Clonal propagation of Enset (Ensete superbum (Roxb.) Cheesman) through shoot tip cultures

Mathew, M. M.; Philip, V. J.

doi:10.1007/bf01890874

Cited by 6 publications

(1 citation statement)

References 5 publications

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“…But the report of Ponni and Ashalatha (2019) supports present investigation, where addition of IBA with BA reduced the shoot multiplication rate in Ensete superbum (Roxb.) Cheesman, whereas the other available report supports the usage of auxins with BA for multiple shoot induction in Ensete superbum (Mathew and Philip, 1996).…”

Section: Resultsmentioning

confidence: 92%

Micropropagation of Rauvolfia Tetraphylla L. From in Vitro Seedling Derived Explants Using Various Amino Acids

Anitha,

Sujatha,

Ranjitha Kumari

2023

THE JAPS

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Rauvolfia tetraphylla L. belonging to the family Apocynaceae has been used in the treatment of hypertension, snake bites and insect sting poisons since a long time. Though various attempts were reported for the regeneration of R. tetraphylla through explants collected from in vivo grown plants, this is the first attempt reporting regeneration from in vitro seedling derived explants. Various amino acids were also tested for their efficacy on in vitro regeneration of R. tetraphylla. The regeneration through the shoot tip and cotyledonary node explants of in vitro grown seedlings were achieved in MS+B5 medium fortified with 3% sucrose and 0.8% agar. By adjusting the cytokinin (BA (Benzyl adenine), KN (Kinetin)) concentrations and various amino acids (glutamine, lysine, proline, methionine and isoleucine) in multiple shoot induction medium, the media composition was optimized for obtaining maximum results. Compared to KN, BA gave best response for multiple shoot induction. Maximum number of shoots were produced in the cotyledonary nodes than the shoot tips. Amino acids encouraged multiple shoot induction (especially glutamine and proline) response when combined with 4.44 µM BA, where 34.75 and 29.18 shoots were produced per explant with 0.51 mM glutamine and 0.66 mM proline respectively. Elongation of shoots was 100 % on medium with 0.44 µM BA and 1.156 µM GA3 (Gibberellic acid). The rooting of elongated shoots (65%) was attained by incorporating IBA (Indole butyric acid) in the medium. The roots were thick, long, about 4.8 roots were produced per explant on medium supplied with 9.84 µM IBA. The hardening and field introduction attempts were successful with the survival rate of 78 % after 12 weeks of acclimatization.

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Section: Resultsmentioning

confidence: 92%

Micropropagation of Rauvolfia Tetraphylla L. From in Vitro Seedling Derived Explants Using Various Amino Acids

Anitha,

Sujatha,

Ranjitha Kumari

2023

THE JAPS

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Efficient micropropagation of Ensete ventricosum applying meristem wounding: a three-step protocol

Birmeta

Welander

2004

Plant Cell Rep

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A highly efficient three-step protocol for in vitro propagation of Ensete ventricosum (enset) was developed that consisted of initiation, bud proliferation, and shoot elongation and rooting stages. At the initiation stage, it was crucial to use shoot tips (5-8 mm) with subtending corm tissues as explants to obtain growth. The addition of 0.5-1% (w/v) activated charcoal to the medium was essential to prevent phenol exudation which otherwise leads to the loss of cultures. During the bud proliferation stage, modified MS macronutrients and micronutrients together with a combination of cytokinins (1.6 microM naphthaleneacetic acid, 4.4 microM 6-benzylaminopurine, 23.2 microM kinetin, 22.6 microM N(6) 2-isopentyladenine) was used. This novel composition of macronutrients was based on the analysis of leaf nutrient content of glasshouse-grown enset sprouts. Multiple bud formation on the enlarged corm tissue was induced only when the meristem region was wounded before transfer to the bud proliferation medium. Up to 75 healthy shoots per explant were produced, whereas unwounded explants produced, only one to two shoots per explant. A third stage with a low concentration of cytokinin enabled shoot elongation as well as root development. The plantlets were acclimatized with 100% success and they showed no apparent phenotypical deviation.

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Conservation protocols for Ensete glaucum, a crop wild relative of banana, using plant tissue culture and cryopreservation techniques on seeds and zygotic embryos

Singh

Thangjam

Harish

et al. 2020

Plant Cell Tiss Organ Cult

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Clonal propagation of Enset (Ensete superbum (Roxb.) Cheesman) through shoot tip cultures

Cited by 6 publications

References 5 publications

Micropropagation of Rauvolfia Tetraphylla L. From in Vitro Seedling Derived Explants Using Various Amino Acids

Micropropagation of Rauvolfia Tetraphylla L. From in Vitro Seedling Derived Explants Using Various Amino Acids

Efficient micropropagation of Ensete ventricosum applying meristem wounding: a three-step protocol

Conservation protocols for Ensete glaucum, a crop wild relative of banana, using plant tissue culture and cryopreservation techniques on seeds and zygotic embryos

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