2004
DOI: 10.1074/jbc.m400128200
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Cloning and Characterization of Cytokeratins 8 and 19 in Adult Rat Striated Muscle

Abstract: We used degenerate primers for the amino-and carboxyl-terminal ends of the rod domains of intermediate filament proteins in reverse transcriptase-PCR experiments to identify and clone cytokeratins 8 and 19 (K8 and K19) from cardiac muscle of the adult rat. Northern blots showed that K8 has a 2.2-kb transcript and K19 has a 1.9-kb transcript in both adult cardiac and skeletal muscles. Immunolocalization of the cytokeratins in adult cardiac muscle with isoform-specific antibodies for K8 and K19 showed labeling a… Show more

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Cited by 53 publications
(68 citation statements)
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“…16, September 2005 4283 now prevalent cytokeratin filaments, recognized with antibodies to the myc epitope tag associated with K8 ( Figure 1, G-I), or with antibodies to hK19 (our unpublished data). This result, too, was independent of the DS-Red fusion partner, because DS-Red alone did not associate with the cytokeratins (Figure 1, J-L), and similar results have been obtained with a green fluorescent protein (GFP) tag (Ursitti et al, 2004). Thus, the Dys-ABD associated preferentially with cytokeratin filaments composed of K8 and K19 when these are present at high levels in COS-7 cells.…”
supporting
confidence: 65%
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“…16, September 2005 4283 now prevalent cytokeratin filaments, recognized with antibodies to the myc epitope tag associated with K8 ( Figure 1, G-I), or with antibodies to hK19 (our unpublished data). This result, too, was independent of the DS-Red fusion partner, because DS-Red alone did not associate with the cytokeratins (Figure 1, J-L), and similar results have been obtained with a green fluorescent protein (GFP) tag (Ursitti et al, 2004). Thus, the Dys-ABD associated preferentially with cytokeratin filaments composed of K8 and K19 when these are present at high levels in COS-7 cells.…”
supporting
confidence: 65%
“…Our experiments were designed to learn whether the ABD of dystrophin, Dys-ABD, interacts with cytokeratins, specifically with K8 and K19, which are expressed in striated muscles and concentrated at costameres (Ursitti et al, 2004). We used transfection of COS-7 cells in vitro and skeletal muscle fibers in vivo to study the association of the Dys-ABD with structures containing K8 and K19.…”
Section: Resultsmentioning
confidence: 99%
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