Clonal population structure and introductions of the chestnut blight fungus, Cryphonectria parasitica, in Asturias, northern Spain

González-Varela, Germán; González, Ana J.; Milgroom, Michael G.

doi:10.1007/s10658-011-9788-0

Cited by 22 publications

(24 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition to the vic genes, the host genetic background has been shown to affect virus transmission (Cortesi et al 2001). In clonal populations, the transmission of the virus can take place more readily between individuals due to the lack of genetic diversity González-Varela et al 2011). This may explain the better conversion rate with isolates from León, which included a small number of different vc types and only one idiomorph (Mat-1) (Zamora et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Cryphonectria parasitica isolates of the same vegetative compatibility type display different rates of transfer of CHV1 hypovirus

et al. 2015

View full text Add to dashboard Cite

Hypovirulent strains of the chestnut blight fungus Cryphonectria parasitica have not been registered in the autonomous region of Castilla y León (Spain), except in the province of León. In this laboratory-based study, we analyzed the rates of horizontal transmission of hypovirus CHV1 subtype F1, isolated from chestnut stands in León. We tested the conversion capacity of the six vegetative compatibility (vc) types of C. parasitica isolates most commonly distributed in Castilla y León (EU1, EU11, EU12, EU66, CL5 and CL6). We investigated conversion rates of virulent isolates into hypovirulent isolates between pairings of isolates of the same vc type (EU1, EU11 and CL6) and also tested cross conversion rates between isolates of different vc types (EU1, EU11 and CL6 as donors and EU12, EU66, CL5 and CL6 as recipients). We carried out the hypovirus transmission assay with 1700 pairings, of which 700 had donor and recipient isolates of the same vc type and 1000 pairings of isolates had different vc types. Our results show that the conversion frequency to hypovirulent isolates was significantly affected by the vc type, the genotype of isolates with the same origin (province) and the interaction between both factors. In the conversion between isolates of the same vc type, the conversion rates were better with EU1 (ranging between 56 and 94 %) than with EU11 (varying from 4 to 58 %). In the cross conversion between donor and recipient isolates of different vc types, only CL5 and CL6 recipients were converted and the conversion of recipient isolates of EU12 and EU66 failed in all cases. For CL5 as recipient isolate, the conversion rates were similar with EU11 and CL6 as the donor isolates. Recipient isolates of CL6 had good conversion with donor isolates from the same vc type (CL6) and in cross conversion the results were better with donor EU1. Fungal isolates from chestnut stands in León displayed the best conversion rates, followed by those from Zamora, Salamanca and Ávila when the donor and the recipient isolates were of the same vc type. The fungal isolates appear to have a strong influence on the transmission rate of the hypovirus, at least between strains isolated from chestnut stands in Castilla y León. EU1 isolates were more susceptible to conversion than the isolates from EU11. The results highlight the differences in hypovirus transmission, regarding vc types and the genotype of isolates from the same province, when Eur J Plant Pathol donor and recipient isolates are of the same vc type. Further transmission assays would be useful to determine why hypovirus transmissions, when compared to the hypovirus transmission of other European assays, have different conversion rates with fungal isolates from Castilla y León.

show abstract

Section: Discussionmentioning

confidence: 99%

Cryphonectria parasitica isolates of the same vegetative compatibility type display different rates of transfer of CHV1 hypovirus

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Asturias, the region north of León, has also EU1 in common with Castilla y León but differs by having EU3 and EU13 (González‐Varela et al. ).…”

Section: Discussionmentioning

confidence: 99%

Diversity of Cryphonectria parasitica in western Spain and identification of hypovirus‐infected isolates

Zamora

Martín²,

Rigling

et al. 2012

Forest Pathology

View full text Add to dashboard Cite

Summary Isolates of the chestnut blight fungus Cryphonectria parasitica were obtained from 44 localities in four provinces in Western Spain and characterized for vegetative compatibility (vc) types, mating types and the presence of Cryphonectria hypovirus 1 (CHV1). Among the 1232 isolates recovered from chestnut blight cankers, 11 vc types were identified: five known vc types included in EU1 to EU74 (EU1, EU11, EU12, EU28 and EU66) and six unknown vc types (CL4, CL5 CL6, CL8, CL9 and CL10). The number of vc types found per province varied between two and seven. The vc type EU11 was present in all provinces and accounted for 48.9% of all isolates. EU1 was detected in three provinces and accounted for 39.1% of the isolates. The vc types EU12, EU66, CL5 and CL6 were present in one or two provinces and comprised between 2.4% and 3% of the isolates. The other vc types were represented by only one or very few isolates. The mating type MAT‐1 was largely dominant in the provinces Leon and Avila, while both mating types MAT‐1 and MAT‐2 were found in Salamanca and Zamora. Fourteen hypovirus‐infected C. parasitica isolates were found, nine were in vc type EU1 and five in EU11, and they were detected only in the province León. All isolates analysed contained the French hypovirus subtype CHV1‐F1.

show abstract

“…Furthermore, increasing global trade in plant products carries the danger of introduction of pathogens, providing recurrent opportunities for new invasions [5]–[7]. These pathogens have the potential to cause severe economic losses to crops, ornamental plants, or forests, and can lead to severe problems in human or livestock nutrition [8]–[9].…”

Section: Introductionmentioning

confidence: 99%

“…Despite the importance of these invasions, relatively little is known about the modes, timing or frequency with which they have occurred. Knowledge of these factors is important for prediction, prevention, and response to additional introductions [9]; [14]. Molecular genetic data can be useful for the evaluation of the reasons for invasion success.…”

Section: Introductionmentioning

confidence: 99%

Global Genetics and Invasion History of the Potato Powdery Scab Pathogen, Spongospora subterranea f.sp. subterranea

et al. 2013

View full text Add to dashboard Cite

Spongospora subterranea f. sp. subterranea (Sss) causes two diseases on potato (Solanum tuberosum), lesions on tubers and galls on roots, which are economically important worldwide. Knowledge of global genetic diversity and population structure of pathogens is essential for disease management including resistance breeding. A combination of microsatellite and DNA sequence data was used to investigate the structure and invasion history of Sss. South American populations (four countries, 132 samples) were consistently more diverse than those from all other regions (15 countries, 566 samples), in agreement with the hypothesis that Sss originated in South America where potato was domesticated. A substantial genetic differenciation was found between root and tuber-derived samples from South America. Estimates of past and recent gene flow suggested that Sss was probably introduced from South America into Europe. Subsequently, Europe is likely to have been the recent source of migrants of the pathogen, acting as a “bridgehead” for further global dissemination. Quarantine measures must continue to be focussed on maintaining low global genetic diversity and avoiding exchange of genetic material between the native and introduced regions. Nevertheless, the current low global genetic diversity of Sss allows potato breeders to select for resistance, which is likely to be durable.

show abstract

Clonal population structure and introductions of the chestnut blight fungus, Cryphonectria parasitica, in Asturias, northern Spain

Cited by 22 publications

References 31 publications

Cryphonectria parasitica isolates of the same vegetative compatibility type display different rates of transfer of CHV1 hypovirus

Cryphonectria parasitica isolates of the same vegetative compatibility type display different rates of transfer of CHV1 hypovirus

Diversity of Cryphonectria parasitica in western Spain and identification of hypovirus‐infected isolates

Global Genetics and Invasion History of the Potato Powdery Scab Pathogen, Spongospora subterranea f.sp. subterranea

Contact Info

Product

Resources

About