Plasmodium vivax, P. cynomolgi, andP. knowlesi:Identification of Homologue Proteins Associated with the Surface of Merozoites

Barnwell, John W.; Galinski, Mary R.; De-Simone, Salvatore Giovanni; Perler, Francine B.; Ingravallo, Paul

doi:10.1006/expr.1998.4372

Cited by 53 publications

(46 citation statements)

References 52 publications

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“…Association between antibody responses to PvMSP-9 and P. vivax PvMSP-9 is associated with the surface of the merozoite and contains a hydrophobic signal sequence, a highly conserved N-terminal domain with a cluster of four cysteines, and a C-terminal region containing two speciesspecific blocks of repeats, designated PvMSP-9 RI and PvMSP-9 RII [57,58]. Recombinant proteins may represent individual blocks or both blocks (PvMSP-9 RIRII ).…”

Section: Cohort Studiesmentioning

confidence: 99%

Immunological markers of Plasmodium vivax exposure and immunity: a systematic review and meta-analysis

Cutts¹,

Powell²,

Agius³

et al. 2014

BMC Medicine

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Background: Identifying Plasmodium vivax antigen-specific antibodies associated with P. vivax infection and protective immunity is key to the development of serosurveillance tools and vaccines for malaria. Antibody targets of P. vivax can be identified by seroepidemiological studies of individuals living in P. vivax-endemic areas, and is an important strategy given the limited ability to culture P. vivax in vitro. There have been numerous studies investigating the association between P. vivax antibody responses and P. vivax infection, but there has been no standardization of results to enable comparisons across populations. Methods: We performed a systematic review with meta-analysis of population-based, cross-sectional, case-control, and cohort studies of individuals living in P. vivax-endemic areas. We searched 6 databases and identified 18 studies that met predefined inclusion and quality criteria, and examined the association between antibody responses to P. vivax antigens and P. vivax malaria.

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Section: Cohort Studiesmentioning

confidence: 99%

Immunological markers of Plasmodium vivax exposure and immunity: a systematic review and meta-analysis

Cutts¹,

Powell²,

Agius³

et al. 2014

BMC Medicine

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“…The completion of the Plasmodium falciparum genome has provided the basis for the identification of many proteins in sporozoites (5, 12), but very few of these proteins are characterized for their function in sporozoite infectivity and development in the liver. Proteomic and transcript analyses have identified a number of apical organelle and membraneassociated proteins expressed both in sporozoites and merozoites, with many belonging to molecular families conserved among the diverse species of Plasmodium (3,5,7,19,(22)(23)(24).The circumsporozoite protein (CSP) and to a lesser extent the thrombospondin-related anonymous protein (TRAP, or sporozoite surface protein 2 [SSP2]) have been the focus of most research on the sporozoite stages. CSP and TRAP are major sporozoite proteins that are functionally important for sporozoite development in the mosquito stage and are widely considered as important targets for vaccine development.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Antibodies against MAEBL Ligand Domains M1 and M2 Inhibit Sporozoite Development In Vitro

