Cloning and characterization of a gene encoding flagellin of <i>Listeria monocytogenes</i>

Dons, Lone; Rasmussen, Ole F.; Olsen, John Elmerdahl

doi:10.1111/j.1365-2958.1992.tb01751.x

Cited by 81 publications

(79 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Strains identified as belonging in Division II were serotyped by using primers designed from the flaA gene, which encodes the L. monocytogenes flagellin protein (Table 1) (10,25). Sequencing primers were designed from regions of the flaA gene that are conserved between serotypes 4b (strain 12067; GenBank accession no.…”

Section: Methodsmentioning

confidence: 99%

Listeria monocytogenes Serotype Identification by PCR

2003

View full text Add to dashboard Cite

Serotyping is a universally accepted subtyping method for Listeria monocytogenes. Identification of the strain serotype permits differentiation between important food-borne strains (1/2a, 1/2b, and 4b) and provides a "gold standard" for comparing isolates analyzed in different labs and with different techniques. Although an efficient enzyme-linked immunosorbent assay serotyping protocol was described recently, identification of PCR serotyping primers would further increase the ease and accessibility of this classification system. Serotyping PCR primers were designed from variable regions of the L. monocytogenes genome. Three primer sets were used in conjunction with a previously described Division III primer set in order to classify 122 L. monocytogenes strains into five serotype groups [1/2a(3a), 1/2b, 1/2c(3c), 4b(d,e), and 4a/c]. Results of the PCR method agreed with those of the conventional slide agglutination method for 97, 100, 94, and 91% of strains belonging to serotypes 1/2a, 1/2b, 1/2c, and 4b, respectively.

show abstract

Section: Methodsmentioning

confidence: 99%

Listeria monocytogenes Serotype Identification by PCR

2003

View full text Add to dashboard Cite

show abstract

“…The elucidation of flagellin glycosylation in L. monocytogenes resulted from earlier reports on the discrepancy in molecular weight of its flagellin FlaA, which suggested a posttranslational modification (377,378). In 2004, the flagellins of listerial serotypes 1/2a, 1/2b, 1/2c, and 4b were shown to be ␤-O-glycosylated.…”

Section: Protein Glycosylationmentioning

confidence: 99%

The Sweet Tooth of Bacteria: Common Themes in Bacterial Glycoconjugates

Tytgat

Lebeer

2014

Microbiol Mol Biol Rev

129

View full text Add to dashboard Cite

SUMMARY Humans have been increasingly recognized as being superorganisms, living in close contact with a microbiota on all their mucosal surfaces. However, most studies on the human microbiota have focused on gaining comprehensive insights into the composition of the microbiota under different health conditions (e.g., enterotypes), while there is also a need for detailed knowledge of the different molecules that mediate interactions with the host. Glycoconjugates are an interesting class of molecules for detailed studies, as they form a strain-specific barcode on the surface of bacteria, mediating specific interactions with the host. Strikingly, most glycoconjugates are synthesized by similar biosynthesis mechanisms. Bacteria can produce their major glycoconjugates by using a sequential or an en bloc mechanism, with both mechanistic options coexisting in many species for different macromolecules. In this review, these common themes are conceptualized and illustrated for all major classes of known bacterial glycoconjugates, with a special focus on the rather recently emergent field of glycosylated proteins. We describe the biosynthesis and importance of glycoconjugates in both pathogenic and beneficial bacteria and in both Gram-positive and -negative organisms. The focus lies on microorganisms important for human physiology. In addition, the potential for a better knowledge of bacterial glycoconjugates in the emerging field of glycoengineering and other perspectives is discussed.

show abstract

“…This Gram-positive bacterium responsible for listerioisis can survive in numerous adverse environments including high salts, and pH and temperature extremes. The organism produces four to six simple peritrichous flagella when grown at <30 u C. Initial observations on SDS-PAGE of the flagellin protein indicated discrepancy in mass from that predicted (Dons et al, 1992;Peel et al, 1988) and prompted the structural characterization of purified flagellin protein (Schirm et al, 2004b). In distinct contrast to the previous examples, where either novel monosaccharides or complex glycans were shown to be attached to the flagellin protein, the flagellin of L. monocytogenes is glycosylated at up to six sites per monomer, which are localized to the central region of the protein, with b-O-linked GlcNAc.…”

Section: Gram-positive Bacteriamentioning

confidence: 99%

Flagellar glycosylation – a new component of the motility repertoire?

2006

View full text Add to dashboard Cite

The biosynthesis, assembly and regulation of the flagellar apparatus has been the subject of extensive studies over many decades, with considerable attention devoted to the peritrichous flagella of Escherichia coli and Salmonella enterica. The characterization of flagellar systems from many other bacterial species has revealed subtle yet distinct differences in composition, regulation and mode of assembly of this important subcellular structure. Glycosylation of the major structural protein, the flagellin, has been shown most recently to be an important component of numerous flagellar systems in both Archaea and Bacteria, playing either an integral role in assembly or for a number of bacterial pathogens a role in virulence. This review focuses on the structural diversity in flagellar glycosylation systems and demonstrates that as a consequence of the unique assembly processes, the type of glycosidic linkage found on archaeal and bacterial flagellins is distinctive. IntroductionThe bacterial flagellum, a key component of bacterial motility, is recognized as playing a vital role in the exquisite ability of many bacteria to adapt to the great diversity of biological niches in which they are found. This diversity includes aquatic and soil environments as well as the unique microenvironments which bacterial pathogens colonize within their respective protozoal, plant or animal host(s). While the model organisms for understanding the process of flagellar biosynthesis, assembly and regulation have been Salmonella enterica serovar Typhimurium strain LT2 and Escherichia coli K-12 (Aldridge & Hughes, 2002;Macnab, 2003), both of which produce peritrichous flagella composed of a single flagellin structural protein (simple flagella), recent post-genomic analysis has indicated that this existing paradigm reflects in general terms a degree of structural and regulatory conservation across the bacterial phyla (Pallen et al., 2005). However, the definitive contribution of the flagellar organelle to numerous unique bacterial lifestyles remains to be established. It is possible that the wellestablished paradigm defined by the motility systems of these two organisms may not be adequately encompassing for flagellar/motility systems from many distantly related bacterial species or indeed for alternative structures (lateral, polar or periplasmic flagella) of more closely related organisms. A recent body of work has described the process of flagellar glycosylation in a diverse number of bacterial species and in some cases has demonstrated a role for this process in both flagellar assembly and biological function. While it may be premature to consider glycosylation as a component of the flagellar repertoire, the intent of this review is to provide a current update on the extent of flagellar glycosylation in both Bacteria and Archaea, and to demonstrate that for a number of motile organisms it does appear to play a significant role both in flagellar assembly and in interactions of organisms with their surroundings. Flagellar structuresA va...

show abstract

Cloning and characterization of a gene encoding flagellin of Listeria monocytogenes

Cited by 81 publications

References 47 publications

Listeria monocytogenes Serotype Identification by PCR

Listeria monocytogenes Serotype Identification by PCR

The Sweet Tooth of Bacteria: Common Themes in Bacterial Glycoconjugates

Flagellar glycosylation – a new component of the motility repertoire?

Contact Info

Product

Resources

About