Clonal Relationship of Salmonella enterica Serovar Typhimurium Phage Type DT104 in Germany and Austria

Prager, Rita; Liesegang, Annette; Rabsch, Wolfgang; Gericke, B.; Thiel, W; Voigt, W.; Helmuth, Reiner; Ward, L. R.; Tschäpe, Helmut

doi:10.1016/s0934-8840(99)80081-4

Cited by 45 publications

(42 citation statements)

References 19 publications

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“…This is supported by the fact that their DNA amplification fingerprints and their PFGE data (Baggesen et al, 2000;Izumiya et al, 2001) also show a uniform picture. The clonal identity of about 95% of DT104 isolates was found to occur in sensitive as well as a range of MDR variants (Prager et al, 1999). In contrast, Markogiannikis et al (2000) supposed the existence of 6 clones among Greek DT104 strains, primarily on the basis of PFGE; the dominant type 'A' was carrying the two integrons typical of DT104 (0.9 and 1.1 kb).…”

Section: Discussionmentioning

confidence: 98%

Integron content of Salmonella enterica serotype Typhimurium strains isolated in Hungary in the years 1997-1999

Gadó

Pászti

Király

et al. 2003

Acta Veterinaria Hungarica

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The integron content of 52 DT104/U302 phage type strains and 53 non-DT104/U302 strains of Salmonella enterica serotype Typhimurium (S. Typhimurium) was studied in PCR experiments using a 5'-CS/3'-CS primer pair (Lévesque et al., 1995). Forty-three out of 44 streptomycin-and/or ampicillinresistant DT104 and related phage type strains were found to carry a 1 kb and/or 1.2 kb long integron. The other resistance markers did not affect the number and size of integrons; no integron-free multidrug-resistant (MDR) DT104 strains were found. The two large groups of DT104 strains (Felix-Callow's phage types 2 and 2c) proved to be identical in respect of integron patterns (IPs), supporting the views of those authors who consider DT104 a single clone. Strains of human and animal origin did not differ from each other in their IPs. Within the non-DT104 phage types, ampicillin-and/or streptomycin-resistant, integron-free MDR strains were also found. Based on amplicons varying between 290 and 3500 bp an IP system was suggested. The commonest amplicon sizes in non-DT104 strains were 1450 and 2050 bp. The IPs of DT104 strains and of non-DT104 strains containing an integron of 1 and 1.2 kb size were stable. In contrast, the IPs of other non-DT104 strains showed a varying degree of instability. Integron loss was frequently associated with spontaneous plasmid elimination and changes of R-type among the descendants of a given strain.Key words: Typhimurium, phage type, DT104, integron pattern, instability, PCR Since the early 1990s, the DT104 phage type of Salmonella enterica serotype Typhimurium (S. Typhimurium) has spread all over the world (Threlfall et al

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Section: Discussionmentioning

confidence: 98%

Integron content of Salmonella enterica serotype Typhimurium strains isolated in Hungary in the years 1997-1999

Gadó

Pászti

Király

et al. 2003

Acta Veterinaria Hungarica

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“…Using pulsed-field gel electrophoresis (PFGE), several studies have concluded that multidrug-resistant S. enterica Typhimurium DT104 has probably been spread clonally in European countries and the United States [6,16,30,37,40,48]. However, a recent study by Markogiannakis et al [32] also using PFGE showed that six distinct clones were present among Greek multidrug-resistant S. enterica Typhimurium DT104 isolates.…”

Section: Clonal Spread and Horizontal Transfer Of The Antibiotic Resimentioning

confidence: 99%

Molecular characterization, spread and evolution of multidrug resistance in Salmonella enterica Typhimurium DT104