Preiser¹,

Rénia

Singh

et al. 2004

Infect Immun

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MAEBL is a type 1 membrane protein that is implicated in the merozoite invasion of erythrocytes and sporozoite invasion of mosquito salivary glands. This apical organelle protein is structurally similar to the ebl erythrocyte binding proteins, such as EBA-175, except that the tandem ligand domains of MAEBL are similar to part of the extracellular domain of apical membrane antigen 1 and not the Duffy binding-like domain. Although midgut and salivary gland sporozoites are morphologically similar, salivary gland sporozoites undergo a period of new gene expression after infecting the salivary glands, display distinct phenotypic differences, and are more infectious for the mammalian host. The objectives of this project were to determine the molecular form of MAEBL in the infectious salivary gland sporozoites and whether the ligand has a role in the sporozoite development to exoerythrocytic stages in hepatocytes. We determined that MAEBL is newly expressed in salivary gland sporozoites and in a form distinct from what is present in the midgut sporozoites or present in erythrocytic stages. Both ligand domains (M1 and M2) were expressed as part of a full-length membrane form of MAEBL in the salivary gland sporozoites in contrast to the other stages that retain only the M2 ligand domain as part of the membrane form of the protein. Antisera developed against the cysteine-rich regions of the extracellular portion of MAEBL inhibited sporozoite development to exoerythrocytic forms in vitro. Together these data indicate that MAEBL has a role in this third developmental stage in the life cycle of the malaria parasite. Thus, MAEBL is another target for pre-erythrocytic-stage vaccine development against malaria parasites.Malaria is one of the most serious human diseases, causing several million deaths and clinical illness in hundreds of millions of people every year. Infection is spread from person to person by the bite of a Plasmodium-infected anopheline mosquito. Sporozoites injected by the mosquito must infect and develop in a hepatocyte, which can then initiate the bloodstage infection that causes the severe pathogenesis of malaria. Molecules in the apical organelles and on the surface of malaria sporozoites play a role in sporozoite motility and target this infective stage to the liver. The completion of the Plasmodium falciparum genome has provided the basis for the identification of many proteins in sporozoites (5, 12), but very few of these proteins are characterized for their function in sporozoite infectivity and development in the liver. Proteomic and transcript analyses have identified a number of apical organelle and membraneassociated proteins expressed both in sporozoites and merozoites, with many belonging to molecular families conserved among the diverse species of Plasmodium (3,5,7,19,(22)(23)(24).The circumsporozoite protein (CSP) and to a lesser extent the thrombospondin-related anonymous protein (TRAP, or sporozoite surface protein 2 [SSP2]) have been the focus of most research on the sporozoite stages. CSP ...

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“…Both parasites have 48-h asexual erythrocytic cycles in their respective hosts [16] and produce relapses from persistent liver stages (hypnozoites) [17]. Phylogenetic trees based on smallsubunit rRNA group the 2 parasites closely together [18], primers based on P. vivax sequences readily amplify homologous sequences from P. cynomolgi (J. Alger, personal communication), and proteins from P. cynomolgi are fundamentally similar in peptide sequence and function to those from P. vivax (e.g., merozoite surface protein 1) [18,19]. For these reasons, P. cynomolgi infection of the rhesus monkey has been used as a model of human P. vivax infection to study the biology of the hypnozoite stage responsible for relapse [17], to test molecular markers for relapse, and to test for drug efficacy [20].…”

Section: Transcriptome Profiles Of Host Gene Expression In a Monkey Mmentioning

confidence: 99%

Transcriptome Profiles of Host Gene Expression in a Monkey Model of Human Malaria

et al. 2005

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We used human microarrays to examine gene expression in a rhesus monkey model of human Plasmodium vivax malaria (P. cynomolgi in Macaca mulatta). Whole-blood cells were collected for extraction of RNA before infection, during both the initial liver phase of infection and bloodstream infection, and during the course of 2 bloodstream relapses. Clustering analysis showed that similarities in gene expression were greater at similar stages of the protocol for the 2 different monkeys than for the same monkey at different stages of the protocol. Interestingly, a large number of genes involved in RNA processing showed distinct down-regulation during the initial liver phase of infection. When only up-regulated genes were examined, there was evidence of an increasing number of "defense response" genes as the infection evolved but not of "cytoskeleton" genes (P

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Plasmodium vivax, P. cynomolgi, andP. knowlesi:Identification of Homologue Proteins Associated with the Surface of Merozoites

Cited by 53 publications

References 52 publications

Immunological markers of Plasmodium vivax exposure and immunity: a systematic review and meta-analysis

Immunological markers of Plasmodium vivax exposure and immunity: a systematic review and meta-analysis

Antibodies against MAEBL Ligand Domains M1 and M2 Inhibit Sporozoite Development In Vitro

Transcriptome Profiles of Host Gene Expression in a Monkey Model of Human Malaria

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