Clonal diversity of methicillin-resistant Staphylococcus aureus (MRSA) from bloodstream infections in northern Japan: Identification of spermidine N-acetyltransferase gene (speG) in staphylococcal cassette chromosomes (SCCs) associated with type II and IV SCCmec

Aung, Meiji Soe; Urushibara, Noriko; Kawaguchiya, Mitsuyo; Hirose, Mina; Ito, Masahiko; Habadera, Satoshi; Kobayashi, Nobumichi

doi:10.1016/j.jgar.2020.12.008

Cited by 27 publications

(15 citation statements)

References 30 publications

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“…The most common clone in northern Japan, in the Hokkaido region, was very different from other regions, and the percentage of SCC mec type II was 71.4%. This is similar to the surveillance results conducted in the Hokkaido region between 2017 and 2019 [16], although the number of medical institutions that participated in the Hokkaido region in this study was only two. Thus, there may be regional differences in the spread of ST8-IV and ST1-IV.…”

Section: Discussionsupporting

confidence: 89%

Changing molecular epidemiology and characteristics of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections: nationwide surveillance in Japan in 2019

Kaku

Sasaki

Ota

et al. 2021

Preprint

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ObjectivesSome single-center studies have reported that methicillin-resistant Staphylococcus aureus (MRSA) carrying the staphylococcal cassette chromosome mec (SCCmec) type IV has been increasing in bloodstream infections (BSIs) in Japan. Therefore, we conducted nationwide surveillance for MRSA BSI to verify that there is a change all over Japan.MethodsWe recruited 51 Japanese hospitals from the Japanese Association for Infectious Diseases. MRSA strains, which were detected in two or more sets of blood, were collected between January and September 2019. They were analyzed by antimicrobial susceptibility testing at Nagasaki University Hospital. Whole-genome sequencing was also performed to determine SCCmec typing and multilocus typing and detect drug-resistance and virulence genes.Results270 MRSA strains were collected from 45 hospitals. The major clones were ST8 with SCCmec type IV (ST8-IV) (30.7%), ST1-IV (29.6%), ST2725-IV (9.5%), ST764-II (8.1%), and ST5-II (7.8%). However, there were regional differences among the most major clones. The most common clones in western, eastern, and northern Japan were ST1-IV, ST8-IV, and ST5-II, respectively. ST8-IV, ST1-IV, and ST2725-IV exhibited lower drug resistance against clindamycin and minocycline than ST764-II and ST5-II, but erm(A) was detected in 93.8% and 100% of ST1-IV and ST2725-IV, respectively. Based on drug-resistance and virulence genes, characteristics of ST8-IV were different from those of ST1-IV and ST2725. In addition, the two major types were expected to be ST8-IV.ConclusionsThis study revealed that SCCmec type IV clones replaced SCCmec type II in MRSA BSI. In addition, three main types of SCCmec type IV clones have spread throughout Japan.

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Section: Discussionsupporting

confidence: 89%

Changing molecular epidemiology and characteristics of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections: nationwide surveillance in Japan in 2019

Kaku

Sasaki

Ota

et al. 2021

Preprint

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“…In Japan, since the first detection of this clone in 2007 [ 51 ], a low prevalence of this clone (0.2–3.1%) has been reported in community and healthcare settings [ 42 , 43 , 52 , 53 ]. Nevertheless, an increasing trend was also noted [ 53 ], and 5.1% of the isolates from blood samples were identified as this clone in our recent study [ 44 ]. Therefore, associated with the persistent spread of the USA300 clone among the Japanese population, ST6562 is suggested to have emerged as a local variant.…”

Section: Discussionmentioning

confidence: 74%

“…Among the clinical isolates of MRSA from healthcare and community settings in Hokkaido, Japan, coa -IIa (CC5) is predominant, and coa -IIIa (CC8) and coa -VIIa (CC1, CC59) are also commonly detected [ 42 , 43 , 44 ]. Unlike these dominant clones, three MRSA isolates in the present study (ST8-SCC mec IVl, ST4775-SCC mec IVa, ST6562-SCC mec IVa) had unique characteristics.…”

