Clonal micropropagation of a rare species Hedysarum theinum Krasnob. (Fabaceae) and assessment of the genetic stability of regenerated plants using ISSR markers

In vitro propagation of endemic species Hedysarum chaiyrakanicum (Tuva Republic, Russia) and its widespread congener, H. gmelini (Fabaceae)

“…Earlier for H. theinum Krasnob. [10,11] we have found the formation of adventitious buds at the shoot base for the BAP medium only on the 4th and 5th subcultivations; the reproduction rate was 9.2±1.1 pcs./exp., which could be treated as an evidence of accumulation the growth regulator in the explant tissues. While in vitro cultivating of H. grandiflorum Pall.…”

“…The mineral base of MS medium has previously been used to cultivate valuable medicinal species H. theinum [10,11,13], and successfully introduced into the practice of cultivating other Hedysarum species [12,14,15].…”

“…We studied the BAP effect on the morphogenetic potential of H. chaiyrakanicum and H. gmelini during the 4 subcultivations, which has been described as an essential component for H. theinum Krasnob. [10,11], H. grandiflorum Pall. and H. argyrophyllum Ledeb.…”

Conservación de plantas regeneradas in vitro y análisis de la variación somaclonal de Cinchona officinalis, Linneo

“…Similar respuesta se obtuvo en Hydrangea macrophylla cuando la misma combinación de reguladores no presentó riesgo de variación somaclonal en los explantos (Liu et al, 2011). En los explantes de Hedysarum theinum también con regeneración adventicia no se afectó al genotipo (Erst et al, 2015) y en Nothapodytes foetida, tampoco se encontró variación en los regenerantes obtenidos de manera adventicia (Chandrika et al, 2010). En varios trabajos se ha reportado que son varios los factores que influyen en la variación genética entre ellos la vía regenerativa de manera indirecta, sin embargo no es regla el hecho que los cultivos de regeneración directa estables tienen menor riesgo de variación somaclonal y los que pasan por una fase de callo presentan mayor probabilidad de variación (Tang, 2001;Gao et al, 2010;Bairu et al, 2011).…”

Features of propagation by seeds of rare and endangered plant species in culture

“…Unfortunately, biotechnological methods are not sufficiently applied. Using these methods, a large number of regenerants can be obtained from a small number of source material [2,3]. One of the results of creating a collection of RE species is the formation of stable introduction populations with a large number of individuals.…”