Clonal Propagation of<i>Isoplexis canariensis</i>*

Schaller, Frank; Kreis, Wolfgang

doi:10.1055/s-2006-957937

Cited by 6 publications

(8 citation statements)

References 1 publication

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“…Almost every explant of most of the Digitalis species studied, including cells, protoplasts, and anthers, have the potential to regenerate plants through organogenesis or embryogenesis, e.g., [5,49]. Methods for the micropropagation of the species of the section Isoplexis have also been described [50][51][52]. Especially the effects of phytohormones and growth regulators had their fair share of attention.…”

Section: Biology and Physiologymentioning

confidence: 99%

The Foxgloves (Digitalis) Revisited

Kreis

2017

Planta Med

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This review provides a renewed look at the genus Digitalis. Emphasis will be put on those issues that attracted the most attention or even went through paradigmatic changes since the turn of the millennium. PubMed and Google Scholar were used (“Digitalis” and “Foxglove” were the key words) to identify research from 2000 till 2017 containing data relevant enough to be presented here. Intriguing new results emerged from studies related to the phylogeny and taxonomy of the genus as well as to the biosynthesis and potential medicinal uses of the key active compounds, the cardiac glycosides. Several Eastern and Western Foxgloves were studied with respect to their propagation in vitro. In this context, molecular biology tools were applied and phytochemical analyses were conducted. Structure elucidation and analytical methods, which have experienced less exciting progress, will not be considered here in great detail.

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Section: Biology and Physiologymentioning

confidence: 99%

The Foxgloves (Digitalis) Revisited

Kreis

2017

Planta Med

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“…The micropropagation pattern of Isoplexis was similar to that previously observed in woody species of Digitalis (Gavidia et al, 1996) and opens important possibilities for mass propagation of these species which, besides their cultural value, present a particular interest within studies focused on the biochemical pathway leading to the cardenolides. To date, morphogenesis from Isoplexis cultures has only been partially studied in I. canariensis (Schaller and Kreis, 1996;Arrebola et al, 1997).…”

Section: Micropropagationmentioning

confidence: 99%

LC‐NMR applied to the characterisation of cardiac glycosides from three micropropagated Isoplexis species

Gavidia¹,

Seitz²,

Pérez‐Bermúdez³

et al. 2002

Phytochemical Analysis

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Species of the genus Isoplexis are of particular interest with respect to the biochemical pathway leading to the cardenolides. It is important to determine whether or not 5beta-configured compounds, typically produced by Digitalis species and used in medicine, are present together with their respective alpha-isomers in Isoplexis spp. Structure elucidation by LC-NMR of the products isolated from in vitro regenerated Isoplexis canariensis, I. chalcantha and I. isabelliana was carried out, and similarities were observed among the products of the three species, including the presence of digitoxigenin-type cardenolides in I. canariensis and xysmalogenin and canarigenin derivatives in I. chalcantha never previously reported in these species. Pregnane glycosides not found until now either in Isoplexis or Digitalis were also detected.

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“…Plants were obtained from seeds or from shoot cultures [11] and maintained in the institute's greenhouse without artificial extra light and a temperature regimen of 25 8C/16 8C (day/night).…”

Section: Methodsmentioning

confidence: 99%

“…Shoot cultures were initiated from surface-sterilised seeds as described earlier [11]. The cultures were maintained on MS [15] medium containing 0.1 mg L -1 indole-3-acetic acid and 1 mg L -1 benzylaminopurine at 21 8C.…”

Section: Methodsmentioning

confidence: 99%

“…Plant tissue culture can be used for the propagation of endangered species such as I. chalcantha, native to a few places on Gran Canaria only. Protocols for the clonal propagation and regeneration of Isoplexis plants have been established [11], [13]. Plant tissue cultures may also provide useful tools for studying biosynthetic pathways (e. g., [14]) as long as the tissue culture under investigation for this purpose is capable of producing the compound(s) of interest.…”

Section: Introductionmentioning

confidence: 99%

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Cardenolide Genin Pattern in Isoplexis Plants and Shoot Cultures

Schaller¹,

Kreis²

2006

Planta med

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An HPLC method for the quantitative analysis of cardenolides was developed and applied. The procedure was optimised for analysing small samples (40 mg dw) of plant and tissue culture material. ISOPLEXIS CANARIENSIS plants obtained from seeds accumulated cardenolides to about 20 - 40 micromol g (-1) dw as calculated from the levels of cardenolide genins released after acidic hydrolysis of methanolic extracts. The relative contents of xysmalogenin, digitoxigenin, uzarigenin and canarigenin were 5 - 15 %, 0 - 5 %, 10 - 15 % and 70 - 90 %, respectively. Shoot cultures were initiated from seeds, established as permanent cultures and cultivated on agar-solidified or in liquid medium. Shoot cultures maintained on solid medium contained an average of about 6 micromol cardenolides g (-1) dw. A relatively high proportion of digitoxigenin was found in two-thirds of the shoot cultures examined. The cardenolide content of amphibian shoot cultures averaged to about 1 micromol g (-1) dw. Plants regenerated from shoot cultures and maintained under hydroponic conditions accumulated the same amount of cardenolides as plants collected in the field. No cardenolides could be detected in callus cultures.

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Clonal Propagation ofIsoplexis canariensis*

Cited by 6 publications

References 1 publication

The Foxgloves (Digitalis) Revisited

The Foxgloves (Digitalis) Revisited

LC‐NMR applied to the characterisation of cardiac glycosides from three micropropagated Isoplexis species

Cardenolide Genin Pattern in Isoplexis Plants and Shoot Cultures

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