Efficient micropropagation of Ensete ventricosum applying meristem wounding: a three-step protocol

Birmeta, Genet; Welander, Margareta

doi:10.1007/s00299-004-0832-9

Cited by 34 publications

(25 citation statements)

References 15 publications

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“…3b). Similar effects were observed in Ensete ventricosum (Birmeta and Welander, 2004) and Senecio macrophyllus (Trejgell et al, 2010b). Reducing the MS salts concentration has been shown to promote in vitro rooting of shoots in several other species (Joshi and Dhar, 2003;Baskaran et al, 2006).…”

supporting

confidence: 60%

An Efficient System for Regenerating Taraxacum Pieninicum Pawł. from Seedling Explants

Trejgell¹,

Chernetskyy²,

Podlasiak³

et al. 2013

Acta Biologica Cracoviensia Series Botanica

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An efficient micropropagation system for Taraxacum pieninicum using seedling explants germinated in vitro is described. Shoot tips and fragments of cotyledons, hypocotyls and roots were isolated from several-day-old seedlings. The highest response, 100% frequency with 12.3 axillary shoots/explant, was from shoot tips on medium supplemented with 0.5 mg L -1 BA and 0.05 mg L -1 NAA. In subsequent subcultures the number of shoots was significantly higher on all explants cultured on medium containing 0.25 and 0.5 mg L -1 BA, and the multiplication rate was highest (20 shoots/explant) in the 4th passage. Shoots rooted on MS and 1/2 MS medium; the highest rooting frequency was 90% and the highest number of roots 2.7/shoot. Rooted plants showed 96.2% survival in sterile soil:sand, and 100% survival in hydroponic culture. Regenerated plants flowered in the second year after acclimatization and yielded viable seeds. This protocol for obtaining complete plants through micropropagation may prove useful for conservation of the genetic resources of this and other endangered species.K Ke ey y w wo or rd ds s: : Taraxacum pieninicum, endangered species, in vitro culture, multiple shoots, benzylaminopurine, hydroponic culture, fertile plants, conservation.

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supporting

confidence: 60%

An Efficient System for Regenerating Taraxacum Pieninicum Pawł. from Seedling Explants

Trejgell¹,

Chernetskyy²,

Podlasiak³

et al. 2013

Acta Biologica Cracoviensia Series Botanica

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“…Birmeta and Welander (2004) reported that AC (5-10 g/l w/v) controlled phenol exudation from Ensete ventricosum (enset) that otherwise resulted in the loss of cultures. Tisserat (1979) found that 3 g/l AC increased the survival of palm explants (Phoenix dactylifera L.) and promoted organogenesis by reducing browning in the growth medium.…”

Section: Resultsmentioning

confidence: 99%

In vitro propagation of North American ginseng (Panax quinquefolius L.)

Uchendu

Paliyath

Brown

et al. 2011

In Vitro Cell.Dev.Biol.-Plant

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North American ginseng (NAG) (Panax quinquefolius L.) is a medicinally important plant with multiple uses in the natural health product industry. As seed propagation is time-consuming because of the slow growth cycle of the plant, in vitro propagation using a bioreactor system was evaluated as an effective approach to accelerate plant production. An efficient method was developed to multiply nodal explants of NAG using liquid-culture medium and a simple temporary immersion culture vessel. The effects of plant growth regulators, phenolics, and chemical additives (activated charcoal, melatonin, polyvinylpolypyrrolidone, and ascorbic acid) were evaluated on in vitro-grown NAG plants. The highest number (12) of shoots per single node was induced in half-strength Schenk and Hildebrandt basal medium containing 2.5 mg/l kinetin, in which 81% of the cultured nodes responded. In a culture medium with 0.5 mg/l α-naphthalene acetic acid (NAA), roots were induced in 78% of the explants compared to 50% with a medium containing indole-3-acetic acid. All of the resulting plants appeared phenotypically normal, and 93% of the rooted plants were established in the greenhouse. Phenolic production increased significantly (P< 0.05) over a 4-wk culture period with a negative impact on growth and proliferation. Activated charcoal (AC; 50 mg/l) significantly reduced total phenolic content and was the most effective treatment for increasing shoot proliferation. Shoot production increased as the phenolic content of the cultures decreased. The most effective treatment for NAG development from cultured nodal explants in the bioreactor was 2.5 mg/l kinetin, 0.5 mg/ l NAA, and 50 mg/l AC in liquid culture medium. This protocol may be useful in providing NAG tissues or plants for a range of ginseng-based natural health products.

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“…In Saussurea obvallata it increased the number of rooted shoots as well as the number of roots per shoot (Joshi and Dhar, 2003). Halving the MS salts boosted the rooting frequency in Eclipta alba (Baskaran and Jayabalan, 2005) and gave the best root growth from shoots in Ensete ventricosum (Birmeta and Welander, 2004). Root induction on shoots formed on BA-supplemented medium occurred a week later than for shoots differentiating on medium with ZEA and KN, and on full-strength MS medium supplemented with BA the number of roots per shoot was significantly lower (1.8 ± 0.2) than the corresponding shoots in the ZEA and KN treatments (Tab.…”

Section: Rooting and Survivalmentioning

confidence: 99%