Cloning and characterization of a plasminogen-binding enolase from the saliva of the argasid tick Ornithodoros moubata

Díaz-Martín, Verónica; Manzano-Román, Raúl; Oleaga, Ana; Encinas-Grandes, Antonio; Pérez–Sánchez, Ricardo

doi:10.1016/j.vetpar.2012.09.019

Cited by 45 publications

(43 citation statements)

References 36 publications

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“…Ixodegrins identified in Ixodes pacificus and I. scapularis are integrin antagonists that display sequence similarity to variabilin (Francischetti et al, 2005b; Francischetti, 2010). In addition, ticks have evolved strategies to disaggregate platelet aggregates, either by displacement of fibrinogen from its receptor by competitive binding, e.g., savignygrin (Mans et al, 2002c), or by fibrinolysis (Decrem et al, 2009; Anisuzzaman et al, 2011b; Diaz-Martin et al, 2013a). …”

Section: Tick Salivamentioning

confidence: 99%

The Essential Role of Tick Salivary Glands and Saliva in Tick Feeding and Pathogen Transmission

Šimo

Kazimírová

Richardson

et al. 2017

Front. Cell. Infect. Microbiol.

230

283

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As long-term pool feeders, ticks have developed myriad strategies to remain discreetly but solidly attached to their hosts for the duration of their blood meal. The critical biological material that dampens host defenses and facilitates the flow of blood—thus assuring adequate feeding—is tick saliva. Saliva exhibits cytolytic, vasodilator, anticoagulant, anti-inflammatory, and immunosuppressive activity. This essential fluid is secreted by the salivary glands, which also mediate several other biological functions, including secretion of cement and hygroscopic components, as well as the watery component of blood as regards hard ticks. When salivary glands are invaded by tick-borne pathogens, pathogens may be transmitted via saliva, which is injected alternately with blood uptake during the tick bite. Both salivary glands and saliva thus play a key role in transmission of pathogenic microorganisms to vertebrate hosts. During their long co-evolution with ticks and vertebrate hosts, microorganisms have indeed developed various strategies to exploit tick salivary molecules to ensure both acquisition by ticks and transmission, local infection and systemic dissemination within the vertebrate host.

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Section: Tick Salivamentioning

confidence: 99%

The Essential Role of Tick Salivary Glands and Saliva in Tick Feeding and Pathogen Transmission

Šimo

Kazimírová

Richardson

et al. 2017

Front. Cell. Infect. Microbiol.

230

283

View full text Add to dashboard Cite

show abstract

“…For example enolase, a glycoolytic enzyme may act as plasminogen activator (Díaz-Martín et al 2013a). An interesting phenomenon in this regard, is the proteomic detection of high numbers of housekeeping proteins in tick salivary secretions, even though the majority do not have secretory signals (Díaz-Martín et al 2013b;Oliveira et al 2013;Tirloni et al 2015Tirloni et al , 2014.…”

Section: Moonlighting Of Housekeeping Proteins At the Tick-host Intermentioning

confidence: 99%

Ancestral reconstruction of tick lineages

Mans

Castro

Pienaar

et al. 2016

Ticks and Tick-borne Diseases

101

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“…Others have reported proteins in saliva of replete fed R. sanguineus (35) and three and five day fed Dermacentor andersoni (36). A related study reported proteins in Ornithodoros moubata (soft tick) identified from saliva collected after four months from feeding (37). Most recently, the saliva proteomes of unfed I. scapularis and A. americanum exposed to different hosts have been identified (38).…”

Section: Introductionmentioning

confidence: 99%

Time-resolved proteomic profile ofAmblyomma americanumtick saliva during feeding

Kim

Tirloni

Pinto

et al. 2019

Preprint

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24Amblyomma americanum ticks transmit more than a third of human tick-borne disease 25 (TBD) agents in the United States. Tick saliva proteins are critical to success of ticks as vectors 26 of TBD agents, and thus might serve as targets in tick antigen-based vaccines to prevent TBD 27 infections. We describe a systems biology approach to identify, by LC-MS/MS, saliva proteins 28 (tick=1182, rabbit=335) that A. americanum ticks likely inject into the host every 24 h during the 29 first 8 days of feeding, and towards the end of feeding using two different sample preparation 30 approaches (in-gel and in-solution). The in-gel approach determined molecular identification of 31 predominant protein bands in tick saliva, and the in-solution added depth to discovery of 32 proteins. Searching against entries in GenBank grouped tick and rabbit proteins in this study into 33 27 and 25 functional categories. Aside from housekeeping-like proteins, majority of tick saliva 34 proteins belong to the tick-specific (no homology to non-tick organisms: 32%), protease 35 inhibitors (13%), proteases (8%), glycine-rich proteins (6%) and lipocalins (4%) categories.36 Global secretion dynamics analysis suggests that majority (74%) of proteins in this study are 37 associated with regulating initial tick feeding functions and transmission of pathogens as they are 38 secreted within 24-48 h of tick attachment. Comparative analysis of the A. americanum tick 39 saliva proteome to five other tick saliva proteomes identified 284 conserved tick saliva proteins:40 we speculate that these regulate critical tick feeding functions and might serve as tick vaccine 41 antigens. We discuss our findings in the context of understanding A. americanum tick feeding 42 physiology as a means through which we can find effective targets for a vaccine against tick 43 feeding. 44 45 3 46 Author Summary 47The lone star tick, Amblyomma americanum, is a medically important species in US that 48 transmits 5 of the 16 reported tick-borne disease agents. Most recently, bites of this tick were 49 associated with red meat allergies in humans. Vaccination of animals against tick feeding has 50 been shown to be a sustainable and effective alternative to current acaricide based tick control 51 method which has several limitations. The pre-requisite to tick vaccine development is to 52 understand the molecular basis of tick feeding physiology. Toward this goal, this study has 53 identified proteins that A. americanum ticks inject into the host at different phases of its feeding 54 cycle. This data set has identified proteins that A. americanum inject into the host within 24-48 h 55 of feeding before it starts to transmit pathogens. Of high importance, we identified 284 proteins 56 that are present in saliva of other tick species, which we suspect regulate important role(s) in tick 57 feeding success and might represent rich source target antigens for a tick vaccine. Overall, this 58 study provides a foundation to understand the molecular mechanisms regulating tick feeding 59 p...

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Cloning and characterization of a plasminogen-binding enolase from the saliva of the argasid tick Ornithodoros moubata

Cited by 45 publications

References 36 publications

The Essential Role of Tick Salivary Glands and Saliva in Tick Feeding and Pathogen Transmission

The Essential Role of Tick Salivary Glands and Saliva in Tick Feeding and Pathogen Transmission

Ancestral reconstruction of tick lineages

Time-resolved proteomic profile ofAmblyomma americanumtick saliva during feeding

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