

Molecular characteristics and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

et al. 2023

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“…In terms of strains of human origin, the isolates recovered from human diarrhea cases (n = 111) were found to be of type A (n = 102), type F (n = 8), and type D (n = 1). Type A and type F were the major toxin types in the isolates, basically in accordance with earlier reports (20, 19). Interestingly, there was one isolate of type D that carried cpe and/or cpb2 genes, indicating that it had the potential to produce the CPE toxin (1).…”

Section: Discussionsupporting

confidence: 92%

“…The MLST analysis of Chinese strains indicated a wide genetic diversity of C. perfringens , as previously reported (17). In our study, the predominant STs from human isolates (ST221, ST62, ST408) were significantly distinct from the predominant STs (ST479) from animal isolates, a result that was consistent with previous research on global C. perfringens strains, indicating a diverse distribution of C. perfringens among multiple sources (18, 19). In global surveys, a clonal complex (CC) represented by three STs (ST 98, ST 41, and ST 110) predominantly representing type F (18/20 strains) was mostly associated with human illnesses (18).…”

Section: Discussionsupporting

confidence: 92%

“…The copyright holder for this this version posted September 24, 2022. ; https://doi.org/10.1101/2022.09.23.509295 doi: bioRxiv preprint ST77 were associated with human illnesses (18,19). Consistent with these findings, we observed that the strains ST41 (2/2, type A) and ST77 (2/2, type F) were isolated from patients with diarrhea.…”

Section: Discussionsupporting

confidence: 86%

“…ST221 was the dominant genotype of C. perfringens among the isolates, with significant genetic diversity. Previous results revealed that ST41 and ST77 were associated with human illnesses (18, 19). Consistent with these findings, we observed that the strains ST41 (2/2, type A) and ST77 (2/2, type F) were isolated from patients with diarrhea.…”

Section: Discussionmentioning

confidence: 96%

See 2 more Smart Citations

Molecular characters and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

Zhong

Zheng

Wang

et al. 2022

Preprint

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Clostridium perfringens (C. perfringens) is a significant foodborne pathogen and a common cause of intestinal diseases in both animals and humans. Altogether, 186 isolates were obtained from humans (n = 147), animals (n = 25), and food (n = 14), comprising 174 type A strains (93.55%), 11 type F strains (5.91%), and one type D strain (0.54%); and were analyzed by multilocus sequence typing (MLST) and antimicrobial susceptibility testing. Additionally, some specific ST complexes were analyzed by cgMLST and cgSNP to investigate genetic relatedness. MLST indicated the most prevalent STs of C. perfringens of human and animal origin were as follows: ST221 (5/147), ST62 (4/147), ST408 (4/147), and ST493 (4/147) were predominant in humans, while ST479 (5/25) was the major type in animals. Within the same ST complex, genetically unrelated relationships or potential clustering/transmission events were further recognized by cgMLST and cgSNP, illustrating that these two methods are valuable in defining outbreaks and transmission events. All tested isolates were susceptible to vancomycin and meropenem. The rates of resistance to metronidazole, penicillin, cefoxitin, moxifloxacin, and clindamycin were low (metronidazole: 1.08%; penicillin: 9.68%; cefoxitin: 0.54%; moxifloxacin: 6.45%; and chloramphenicol: 3.76%). Interestingly, 49.66% of human origin were clindamycin-resistant, and 18.2% were penicillin-insensitive. Importantly, the portion of MDR isolates was significantly lower than in previous reports. The study provides an overview of the epidemiological characteristics of C. perfringens with different origins and hosts in China. C. perfringens demonstrated remarkable genetic diversity and distinct molecular features compared to antibiotic-resistance profiles from other studies.

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Nontyping virulence factors of Clostridium perfringens

Svobodová,

Hulánková

2024

Acta Vet. Brno

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The anaerobic, rod-shaped, spore-forming bacterium Clostridium perfringens is known for its production of biochemically active substances. Most of them are proteins that have a pathogenic effect on a wide range of animal tissues and cause a specific syndrome or even a disease in humans or animals. Production of toxins is used to classify isolates of C. perfringens into 7 different toxin types (A–G). Other virulence factors (i.g. beta2-toxin, BEC toxin, sialidases, hyaluronidase etc.) only indirectly or partially participate in the development of the disease, and the function of some substances has not been fully elucidated. The article summarizes basic data on the non-typing virulence factors of C. perfringens.

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Clonal diversity of Clostridium perfringens human clinical isolates with various toxin gene profiles based on multilocus sequence typing and alpha-toxin (PLC) typing

Cited by 4 publications

References 38 publications

Molecular characteristics and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

Molecular characteristics and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

Molecular characters and phylogenetic analysis of Clostridium perfringens from different regions in China, from 2013 to 2021

Nontyping virulence factors of Clostridium perfringens

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