Cloning and characterization of an abundant Plasmodium knowlesi antigen which cross reacts with Gambian sera

Sequence polymorphism has been reported for virtually all malaria antigens and, in the case of the circumsporozoite (CS) protein, this variation is in the form of point mutations concentrated primarily in several regions recognized by T cells. The factors responsible for the variation are unknown. We studied the T cell responses to all known variants in malaria-exposed Thais. Memory CD4+ T cells responded to variants of a polymorphic immunodominant region (denoted Th2R), and CD4+ T cell clones specific for one Thai Th2R variant were generated. There was minimal cross-reactivity to any of the naturally occurring variants, including the other Thai variant, and competition studies performed with the clones using analog peptides demonstrated that all the substitutions of the polymorphic residues modulate either the binding of the peptide to major histocompatibility complex (MHC) molecules or the recognition by the T cell receptor of the peptide-MHC complex. Our data suggest that CD4+ T cells may be able to select parasites expressing variant sequences and have implications for development of a CS-based vaccine.

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Natural amino acid polymorphisms of the circumsporozoite protein of Plasmodium falciparum abrogate specific human CD4⁺ T cell responsiveness

Zevering

Khamboonruang

Good

1994

Eur J Immunol

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Plasmodium vivax, P. cynomolgi, andP. knowlesi:Identification of Homologue Proteins Associated with the Surface of Merozoites

Barnwell

Galinski

De-Simone

et al. 1999

Experimental Parasitology

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Merozoite surface protein-9 of Plasmodium vivax and related simian malaria parasites is orthologous to p101/ABRA of P. falciparum☆

Vargas-Serrato

Barnwell

Ingravallo³

et al. 2002

Molecular and Biochemical Parasitology

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Cloning and characterization of an abundant Plasmodium knowlesi antigen which cross reacts with Gambian sera

Cited by 3 publications

References 26 publications

Natural amino acid polymorphisms of the circumsporozoite protein of Plasmodium falciparum abrogate specific human CD4⁺ T cell responsiveness

Natural amino acid polymorphisms of the circumsporozoite protein of Plasmodium falciparum abrogate specific human CD4⁺ T cell responsiveness

Plasmodium vivax, P. cynomolgi, andP. knowlesi:Identification of Homologue Proteins Associated with the Surface of Merozoites

Merozoite surface protein-9 of Plasmodium vivax and related simian malaria parasites is orthologous to p101/ABRA of P. falciparum☆

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Cloning and characterization of an abundant Plasmodium knowlesi antigen which cross reacts with Gambian sera

Cited by 3 publications

References 26 publications

Natural amino acid polymorphisms of the circumsporozoite protein of Plasmodium falciparum abrogate specific human CD4+ T cell responsiveness

Natural amino acid polymorphisms of the circumsporozoite protein of Plasmodium falciparum abrogate specific human CD4+ T cell responsiveness

Plasmodium vivax, P. cynomolgi, andP. knowlesi:Identification of Homologue Proteins Associated with the Surface of Merozoites

Merozoite surface protein-9 of Plasmodium vivax and related simian malaria parasites is orthologous to p101/ABRA of P. falciparum☆

Contact Info

Product

Resources

About

Natural amino acid polymorphisms of the circumsporozoite protein of Plasmodium falciparum abrogate specific human CD4⁺ T cell responsiveness

Natural amino acid polymorphisms of the circumsporozoite protein of Plasmodium falciparum abrogate specific human CD4⁺ T cell responsiveness