Cloning and characterization of a gene involved in phytoene synthesis from tomato

Ray, John A.; Moureau, P.; Bird, Colin R.; Bird, Alison; Grierson, Don; Maunders, Martin J.; Truesdale, Mark R.; Bramley, Peter M.; Schuch, Wolfgang

doi:10.1007/bf00023387

Cited by 69 publications

(40 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2A) was predicted to be the N-terminal residues 1-52. This result is consistent with previous results in Arabidopsis (Scolnik & Bartley 1994), tomato (Ray et al 1992), and sunflower (Salvini et al 2005) which are 1-70, 1-62, and 1-67 residues, respectively.…”

Section: Discussionsupporting

confidence: 93%

cDNA cloning of the phytoene synthase (PSY) and expression analysis of PSY and carotenoid cleavage dioxygenase genes in Osmanthus fragrans

Han

Dong

et al. 2013

Biologia

View full text Add to dashboard Cite

In the carotenoid biosynthesis pathway, phytoene synthase (PSY) catalyzes the dimerization of two molecules of geranylgeranyl pyrophosphate (GGPP) to phytoene and has been shown to be a rate-limiting enzyme for the synthesis of carotenoids. In this study, a PCR approach was used to isolate a phytoene synthase cDNA from flower of Osmanthus fragrans. It contained a 1314 bp CDS. The predicted protein (49.4 kDa) displayed a sequence of 438 amino acid residues. Phylogenetic analysis demonstrated that the O. fragrans PSY gene (OfPSY) clustered with Gardenia jasminoides, Coffea canephora, Lycium barbarum and Mespilus germanica. Moreover, the OfPSY sequence relates closely with other PSY sequences of higher plants. We detected flower's carotenoid content and analysed OfPSY and carotenoid cleavage dioxygenase genes (OfCCD1 and OfCCD4) in two cultivars of O. fragrans with varied coloration, including O. fragrans "Yingui" (light yellow) and "Dangui" (orange) at different floral stages. We observed that "Dangui" can accumulate carotenoids when OfPSY transcript was already constant, and this showed that the OfPSY does not play a role in the accumulation of total carotenoid in this cultivar. However, the transcripts of OfCCD4 in "Yingui" flower petals were clearly higher than that in "Dangui". This was consistent with the ionone content in "Yingui" flower petals, which was significantly higher than that in "Dangui". This response could contribute to explain the different accumulation of carotenoids in petals of the 2 cultivars.

show abstract

Section: Discussionsupporting

confidence: 93%

cDNA cloning of the phytoene synthase (PSY) and expression analysis of PSY and carotenoid cleavage dioxygenase genes in Osmanthus fragrans

Han

Dong

et al. 2013

Biologia

View full text Add to dashboard Cite

show abstract

“…cDNA clones of two carotenoid genes from tomato have been characterized recently. A ripening-enhanced mRNA, represented by the cDNA pTOM5 (Maunders et al, 1987;Ray et al, 1992), has been shown to encode phytoene synthase (Bird et al, 1991;Bramley et al, 1992). This has been confirmed independently by Bartley and co-workers (1992), who have named the gene Psyl.…”

supporting

confidence: 53%

Carotenoid Biosynthesis during Tomato Fruit Development (Evidence for Tissue-Specific Gene Expression)

et al. 1994

View full text Add to dashboard Cite

“…To demonstrate a causal relationship between fruit ripening defects of transgenic lines and the impairment of active DNA demethylation, the expression of CNR (21), RIPENING INHIBITOR (RIN) (24), NOR (25), and PHYTOENE SYNTHASE 1 (PSY1) (26,27) genes was assessed in RNAi transgenic plants. These genes were selected among others because they are necessary for the overall ripening process (CNR, RIN, NOR), or specifically govern carotenoid accumulation (PSY1), an important quality trait of mature tomato fruit.…”

Section: Fruit-ripening Defects Are Correlated With the Repression Anmentioning

confidence: 99%

A DEMETER-like DNA demethylase governs tomato fruit ripening

Liu

How‐Kit

Stammitti

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

282

288

View full text Add to dashboard Cite

In plants, genomic DNA methylation which contributes to development and stress responses can be actively removed by DEMETER-like DNA demethylases (DMLs). Indeed, in Arabidopsis DMLs are important for maternal imprinting and endosperm demethylation, but only a few studies demonstrate the developmental roles of active DNA demethylation conclusively in this plant. Here, we show a direct cause and effect relationship between active DNA demethylation mainly mediated by the tomato DML, SlDML2, and fruit ripeningan important developmental process unique to plants. RNAi SlDML2 knockdown results in ripening inhibition via hypermethylation and repression of the expression of genes encoding ripening transcription factors and rate-limiting enzymes of key biochemical processes such as carotenoid synthesis. Our data demonstrate that active DNA demethylation is central to the control of ripening in tomato.active DNA demethylation | DNA glycosylase lyase | epigenetic | tomato | fruit ripening G enomic DNA methylation is a major epigenetic mark that is instrumental to many aspects of chromatin function, including gene expression, transposon silencing, or DNA recombination (1-4). In plants, DNA methylation can occur at cytosine both in symmetrical (CG or CHG) and nonsymmetrical (CHH) contexts and is controlled by three classes of DNA methyltransferases, namely, the DNA Methyltransferase 1, Chromomethylases, and the Domain Rearranged Methyltransferases (5-7). Indeed, in all organisms, cytosine methylation can be passively lost after DNA replication in the absence of methyltransferase activity (1). However, plants can also actively demethylate DNA via the action of DNA GlycosylaseLyases, the so-called DEMETER-Like DNA demethylases (DMLs), that remove methylated cytosine, which is then replaced by a nonmethylated cytosine (8

show abstract

Cloning and characterization of a gene involved in phytoene synthesis from tomato

Cited by 69 publications

References 9 publications

cDNA cloning of the phytoene synthase (PSY) and expression analysis of PSY and carotenoid cleavage dioxygenase genes in Osmanthus fragrans

cDNA cloning of the phytoene synthase (PSY) and expression analysis of PSY and carotenoid cleavage dioxygenase genes in Osmanthus fragrans

Carotenoid Biosynthesis during Tomato Fruit Development (Evidence for Tissue-Specific Gene Expression)

A DEMETER-like DNA demethylase governs tomato fruit ripening

Contact Info

Product

Resources

About