Clonal effects in propagating oak trees via in vitro culture

Abstract

Shoot growth, shoot multiplication in vitro, and rooting of shoots were compared in 6 clones derived from adult trees originating from a single plot in a french forest and in 16 clones derived from seedlings originating in the NE of France or in the Netherlands. The results showed strong within-provenance variations affecting shoot growth, multiplication factor, number of subcultures that could be achieved and, for the clones from adult trees only, frequency of shoots that rooted. In contrast, significant between-provenance differences could not be shown.

Why and How Using Micropropagated Trees rather than Germinations for Controlled Synthesis of Ectomycorrhizal Associations?

“…As for primary cultures, genetic origin plays a determining role in the establishment of a long-term culture (Chalupa 1988;Juncker and Favre 1989;Meier-Dinkel et al 1993). In many cases, new developed shoots degenerated after a successful primary culture or even after several subcultures for no apparent reason.…”

Vegetative propagation of oak (Quercus robur and Q petraea) by cutting and tissue culture

“…Micropropagated plantlets were transplanted into soil and later were planted in the field. The system of axillary-shoot multiplication was used for micropropagation of various oak species: Q robur and Q petraea (Chalupa, 1979(Chalupa, , 1981(Chalupa, , 1983(Chalupa, , 1984(Chalupa, , 1985(Chalupa, , 1987b(Chalupa, , 1988(Chalupa, , 1990bVietez et al 1985;Pevalek-Kozlina and Jelaska 1986;Civinová and Sladky, 1987;Favre and Juncker, 1987;Meier-Dinkel, 1987;SanJosé et al 1988SanJosé et al , 1990Juncker and Favre, 1989;Volkaert et al, 1990), Q suber (Bellarosa, 1981(Bellarosa, , 1989Pardos, 1981; Manzanera and Pardos, 1990), Q Shumardii (Bennett and Davies, 1986), Q acutissima (Ide and Yamamoto, 1986;Sato et al, 1987), Q serrata (Ide and Yamamoto, 1987) and Q lobata (Johnson and Walker, 1990 (Chalupa, 1985(Chalupa, , 1987a(Chalupa, , 1990cJörgensen, 1988), Q suber (El Maataoui and Espagnac, 1987), Q acutissima (Sasaki et al, 1988), Q rubra and Q alba (Gingas and Lineberger, 1989) (Gresshoff and Doy, 1972), BTM (Chalupa, 1984), or Woody plant medium (WPM) (Lloyd and McCown, 1980). The basal media were supplemented with glutamine (100 mg·l -1 ).…”

Micropropagation and ex vitro rooting of several clones of late-flushing Quercus robur L

“…Shoots derived form subcultured shoot tips and nodal segments had a low rooting and survival rate (21 %) after 4 months. 56% of shoots derived from subcultured basal segments with a callus, rooted (Chalupa, 1984(Chalupa, , 1988 (Vieitez et al, 1985;Evers et al, 1987;San José et al, 1988, 1990Juncker and Favre, 1989) as well as plants in soil (Meier-Dinkel, 1987;Chalupa, 1988) (Jörgensen, 1990 …”