Cloners quick on the uptake

Attwell, David; Bouvier, Muriel

doi:10.1016/0960-9822(92)90024-5

Cited by 15 publications

(11 citation statements)

References 11 publications

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“…Besides, sodium: neurotransmitter symporter (SNF) family genes (such as SLC6A1 , SLC6A6 , SLC6A8 , SLC6A11 , SLC6A12, and SLC6A13 ) were also expanded in yellow drum in contrast to large yellow croaker. Those genes are essential for the release, re‐uptaking, and recycling of neurotransmitters at synapses (Attwell & Bouvier, ). SLC6A1 , SLC6A11, and SLC6A13 , widely distributed in brain, encode sodium‐dependent transporters that uptake gamma‐aminobutyric acid (GABA) (Zhou et al, ).…”

Section: Resultsmentioning

confidence: 99%

Near‐complete genome assembly and annotation of the yellow drum (Nibea albiflora) provide insights into population and evolutionary characteristics of this species

Han

Zhu

et al. 2018

Ecology and Evolution

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Yellow drum (Nibea albiflora) is an important fish species in capture fishery and aquaculture in East Asia. We herein report the first and near‐complete genome assembly of an ultra‐homologous gynogenic female yellow drum using Illumina short sequencing reads. In summary, a total of 154.2 Gb of raw reads were generated via whole‐genome sequencing and were assembled to 565.3 Mb genome with a contig N50 size of 50.3 kb and scaffold N50 size of 2.2 Mb (BUSCO completeness of 97.7%), accounting for 97.3%–98.6% of the estimated genome size of this fish. We further identified 22,448 genes using combined methods of ab initio prediction, RNAseq annotation, and protein homology searching, of which 21,614 (96.3%) were functionally annotated in NCBI nr, trEMBL, SwissProt, and KOG databases. We also investigated the nucleotide diversity (around 1/390) of aquacultured individuals and found the genetic diversity of the aquacultured population decreased due to inbreeding. Evolutionary analyses illustrated significantly expanded and extracted gene families, such as myosin and sodium: neurotransmitter symporter (SNF), could help explain swimming motility of yellow drum. The presented genome will be an important resource for future studies on population genetics, conservation, understanding of evolutionary history and genetic breeding of the yellow drum and other Nibea species.

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Section: Resultsmentioning

confidence: 99%

Near‐complete genome assembly and annotation of the yellow drum (Nibea albiflora) provide insights into population and evolutionary characteristics of this species

Han

Zhu

et al. 2018

Ecology and Evolution

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“…It is possible that proline oxidase plays a significant role in the formation of some neural glutamate pools (34). The possible role ofproline as a neurotransmitter has recently become the focus of considerable attention, with the cloning of a proline transporter molecule (35,36). This is expressed in or near glutamatergic neurons, whereas the relevant enzymes for synthesis of glutamate from proline are found in glial cells, not neurons (reviewed in ref.…”

Section: Discussionmentioning

confidence: 99%

The sluggish-A gene of Drosophila melanogaster is expressed in the nervous system and encodes proline oxidase, a mitochondrial enzyme involved in glutamate biosynthesis.

Hayward

Delaney

Campbell

et al. 1993

Proc. Natl. Acad. Sci. U.S.A.

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“…This uncertainty has led to the assumption that the glycine concentration in the synaptic cleft is close to the values (1-10 M) measured in the CSF (Westergren et al, 1994), which corresponds to a saturating level for the glycine site of most NMDARs. In contrast, based on the thermodynamics of the transporter, which predicts a limiting external glycine concentration in the 100 nM range, it has been suggested that an active uptake of glycine by transporters might be sufficient to reduce below saturation the glycine concentration in the synaptic cleft (Ascher, 1990;Attwell and Bouvier, 1992;Attwell et al, 1993), thereby allowing the variations in the local glycine concentration to become a signal acting on NMDARs. This hypothesis has two major kinetic limitations.…”

Section: Abstract: Glutamate Receptor; Nmda; Coagonist; D-serine; Samentioning

confidence: 99%

“…The theoretical limit for the decrease of the external glycine concentration is imposed by the thermodynamics of the transport, as defined by the relation (Attwell and Bouvier, 1992;Attwell et al, 1993): noted that under our experimental condition, the equilibrium is never obtained, because a steady-state inward flux is present between the bath and the oocyte cytoplasm.…”

Section: Control Of the [Gly] M By Glyt1bmentioning

confidence: 99%

Control of NMDA Receptor Activation by a Glycine Transporter Co-Expressed inXenopusOocytes

1997

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We present evidence that membrane transporters can control the membrane receptor's agonist concentration in restricted extracellular spaces of a biological model. The model is constructed by co-expressing glycine/Na/Cl cotransporters (GLYT1b) and NMDA receptors (NMDARs) (composed of the subunits NR1 and NR2A or NR2B) in Xenopus oocytes. We use the high-affinity glycine site of the NMDARs as a sensor of the actual juxtamembrane glycine concentration. We show that glycine uptake by GLYT1b dramatically reduces NMDAR currents by reducing the glycine concentration in extracellular spaces in which diffusion is restricted. This effect appears only in oocytes in which GLYT1b and NMDAR are co-expressed. It is Na ϩ -and voltage-dependent, and is abolished when Na ϩ is replaced by Li ϩ and when glycine is replaced by D-serine (a coagonist of the NMDAR that is not transported by GLYT1b). These results demonstrate the ability of the GLYT transporter to reduce glycine concentration at the level of NMDARs in restricted diffusion spaces. This observation could account for a prevalent role of membrane transporters in the modulation of synapse transmission in the CNS. From a more general point of view, our results draw attention to possible significant discrepancies between local concentrations at the level of substrate targets in biological membranes and their concentration in the bulk solution when membrane transporters are present.

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Cloners quick on the uptake

Cited by 15 publications

References 11 publications

Near‐complete genome assembly and annotation of the yellow drum (Nibea albiflora) provide insights into population and evolutionary characteristics of this species

Near‐complete genome assembly and annotation of the yellow drum (Nibea albiflora) provide insights into population and evolutionary characteristics of this species

The sluggish-A gene of Drosophila melanogaster is expressed in the nervous system and encodes proline oxidase, a mitochondrial enzyme involved in glutamate biosynthesis.

Control of NMDA Receptor Activation by a Glycine Transporter Co-Expressed inXenopusOocytes

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