Section: Discussionmentioning

confidence: 99%

“…The ST8-SCC mec IVl isolate, which was derived from a young dental patient (13y) in the present study, was PVL-negative and harbored sec , sep , tst-1 and spj , which was characteristic of the CA-MRSA/J clone that emerged in Japan in 2003 as a cause of skin infections among children [ 31 ]. Despite the low prevalence, this clone has been identified among the CA- and HA-MRSA isolated from all ages in the Japanese population, occasionally leading to severe diseases [ 42 , 43 , 44 , 46 , 47 ]. Moreover, this clone was demonstrated in the oral cavity of healthy children in our previous study [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

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Antimicrobial Resistance and Molecular Epidemiological Characteristics of Methicillin-Resistant and Susceptible Staphylococcal Isolates from Oral Cavity of Dental Patients and Staff in Northern Japan

et al. 2021

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The acquisition of drug resistance and virulence by staphylococcal species colonizing humans is a growing public health concern. The present study was conducted to investigate the prevalence, antimicrobial resistance and genetic characteristics of Staphylococcus isolates from the oral cavity and skin (hand) of systemically healthy subjects with dental disease and dental staff in northern Japan. Among a total of 133 subjects (91 patients and 42 staff), 87 coagulase-positive Staphylococcus (83 S. aureus/4 S. argenteus) and 162 coagulase-negative Staphylococcus (CoNS) isolates were recovered from 59 (44.4%) and 95 (71.4%) subjects, respectively. Three oral isolates were methicillin-resistant S. aureus (MRSA) (3.6%, 3/83) that were genotyped as ST8-SCCmec-IVl, ST4775(CC1)-SCCmec-IVa and ST6562(CC8)-SCCmec-IVa. Remarkably, the ST6562 isolate harbored PVL genes on ΦSa2usa and type I ACME (arginine catabolic mobile element). Four methicillin-susceptible isolates were identified as S. argenteus belonging to ST1223 and ST2250, which harbored enterotoxin genes egc-2 and sey, respectively. Among the fourteen CoNS species identified, methicillin-resistant (MR) isolates were detected in five species (11 isolates, 13.3% of CoNS), with S. saprophyticus and S. haemolyticus being the most common. ACME was prevalent in only S. epidermidis and S. capitis. These findings indicated the potential distribution of USA300 clone-like MRSA, toxigenic S. argenteus and MR-CoNS in the oral cavity of dental patients.

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“…ST1-t1784 MRSA isolates from human blood samples have been previously reported in Japan ( Aung et al., 2021 ). In the phylogenetic tree based on SNPs, the ST1-t1784 isolates obtained in this study shared the origin with a strain isolated from a human in Japan (accession no.…”

Section: Discussionmentioning

confidence: 99%

Outbreak of methicillin-resistant Staphylococcus aureus sequence type 1, spa type t1784, in an equine hospital in Japan

Uchida‐Fujii

Niwa²,

Kanai³

et al. 2022

Veterinary and Animal Science

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Clonal diversity of methicillin-resistant Staphylococcus aureus (MRSA) from bloodstream infections in northern Japan: Identification of spermidine N-acetyltransferase gene (speG) in staphylococcal cassette chromosomes (SCCs) associated with type II and IV SCCmec

Cited by 27 publications

References 30 publications

Changing molecular epidemiology and characteristics of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections: nationwide surveillance in Japan in 2019

Changing molecular epidemiology and characteristics of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections: nationwide surveillance in Japan in 2019

Antimicrobial Resistance and Molecular Epidemiological Characteristics of Methicillin-Resistant and Susceptible Staphylococcal Isolates from Oral Cavity of Dental Patients and Staff in Northern Japan

Outbreak of methicillin-resistant Staphylococcus aureus sequence type 1, spa type t1784, in an equine hospital in Japan

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