2001

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-Multidrug-resistant Salmonella enterica serovar Typhimurium phage type DT104 has emerged during the last decade as a global health problem because of its involvement in diseases in animals and humans. Multidrug-resistant DT104 strains are mostly resistant to ampicillin, chloramphenicol, streptomycin, sulfonamides and tetracyclines (ACSSuT resistance type). The genes coding for such resistances are clustered on the chromosome. This paper reviews new developments in the characterization of S. enterica Typhimurium DT104, its chromosomal antibiotic resistance genes and their spread among other S. enterica Typhimurium phage types and other S. enterica serovars, the development of specific detection methods, virulence characteristics, and the evolution of multidrugresistance with regard to the emergence of quinolone resistance.Salmonella Typhimurium DT104 / multidrug resistance / Salmonella genomic island I Résumé -Caractérisation moléculaire, diffusion et évolution de la résistance multiple aux antibiotiques chez Salmonella enterica Typhimurium DT104. Le lysotype DT104 de Salmonella enterica Typhimurium présentant une résistance multiple aux antibiotiques a émergé durant la dernière décennie comme un problème mondial de santé publique qui touche à la fois humains et animaux. Les souches du lysotype DT104 sont en général résistantes à l'ampicilline, au chloramphénicol, à la streptomycine, aux sulphamides et aux tétracyclines (profil de résistance ACSSuT). Les gènes codant pour ces résistances sont groupés sur le chromosome. Cet article présente une revue des derniers dévelop-pements dans la caractérisation de S. enterica Typhimurium DT104, ses gènes chromosomiques de résis-tance et leur diffusion chez d'autres lysotypes de S. enterica Typhimurium et d'autres serotypes de S. enterica, le développement de méthodes spécifiques de détection, les caractéristiques de virulence, et l'évolution de la résistance multiple au regard de l'émergence de la résistance aux quinolones.Salmonella Typhimurium DT104 / résistance multiple / îlot génomique I Vet. Res. 32 (2001) [301][302][303][304][305][306][307][308][309][310] 301

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“…Since 1990 increasing incidence of infections in both humans and animals caused by multiresistant S. Typhimurium phage type DT104 were reported in many European countries [1,26,29] and in the USA [8]. Molecular studies have demonstrated that the resistance to β-lactams, aminoglycosides and sulfonamides are chromosomally encoded by two different integrons [9,27].…”

Section: Introductionmentioning

confidence: 99%

“…Molecular typing analyses indicated that DT104 isolates originating from different hosts and geographic origins are highly clonal [7,15,26,30]. The determination of plasmid profiles in DT104 isolates revealed that the majority of strains possess a single 60 MDa plasmid and in some strains smaller plasmids between 5 and 1 MDa as well [26,30].…”

Section: Introductionmentioning

confidence: 99%

“…The determination of plasmid profiles in DT104 isolates revealed that the majority of strains possess a single 60 MDa plasmid and in some strains smaller plasmids between 5 and 1 MDa as well [26,30]. A study in nine Danish pig herds showed, that 93 DT104 strains except one shared an identical XbaI-PFGE pattern [6] and XbaI-PFGE, IS200 profiling with PstI and ribotyping with PvuII from European DT104 isolates resulted in one common clonal type [26]. A combination of randomly amplified polymorphic DNA (RAPD) using two different 10mer primers and subsequent restriction analysis indicated also a close relationship between DT104 strains [15].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of molecular typing methods for Salmonella enterica serovar Typhimurium DT104 isolated in Germany from healthy pigs

et al. 2001

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-The discriminatory power of four different DNA based typing methods was tested for the molecular subtyping of Salmonella Typhimurium phage type DT104 isolates. German DT104 strains (n = 133) originating from slaughter pigs were analysed by plasmid profiling, and 32 of them by pulsed-field gel electrophoresis (PFGE) using the restriction enzymes XbaI, SpeI or BlnI, random amplification of polymorphic DNA (RAPD) using 13 different primers and IS200 typing. A resulting subtyping scheme was obtained which is based on the most discriminatory power of the individual methods i.e. plasmid profiling and PFGE with all three enzymes. The index of discrimination obtained by the subtyping scheme was 0.909 closely approaching the maximum value of one. Although minor differences occurred in the molecular DNA pattern of single DT104 strains, a dominating subtyping pattern was observed confirming other studies which showed, that S. Typhimurium DT104 isolates are highly clonal.

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Clonal Relationship of Salmonella enterica Serovar Typhimurium Phage Type DT104 in Germany and Austria

Cited by 45 publications

References 19 publications

Integron content of Salmonella enterica serotype Typhimurium strains isolated in Hungary in the years 1997-1999

Integron content of Salmonella enterica serotype Typhimurium strains isolated in Hungary in the years 1997-1999

Molecular characterization, spread and evolution of multidrug resistance in Salmonella enterica Typhimurium DT104

Evaluation of molecular typing methods for Salmonella enterica serovar Typhimurium DT104 isolated in Germany from healthy pigs

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