Clone and Function Verification of the OPR gene in Brassica napus Related to Linoleic Acid Synthesis

Abstract

Background Fatty acid composition and content affect rapeseed oil quality. Fatty acid synthesis-related genes in rapeseed have been studied globally by researchers. Nevertheless, rapeseed oil is mainly composed of seven different fatty acids (FA), and each fatty acid was regulated by different genes. Furthermore, different FA affect each other, which needs continuous and in-depth research to obtain more clear results in Brassica napus. Results In this paper, broad-scale miRNA expression profiles were constructed and 21 differentially expressed miRNAs were detected. GO enrichment analysis showed that most up-regulated proteins were involved in transcription factor activity and catalytic activity. KEGG pathway enrichment analysis indicated that 20 pathways involving 36 target genes were enriched, of which the bna00592 pathway may be involved in fatty acid metabolism. The results were verified using a quantitative real-time PCR (RT-qPCR) analysis, we found that the target gene of bna-miR156b > c > g was the OPR (12-oxo-phytodienoic acid reductase). Four copies of OPR gene were found, and the over-expression vectors (pCAMBIA1300-35 s-OPR and pCAMBIA1300-RNAi-OPR) were constructed to verify their functions. In T1 and T2 generation, the content of linoleic acid (LA) increased significantly in OE but deceased in OPRi. Conclusions This is the first study to provide four copies of the OPR gene that regulates LA metabolism, can be used for the molecular mechanism of LA and optimizing fatty acid profiles in oilseed for breeding programs.

Comprehensive evaluation of high-oleic rapeseed (Brassica napus) based on quality, resistance, and yield traits: A new method for rapid identification of high-oleic acid rapeseed germplasm

“…However, recent studies have shown that oleic acid is the more important essential fatty acid [ 3 ]. High oleic acid rape oil not only has high smoke point, but also protects cardiovascular health [ 4 ], which is favored by the majority of consumers.…”

Deciphering the Interaction between Coniella granati and Pomegranate Fruit Employing Transcriptomics

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Comparative Study of Bolting Adaptability between 60Co-Induced Rape and Its Original Material

“…Differentially expressed genes and metabolites in rape seedlings were screened under different light conditions by transcriptomes and metabolomes, laying a foundation for the study of the anthocyanin synthesis mechanism [23]. Most of the studies are based on the transcriptomes and metabolomes involved in the stress resistance of rape as well as yield improvement [24,25]. There is little research on the physiological taste and quality of fresh shoots.…”