Clonality and Antibiotic Susceptibility of<i>Yersinia enterocolitica</i>Isolated from U.S. Market Weight Hogs

Bhaduri, Saumya; Wesley, Irene V.; Richards, Harry; Draughon, Ann; Wallace, Morgan

doi:10.1089/fpd.2008.0197

Cited by 32 publications

(20 citation statements)

References 19 publications

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“…The clonal character of Y. enterocolitica was obvious only in pathogenic isolates (e.g. serotype O:3), while nonpathogenic isolates of biotype 1A were more heterogeneous [56], [57]. This explanation therefore appears unlikely.…”

Section: Discussionmentioning

confidence: 97%

Unique Activity Spectrum of Colicin FY: All 110 Characterized Yersinia enterocolitica Isolates Were Colicin FY Susceptible

et al. 2013

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Colicin FY is a plasmid encoded toxin that recognizes a yersinia-specific outer membrane protein (YiuR) as a receptor molecule. We have previously shown that the activity spectrum of colicin FY comprises strains of the genus Yersinia. In this study, we analyzed the activity of colicin FY against 110 Yersinia enterocolitica isolates differing in geographical origin and source. All isolates were characterized through analysis of 16S rRNA genes, serotyping, biotyping, restriction profiling of genomic DNA, detection of virulence markers and susceptibility to antibiotics. This confirmed the broad variability of the collection, in which all 110 Y. enterocolitica isolates, representing 77 various strains, were inhibited by colicin FY. Although isolates showed variable levels of susceptibility to colicin FY, it was not associated with any strain characteristic. The universal susceptibility of Y. enterocolitica strains to colicin FY together with the absence of activity towards strains outside the Yersinia genus suggests potential therapeutic applications for colicin FY.

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Section: Discussionmentioning

confidence: 97%

Unique Activity Spectrum of Colicin FY: All 110 Characterized Yersinia enterocolitica Isolates Were Colicin FY Susceptible

et al. 2013

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“…Yersiniosis has been associated with the consumption of pork products, and pigs are thought to be the primary animal reservoir of Y. enterocolitica infectious to humans (Bhaduri et al., ). Cases have been attributed to exposure to other food vehicles such as milk products, chow mein (probably contaminated by a food handler), foods that had been in contact with contaminated water (Robins‐Browne, ) and by secondary transmission (Jones et al., ).…”

Section: Introductionmentioning

confidence: 99%

The Limitations of Pulsed‐Field Gel Electrophoresis for Analysis of Yersinia enterocolitica Isolates

Gilpin

Robson

Lin

et al. 2013

Zoonoses and Public Health

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This study describes the analysis of 432 isolates of Yersinia enterocolitica by pulsed-field gel electrophoresis (PFGE). PFGE had a high level of discrimination with biotype 1A isolates (Simpson's Diversity Index 0.997), but with the clinically important biotypes 2, 3 and 4, the discriminatory ability of PFGE was so low as to severely limit its usefulness (DI <0.6). For biotypes 2, 3 and 4, 79% or more of isolates of each biotype were of just three different PFGE profiles. Because of this, four known outbreaks of yersiniosis would not have been identified by PFGE analysis. However, a previously unrecognized potential outbreak of yersiniosis caused by biotype 4 isolates was identified on the basis of a rare PFGE genotype with spatial and temporal clustering. We conclude that PFGE has a very limited application to the genotyping of Y. enterocolitica biotypes 2, 3 and 4, and inferences based on finding indistinguishable PFGE profiles among cases or between cases and sources need to be substantiated using alternative typing tools, or strong epidemiological evidence.

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“…Y. enterocolitica is a known ß-lactamase producer and thus is resistant to ß-lactam antibiotics such as ampicillin, carbenicillin, penicillin, and first-generation cephalosporins [15-20]. In recent studies done in Switzerland, the USA, Germany, and Austria, Y. enterocolitica strains have shown high susceptibility to antimicrobials other than ß-lactams [21-24]. However, multiresistant Y. enterocolitica strains have also been reported, e.g.…”

Section: Introductionmentioning

confidence: 99%

Multilocus Variable-Number Tandem-Repeat Analysis, Pulsed-Field Gel Electrophoresis, and Antimicrobial Susceptibility Patterns in Discrimination of Sporadic and Outbreak-Related Strains of Yersinia enterocolitica

et al. 2011

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BackgroundWe assessed the potential of multilocus variable-number tandem-repeat analysis (MLVA), pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility testing for discriminating 104 sporadic and outbreak-related Yersinia enterocolitica (YE) bio/serotype 3-4/O:3 and 2/O:9 isolates. MLVA using six VNTR markers was performed in two separate multiplex PCRs, and the fluorescently labeled PCR products were accurately sized on an automated DNA sequencer.ResultsMLVA discriminated 82 sporadic YE 3-4/O:3 and 2/O:9 strains into 77 types, whereas PFGE with the restriction enzyme NotI discriminated the strains into 23 different PFGE pulsotypes. The discriminatory index for a sporadic strain was 0.862 for PFGE and 0.999 for MLVA. MLVA confirmed that a foodborne outbreak in the city of Kotka, Finland in 2003 had been caused by a multiresistant YE 4/O:3 strain that was distinctly different from those of epidemiologically unrelated strains with an identical PFGE pulsotype. The multiresistance of Y. enterocolitica strains (19% of the sporadic strains) correlated significantly (p = 0.002) with travel abroad. All of the multiresistant Y. enterocolitica strains belonged to four PFGE pulsotypes that did not contain any susceptible strains. Resistance to nalidixic acid was related to changes in codons 83 or 87 that stemmed from mutations in the gyrA gene. The conjugation experiments demonstrated that resistance to CHL, STR, and SUL was carried by a conjugative plasmid.ConclusionsMLVA using six loci had better discriminatory power than PFGE with the NotI enzyme. MLVA was also a less labor-intensive method than PFGE and the results were easier to analyze. The conjugation experiments demonstrated that a resistance plasmid can easily be transferred between Y. enterocolitica strains. Antimicrobial multiresistance of Y. enterocolitica strains was significantly associated with travel abroad.

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Clonality and Antibiotic Susceptibility ofYersinia enterocoliticaIsolated from U.S. Market Weight Hogs

Cited by 32 publications

References 19 publications

Unique Activity Spectrum of Colicin FY: All 110 Characterized Yersinia enterocolitica Isolates Were Colicin FY Susceptible

Unique Activity Spectrum of Colicin FY: All 110 Characterized Yersinia enterocolitica Isolates Were Colicin FY Susceptible

The Limitations of Pulsed‐Field Gel Electrophoresis for Analysis of Yersinia enterocolitica Isolates

Multilocus Variable-Number Tandem-Repeat Analysis, Pulsed-Field Gel Electrophoresis, and Antimicrobial Susceptibility Patterns in Discrimination of Sporadic and Outbreak-Related Strains of Yersinia enterocolitica

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