Cloning and characterization of a novel 2-ketoisovalerate reductase from the beauvericin producer Fusarium proliferatum LF061

Zhang, Tao; Jia, Xiaoxu; Zhuo, Ying; Liu, Mei; Gao, Hong; Liu, Jintao; Zhang, Lixin

doi:10.1186/1472-6750-12-55

Cited by 15 publications

(29 citation statements)

References 32 publications

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“…Recently, BEA-producing species have also been identified by cloning and characterization of the respective biosynthetic genes in B. bassiana [36] and F. proliferatum . Unfortunately, only a few reports are available on the structure of the gene cluster in other BEA producers [37]. …”

Section: Resultsmentioning

confidence: 99%

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Sequence Divergence of the Enniatin Synthase Gene in Relation to Production of Beauvericin and Enniatins in Fusarium Species

Stępień

Waśkiewicz

2013

Toxins

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Beauvericin (BEA) and enniatins (ENNs) are cyclic peptide mycotoxins produced by a wide range of fungal species, including pathogenic Fusaria. Amounts of BEA and ENNs were quantified in individual rice cultures of 58 Fusarium strains belonging to 20 species, originating from different host plant species and different geographical localities. The species identification of all strains was done on the basis of the tef-1α gene sequence. The main aim of this study was to analyze the variability of the esyn1 gene encoding the enniatin synthase, the essential enzyme of this metabolic pathway, among the BEA- and ENNs-producing genotypes. The phylogenetic analysis based on the partial sequence of the esyn1 gene clearly discriminates species producing exclusively BEA from those synthesizing mainly enniatin analogues.

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Section: Resultsmentioning

confidence: 99%

“…Consequently, significant sequence homologies to certain Fusarium enzymes were found [36]. Recently, the homologous cluster from F. proliferatum was sequenced, and the gene encoding ketoisovalerate reductase—an enzyme controlling the initial step of the pathway—was characterized [37]. …”

Section: Introductionmentioning

confidence: 99%

Sequence Divergence of the Enniatin Synthase Gene in Relation to Production of Beauvericin and Enniatins in Fusarium Species

Stępień

Waśkiewicz

2013

Toxins

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“…The BEA biosynthetic pathway was recently characterized by Zhang et al . (, ) in Fusarium proliferatum . BEA and ENs share the same biosynthetic pathway (Kleinkauf ; Peeters et al .…”

Section: Discussionmentioning

confidence: 99%

Transcriptional regulation of enniatins production by Fusarium avenaceum

et al. 2013

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Aims: The aim of this study was to analyse the transcriptional regulation of enniatins (ENs) production in Fusarium avenaceum. Methods and Results: We develop a new method to quantify ENs in FDM agar medium. We performed an LC/MS/MS analysis to evaluate enniatin A, A1, B, B1 and B4 production by seven F. avenaceum strains and, in a timecourse experiment, by ITEM 3404 to analyse the transcriptional regulation of the esyn1 gene. The expression profile, achieved by Real time reverse transcriptase assay, showed an activation of gene transcription at the seventh day of incubation, corresponding to the higher increase of total ENs production. Enniatin B was the most abundant ENs analogues, representing the 90% of total ENs. The relative percentage of ENs remained unaltered during the experiment. Conclusions: We reported a transcriptional regulation of esyn1 responsible for the modulation of ENs biosynthesis. Significance and Impact of the Study: Enniatins are cyclic depsipeptides metabolites with a wide range of biological activities. They are also widespread contaminants in grains and cereals due to infection by enniatin-producing Fusarium species. This is the first article describing the transcriptional regulation of esyn1 gene that modulates ENs production in Fusarium avenaceum and provides new knowledge about the molecular mechanism underlying the biosynthesis of these important fungal metabolites in this toxigenic fungal species.

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“…Hydroxyisovalerate (Hiv) is a common 2‐hydroxycarboxylic acid ingredient of depsipeptides including beauvericin. In fungi, D‐Hiv is formed by the highly specific reduction reaction of 2‐ketoisovalerate (2‐Kiv) by 2‐ketoisovalerate reductase (KIVR) (Lee et al ., ; Zhang et al ., ). Beauvericin production is inhibited in a KIVR knockout B. bassiana , suggesting a critical role of KIVR in the beauvericin biosynthesis (Xu et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Calmodulin‐mediated suppression of 2‐ketoisovalerate reductase in Beauveria bassiana beauvericin biosynthetic pathway

Kim

Yoon

et al. 2016

Environmental Microbiology

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Ketoisovalerate reductase (KIVR, E.C. 1.2.7.7) mediates the specific reduction of 2-ketoisovalerate (2-Kiv) to d-hydroxyisovalerate (d-Hiv), a precursor for beauvericin biosynthesis. Beauvericin, a famous mycotoxin produced by many fungi, is a cyclooligomer depsipeptide, which has insecticidal, antimicrobial, antiviral, and cytotoxic activities. In this report, we demonstrated that Beauveria bassiana 2-ketoisovalerate reductase (BbKIVR) acts as a typical KIVR enzyme in the entomopathogenic fungus B. bassiana. In addition, we found that BbKIVR interacts with calmodulin (CaM) in vitro and in vivo. The functional role of CaM-binding to BbKIVR was to negatively regulate the BbKIVR activity in B. bassiana. Environmental stimuli such as light and salt stress suppressed BbKIVR activity in B. bassiana. Interestingly, this negative effect of BbKIVR activity by light and salt stress was recovered by CaM inhibitors, suggesting that the inhibitory mechanism is mediated through stimulation of CaM activity. Therefore, this work suggests that BbKIVR plays an important role in the beauvericin biosynthetic pathway mediated by environmental stimuli such as light and salt stress via the CaM signaling pathway.

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Cloning and characterization of a novel 2-ketoisovalerate reductase from the beauvericin producer Fusarium proliferatum LF061

Cited by 15 publications

References 32 publications

Sequence Divergence of the Enniatin Synthase Gene in Relation to Production of Beauvericin and Enniatins in Fusarium Species

Sequence Divergence of the Enniatin Synthase Gene in Relation to Production of Beauvericin and Enniatins in Fusarium Species

Transcriptional regulation of enniatins production by Fusarium avenaceum

Calmodulin‐mediated suppression of 2‐ketoisovalerate reductase in Beauveria bassiana beauvericin biosynthetic pathway

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