Clone Identification and Genetic Relationship among Candidates for Superior Trees in Rhus succedanea L. Using ISSR, AFLP, and RAPD Markers.

Hiraoka, Yuichiro; Kuramoto, Noritsugu; Okamura, Masanori; Ohira, Mineko; Taniguchi, Toru; Fujisawa, Yoshitake

doi:10.4005/jjfs.91.246

Cited by 6 publications

(16 citation statements)

References 16 publications

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“…Hiraoka et al (2009) found that more than 30 candidate trees had the same genotypes as some major traditional cultivars (Table 1); however, there were some inconsistencies between the results of Goto et al (1997) and Hiraoka et al (2009). In addition, there were some inconsistencies in the genotyping results obtained from the ISSR and AFLP markers by Hiraoka et al (2009); therefore, more robust and reproducible markers are needed for clear, unambiguous identification of these (and other) individuals.…”

mentioning

confidence: 79%

“…Total genomic DNA from each individual was extracted using a Plant Genomic DNA Mini kit (VIOGENE, Sijhih, Taipei County, Taiwan). The DNA of the candidate trees has been previously genotyped by ISSR and AFLP markers (Hiraoka et al, 2009).…”

Section: Methodsmentioning

confidence: 99%

“…Hiraoka et al (2009) suggested that the candidate trees included some traditional cultivars and were clustered weakly into two groups, based on un-weighted pair-group method with arithmetic means (UPGMA) analysis and principal component analysis (PCA); they also found no significant relationship between the genetic similarity and geographic distance between the sources of the individuals. These results strongly suggest that both the artificial movement of individuals and natural mating have occurred in the past around western Japan (Hiraoka et al, 2009). However, the microsatellite-based study presented here was undertaken because microsatellites could provide better indications of the relatedness between individuals than dominant markers, due to their polymorphism and codominance.…”

Section: Traditional Cultivarmentioning

confidence: 99%

“…Individuals identified as traditional cultivar (Hiraoka et al, 2009) Other Goto et al (1997) and the ISSR, AFLP and RAPD analyses performed by Hiraoka et al (2009) were to examine genetic similarities among the candidate trees selected for the R. succedanea breeding program. Hiraoka et al (2009) suggested that the candidate trees included some traditional cultivars and were clustered weakly into two groups, based on un-weighted pair-group method with arithmetic means (UPGMA) analysis and principal component analysis (PCA); they also found no significant relationship between the genetic similarity and geographic distance between the sources of the individuals.…”

Section: Traditional Cultivarmentioning

confidence: 99%

“…the individual 'Ariake3', which was identified as belonging to the traditional cultivar 'Ikichi' (Table 1) by Hiraoka et al (2009), was digested with NheI, HhaI and AluI. Double-stranded SNX linkers (Hamilton et al, 1999) were ligated at both ends of the digested fragments using T4 DNA ligase.…”

Section: Microsatellite Development and Analysismentioning

confidence: 99%

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Development and Characterization of Microsatellites, Clone Identification, and Determination of Genetic Relationships among Rhus succedanea L. Individuals

Hiraoka

Watanabe

2010

J. Japan. Soc. Hort. Sci.

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To assist in the breeding and identification of Rhus succedanea L., which is cultivated in Japan to produce wax from its fruit, 12 microsatellite markers were developed from 96 clones sequenced from a genomic library generated from the traditional cultivar 'Ikichi'. Using these markers, 50 genotypes were identified in 82 individuals (designated "the western group") representing traditional cultivars and associated materials that have been selected from populations in western Japan and conserved for use in a breeding program. In a further analysis, of the 82 previously mentioned individuals and others from Chiba and Okinawa populations, 88 different genotypes were detected, with numbers of alleles per locus ranging from 3 to 19 and expected heterozygosity ranging from 0.22 to 0.85. Each traditional cultivar and old individual in the western group was closely genetically related to other trees in the group. In addition, according to Principal Coordinate Analysis, using Dice similarity coefficients based on the 88 genotypes, and pairwise F ST values, the western group and the Chiba populations were considerably more closely related to each other than to the Okinawa populations. The implications of the findings for the future breeding of R. succedanea in Japan are discussed.

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confidence: 79%

Section: Methodsmentioning

confidence: 99%

Section: Traditional Cultivarmentioning

confidence: 99%

Section: Traditional Cultivarmentioning

confidence: 99%

Section: Microsatellite Development and Analysismentioning

confidence: 99%

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Development and Characterization of Microsatellites, Clone Identification, and Determination of Genetic Relationships among Rhus succedanea L. Individuals

Hiraoka

Watanabe

2010

J. Japan. Soc. Hort. Sci.

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Estimation of genetic data and breeding values of traits related to wax production inRhus succedaneaL. clones using the REML/BLUP method

Hiraoka

Kuramoto²,

Ohira³

et al. 2011

Journal of Forest Research

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Rhus succedanea L. is cultivated in Japan for the wax that can be extracted from its fruits. We determined genetic data for specific traits associated with wax production (fruit number per cluster, FN, fruit weight, FW, number of clusters per area, CN, and wax content, WC) in five traditional cultivars and 13 newer clones. Data were collected over 4 years and alternate bearing was observed: 2001 and 2003 were years with high yields; in 2002 and 2004 yields were poor. The restricted maximum likelihood method was used to calculate (co)variance components for analysis. Broad sense heritability was estimated and ranged from low (FN and CN) to high (FW and WC) for the traits examined. There were positive genetic correlations between FW and WC and negative ones between FN and FW throughout the 4 years. Genetic correlations between CN and the other traits were positive in good crop years and negative in bad crop years. For each assessed clone, the four traits in the 4 years were evaluated using best linear unbiased predictors (BLUP) of the clonal breeding values.The BLUP scores for both FW and WC exhibited positive correlations between pairs of years, whereas there were positive BLUP correlations for CN when the high yield and low yield years were examined separately. The traditional cultivars and the newer clones were also characterized. Some of the clones were better than the traditional cultivars with regard to wax yield and reliability of production. The trait characteristics and the future breeding are discussed.

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Biotechnologies for the Management of Genetic Resources for Food and Agriculture

Lidder

Sonnino

2012

Advances in Genetics

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Clone Identification and Genetic Relationship among Candidates for Superior Trees in Rhus succedanea L. Using ISSR, AFLP, and RAPD Markers.

Cited by 6 publications

References 16 publications

Development and Characterization of Microsatellites, Clone Identification, and Determination of Genetic Relationships among Rhus succedanea L. Individuals

Development and Characterization of Microsatellites, Clone Identification, and Determination of Genetic Relationships among Rhus succedanea L. Individuals

Estimation of genetic data and breeding values of traits related to wax production inRhus succedaneaL. clones using the REML/BLUP method

Biotechnologies for the Management of Genetic Resources for Food and Agriculture

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