Cloning and Characterization of Polyphenoloxidase DNA from Agaricus brasiliensis S. Wasser et al. (Agaricomycetideae)

Matsumoto-Akanuma, Akiko; Yamagishi, Akihiko; Motoi, Masuro; Ohno, Naohito

doi:10.1615/intjmedmushr.v8.i1.90

Cited by 5 publications

(1 citation statement)

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“…Sequence-tagged site (STS) markers were developed on the basis of sequences available from the literature ( Foulongne-Oriol et al 2012c , 2014 ; Matsumoto-Akanuma et al 2006 ; Thongklang et al 2014a ). The cleaved amplified polymorphic sequence (CAPS) approach was used to reveal polymorphism in STS.…”

Section: Methodsmentioning

confidence: 99%

The Genetic Linkage Map of the Medicinal Mushroom Agaricus subrufescens Reveals Highly Conserved Macrosynteny with the Congeneric Species Agaricus bisporus

Foulongne-Oriol

Brito

Cabannes

et al. 2016

G3 Genes|Genomes|Genetics

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Comparative linkage mapping can rapidly facilitate the transfer of genetic information from model species to orphan species. This macrosynteny analysis approach has been extensively used in plant species, but few example are available in fungi, and even fewer in mushroom crop species. Among the latter, the Agaricus genus comprises the most cultivable or potentially cultivable species. Agaricus bisporus, the button mushroom, is the model for edible and cultivable mushrooms. We have developed the first genetic linkage map for the basidiomycete A. subrufescens, an emerging mushroom crop known for its therapeutic properties and potential medicinal applications. The map includes 202 markers distributed over 16 linkage groups (LG), and covers a total length of 1701 cM, with an average marker spacing of 8.2 cM. Using 96 homologous loci, we also demonstrated the high level of macrosynteny with the genome of A. bisporus. The 13 main LG of A. subrufescens were syntenic to the 13 A. bisporus chromosomes. A disrupted synteny was observed for the three remaining A. subrufescens LG. Electronic mapping of a collection of A. subrufescens expressed sequence tags on A. bisporus genome showed that the homologous loci were evenly spread, with the exception of a few local hot or cold spots of homology. Our results were discussed in the light of Agaricus species evolution process. The map provides a framework for future genetic or genomic studies of the medicinal mushroom A. subrufescens.

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