Cloning and characterization of a novel C-type lectin from Zhikong scallop Chlamys farreri

Wang, Hao; Song, Linsheng; Li, Chenghua; Zhao, Jianmin; Zhang, Huan; Ni, Duojiao; Xu, Wei

doi:10.1016/j.molimm.2006.04.015

Cited by 138 publications

(93 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A growing number of CTLs have recently been discovered from marine invertebrates, especially crustaceans and molluscs, including those from F. chinensis [16,21,46,47], L. vannamei [15,19,45,48], Penaeus monodon [49,50], C. farreri [18,23,24,26], A. irradians [22,25] and Crassostrea gigas [51], Crassostrea virginica [52], and Haliotis discus discus [17]. Relatively little information is available on CTLs in echinoderms, and a handful of sea cucumber CTLs have been reported, such as MBL-C (Cucumaria japonica) [53], SJL-I (S. japonicus) [35,36], MBL-AJ and AJCTL (A. japonicus) [37,38].…”

Section: Discussionmentioning

confidence: 99%

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Wei

Liu

Yang

et al. 2015

Fish & Shellfish Immunology

View full text Add to dashboard Cite

a b s t r a c t C-type lectin is one important pattern recognition receptor (PRR) that plays crucial roles in multiple immune responses. A C-type lectin from sea cucumber Apostichopus japonicus (AjCTL-1) was characterized in the present study. The amino acid sequence of AjCTL-1 shared high similarities with other Ctype lectins from invertebrates and vertebrates. The C-type lectin domain (CTLD) of AjCTL-1 contained a Ca 2þ -binding site 2 and four conserved cysteine residues. AjCTL-1 mRNA expression patterns in tissues and after bacterial challenge were then analysed. Quantitative PCR revealed that AjCTL-1 mRNA was widely expressed in the tested tissues of healthy sea cucumber. The highest expression level occurred in gonad followed by body wall, coelomocytes, tentacle, intestinum and longitudinal muscle, and the lowest expression level was in respiratory tree. AjCTL-1 mRNA expression in coelomocytes was significantly induced by gram-negative Listonella anguillarum and gram-positive Micrococcus luteus, with different upregulation patterns post-challenge. Recombinant AjCTL-1 exhibited the ability to bind peptidoglycan directly, agglutinate M. luteus, Staphylococcus aureus and Escherichia coli, in a Ca 2þ -dependant manner, and enhance the phagocytosis of coelomocytes against E. coli in vitro. The results indicated that AjCTL-1 could act as a PRR in Apostichopus japonicus and had critical roles in non-self recognition and bacterial clearance against invading microbes.

show abstract

Section: Discussionmentioning

confidence: 99%

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Wei

Liu

Yang

et al. 2015

Fish & Shellfish Immunology

View full text Add to dashboard Cite

show abstract

“…Lectin, the pattern recognition protein (PRP) recognizing and binding to terminal sugars on glycoproteins and glycolipids, is one of the major components in these host-defence systems [2]. It has been isolated and characterized from viruses [3], bacteria [4], fungi [5,6], plants [7,8], and animals [2,9,10]. Among the lectin of invertebrates origin, sialic acid binding lectins (SABL) show attractive attention for their potentially roles in glycobiology or cancer research such as detection, localization, and isolation of sialoglycoconjugates [11].…”

Section: Introductionmentioning

confidence: 99%

Cloning and characterization of a sialic acid binding lectins (SABL) from Manila clam Venerupis philippinarum

Zhao

et al. 2011

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…In recent years, many C-type lectins have been characterized in various invertebrates, such as insects, tunicates, crustaceans and bivalves [11][12][13][14]. In bivalves, lectins have been identified in haemolymph/haemocytes and were suggested to be implicated in defense responses or pathogen uptake.…”

Section: Introductionmentioning

confidence: 99%

“…In bivalves, lectins have been identified in haemolymph/haemocytes and were suggested to be implicated in defense responses or pathogen uptake. For example, five C-type lectins from Chlamys farreri (CfLec-1-CfLec-5) and seven C-type lectins from bay scallop Argopecten irradians (AiCTL-1-AiCTL-7) have been characterized, and most of them were proved to be involved in the immune responses against certain Gram-positive or Gram-negative bacteria [13,[15][16][17][18][19]. In addition, a novel putative C-type lectin identified from Crassostrea virginica was detected in the mucocytes lining the epithelium of the digestive gland and the pallial organs, and was involved in particle capture and in oyster mucosal immunity [20].…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning and expression of a C-type lectin gene from Venerupis philippinarum

Chen

Zhao

2013

Mol Biol Rep

View full text Add to dashboard Cite

C-type lectins have been demonstrated to play important roles in invertebrate innate immunity by mediating the recognition of pathogens and clearing the microinvaders. In the present study, a C-type lectin gene (denoted as VpCTL) was identified from Venerupis philippinarum by expressed sequence tag and rapid amplification of cDNA ends approaches. The full-length cDNA of VpCTL consists of 904 nucleotides with an open-reading frame of 456 bp encoding a peptide of 151 amino acids. The deduced amino acid sequence of VpCTL shared high similarity with C-type lectins from other species. The C-type lectin domain and the characteristic EPN and WND motifs were found in VpCTL. The VpCTL mRNA was dominantly expressed in the haemocytes of the V. philippinarum. After Listonella anguillarum challenge, the temporal expression of VpCTL mRNA in haemocytes was increased by 97-and 84-fold at 48 and 96 h, respectively. With high expression level in haemocytes and hepatopancreas, and the up-regulated expression in haemocytes indicted that VpCTL was perhaps involved in the immune responses to L. anguillarum challenge.

show abstract

Cloning and characterization of a novel C-type lectin from Zhikong scallop Chlamys farreri

Cited by 138 publications

References 34 publications

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Critical roles of sea cucumber C-type lectin in non-self recognition and bacterial clearance

Cloning and characterization of a sialic acid binding lectins (SABL) from Manila clam Venerupis philippinarum

Molecular cloning and expression of a C-type lectin gene from Venerupis philippinarum

Contact Info

Product

Resources

About