Clone bank of the tobacco (Nicotiana tabacum) chloroplast genome as a set of overlapping restriction endonuclease fragments: Mapping of eleven ribosomal protein genes

Sugiura, Masahiro; Shinozaki, Kazuo; Zaita, N.; Kusuda, Mie; Kumano, Masanobu

doi:10.1016/0168-9452(86)90093-2

Cited by 201 publications

(85 citation statements)

References 20 publications

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“…To determine the binding specificity of CND41 for chloroplast DNA fragments, tobacco chloroplast DNA clones (pTBal , pTB9, pTB13, pTBl9,, pTB26, pTB28, pTX6, and pTS6;Sugiura et al, 1986) and pBR322 were digested with adequate amounts of restriction enzyme, and the individual chloroplast DNA inserts were isolated. DNA frdgments for psbA promoter regions from -200 to -100 and from -100 to -1, and a transcribed region from +1 to +100, were amplified by polymerase chain reaction using three sets of primer and Taq DNA polymerase (Takara Shuzo, Kyoto, Japan) from pTB28.…”

Section: Methodsmentioning

confidence: 99%

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A novel protein with DNA binding activity from tobacco chloroplast nucleoids.

et al. 1997

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A 41 -kD DNA binding protein with a basic pl was purified from chloroplast nucleoids in photomixotrophically cultured tobacco cells, and its amino acid sequence was determined. Using this sequence information, its cDNA (CND41) was isolated, and its nucleotide sequence was determined. The predicted amino acid sequence of CND41 has a transit peptide of 120 amino acids and a mature protein of 382 amino acids. A distinctive helix-turn-helix motif in the lysine-rich N-terminal region of the mature protein and an aspartyl protease active site motif were predicted. Expression of a series of truncated CND41 proteins in Escherichia coli indicated that the lysine-rich region is essential for DNA binding and that CND41 nonspecifically binds chloroplast DNA. Protein gel blot analyses showed CND41 mainly in cells and/or tissues containing nonphotosynthesizing, actively growing plastids. In addition, the accumulation of chloroplast transcripts in these cells and/or tissues (e.g., transcripts for Qe binding protein of photosystem II [@A] and large subunit of ribulose bisphosphate carboxylase [rbcL]) was negatively correlated with the accumulation of CND41. Analyses of cultured cells of transgenic tobacco with reduced CND41 levels showed a higher leve1 of expression of chloroplast genes compared with that of the wild type. We discuss the possible function of CND41 as a negative regulator of chloroplast gene expression.

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Section: Methodsmentioning

confidence: 99%

“…Total DNA was prepared according to the method of Sugiura et al (1986) from tissues used in the protein gel blot analysis (described above). DNAs (0.05, 0.15, and 0.5 Fg) were boiled for 10 min and then blotted onto a Hybond Nf membrane (Amersham) in a vacuum slot blot apparatus (Bio-Rad).…”

Section: Estimation Of Chloroplast Dna Copy Number Referencesmentioning

confidence: 99%

A novel protein with DNA binding activity from tobacco chloroplast nucleoids.

et al. 1997

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“…Once separated, restriction fragments were transferred to a Zetaprobe-GT nylon membrane (G/BRL) using the Southern (1975) blotting procedure. Selected cloned fragments from Pennisetum americanum (pMC; Thomas et al, 1984), Triticum aestivum (Tr; Bowman et al, 1981), Nicotiana tabacum (N; Sugiura et al, 1986), and Vigna radiata (MB; Palmer and Thompson, 1981) cpDNA recombinant libraries were used as heterologous probes for restriction fragment detection (Fig. 1).…”

Section: Methodsmentioning

confidence: 99%

Phylogenetic Relationships Among Puccinellia and Allied Genera of Poaceae as Inferred from Chloroplast DNA Restriction Site Variation

Choo

Soreng

Davis

1994

American Journal of Botany

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“…Initially, 3 mg DNA from five individuals per population was digested with six restriction enzymes (BclI, BglII, EcoRI, EcoRV, HindIII, XbaI) and hybridised with heterologous probes covering the majority of the chloroplast genome. Six petunia cpDNA probes were used, P1, P3, P4, P6, P8, P10 (details given in Sytsma and Gottlieb, 1986), plus one tobacco cpDNA probe, pTBa1 Suguira et al, 1986). Restriction digestion and hybridisation were as described in Byrne and Moran (1994), and probe plasmids were linearised and then labelled with 32 P using the random priming method.…”

Section: Plant Collectionsmentioning

confidence: 99%

Phylogeography and divergence in the chloroplast genome of Western Australian Sandalwood (Santalum spicatum)

Byrne¹,

MacDonald²,

Brand³

2003

Heredity

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Western Australian sandalwood (Santalum spicatum) is widespread throughout Western Australia across the semiarid and arid regions. The diversity and phylogeographic patterns within the chloroplast genome of S. spicatum were investigated using restriction fragment length polymorphism analysis of 23 populations. The chloroplast diversity was structured into two main clades that were geographically separated, one centred in the southern (semiarid region) and the other in the northern (arid) region. Fragmentation due to climatic instability was identified as the most likely influence on the differentiation of the lineages. The lineage in the arid region showed a greater level of differentiation than that in the southern region, suggesting a higher level of gene flow or a more recent range expansion of sandalwood in the southern region. The phylogeographic pattern in the chloroplast genome is congruent with that detected in the nuclear genome, which identified different genetic influences between the regions and also suggested a more recent expansion of sandalwood in the southern region.

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Clone bank of the tobacco (Nicotiana tabacum) chloroplast genome as a set of overlapping restriction endonuclease fragments: Mapping of eleven ribosomal protein genes

Cited by 201 publications

References 20 publications

A novel protein with DNA binding activity from tobacco chloroplast nucleoids.

A novel protein with DNA binding activity from tobacco chloroplast nucleoids.

Phylogenetic Relationships Among Puccinellia and Allied Genera of Poaceae as Inferred from Chloroplast DNA Restriction Site Variation

Phylogeography and divergence in the chloroplast genome of Western Australian Sandalwood (Santalum spicatum)

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