Cloning and characterization of repetitive DNA sequences from genomes of <i>Oryza minuta</i> and <i>Oryza australiensis</i>

Aswidinnoor, Hajrial; Nelson, Rebecca; Dallas, John F.; McIntyre, C. L.; Leung, Ho-fung; Gustafson, J.P.

doi:10.1139/g91-123

Cited by 17 publications

(3 citation statements)

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“…The presence of strong distinct signals in the hybridized lanes suggested that there are several families of DNA repeat sequences specific to the genome(s) of the two species complexes. The presence of genome-specific repeat sequences has been demonstrated for almost all the known genomes in rice (Zhao et al 1989;Aswidinnoor et al 1991). The relative abundance and distribution of such genome-specific repeat sequences are probably central to the observed high degree of divergence in these genomes.…”

Section: Divergence Of O Ridleyi and O Meyeriana Complex Speciesmentioning

confidence: 99%

Two new genomes in the Oryza complex identified on the basis of molecular divergence analysis using total genomic DNA hybridization

1997

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The genus Oryza to which cultivated rice belongs has 24 species (2n = 24 or 48), representing seven genomes (AA, BB, CC, EE, FF, BBCC and CCDD). The genomic constitution of five of these species is unknown. These five species have been grouped into two species complexes, the tetraploid ridleyi complex (O. ridleyi, O. longiglumis) and the diploid meyeriana complex (O. granulata, O. meyeriana, O. indandamanica). To evaluate the genomic structure of these species in terms of divergence at the molecular level vis-a-vis other known genomes of Oryza, we used the total genomic DNA hybridization approach. Total genomic DNA (after restriction digestion) of 79 accessions of 23 Oryza species, 6 related genera, 5 outgroup taxa (2 monocots, 3 dicots) and 6 F1s and BC1s derived from crosses of O. sativa with wild species were hybridized individually with 32P-labeled total genomic DNA from 12 Oryza species: O. ridleyi, O. longiglumis, O. granulata, O. meyeriana, O. brachyantha, O. punctata, O. officinalis, O. eichingeri, O. alta, O. latifolia, O. australiensis, and O. sativa. The labeled genomic DNAs representing the ridleyi and meyeriana complexes cross-hybridized best to all the accessions of their respective species, less to those representing other genomes of Oryza and related genera, and least to outgroup taxa. In general, the hybridization differential measured in terms of signal intensities was >50-fold under conditions that permit detection of 70-75% homologous sequences, both in the presence and in the absence of O. sativa DNA as competitor. In contrast, when total DNAs representing other Oryza genomes were used as probes, species of the O. ridleyi and O. meyeriana complexes did not show any significant cross-hybridization (<5%). These results demonstrate that the genome(s) of both of these complexes are highly diverged and distinct from all other known genomes of Oryza. We, therefore, propose new genomic designations for these two species complexes: GG for the diploid O. meyeriana complex and HHJJ for the allotetraploid O. ridleyi complex. The results also suggest that the uniqueness of these genomes is not restricted to species-specific highly repetitive DNA sequences, but also applies to dispersed sequences present in single or low to moderate copy numbers. Furthermore these appear to share relatively more genome-specific repeat sequences between themselves than with other genomes of rice. The study also demonstrates the potential of total genomic DNA hybridization as a simple but powerful tool, complementary to existing approaches, for ascertaining the genomic makeup of an organism.

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Section: Divergence Of O Ridleyi and O Meyeriana Complex Speciesmentioning

confidence: 99%

Two new genomes in the Oryza complex identified on the basis of molecular divergence analysis using total genomic DNA hybridization

1997

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“…Genomic-specific repetitive DNA sequences have also been cloned and characterized in other plants such as rice (Oryza spp.) (Aswidinnoor et al 1991). These sequences may be useful in monitoring alien gene introgression between species.…”

Section: Different Hybridizing Patterns In Various Speciesmentioning

confidence: 99%

Phylogeny of tall fescue and related species using RFLPs

Sleper

1994

Theoret. Appl. Genetics

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The wild species of tall fescue (Festuca arundinacea var.genuina Schreb.) represent a wide range of genetic variation and constitute potential germplasm for tall fescue improvement. Our objective was to evaluate genome specificity of the previously-identified DNA probes and to examine the phylogenetic relationship of tall fescue with six related species by using RFLP data. A total of 29 DNA probes from aPstI-genomic library of tall fescue were hybridized toEcoRI-orHindIII-digested DNA of 32 plants from sixFestuca species and fromLolium perenne L. Fifteen probes hybridized to all seven species. The remaining 14 probes showed differential hybridization patterns (i.e., ±), especially at the diploid and tetraploid levels. This hybridization pattern reflected genome divergence in these species. The DNA probes will be useful markers in breeding programs involving interspecific and intergeneric hybridization. Cluster analyses were performed using the average genetic distances calculated with the RFLP data from 53 probe-enzyme combinations. Generally, genotypes from the same species were grouped in the same cluster. These data indicated that tall fescue has a close relationship withF. pratensis Huds. (diploid),F. arundinacea var.glaucescens Boiss. (tetraploid), andL. perenne L. (diploid) and thatFestuca pratensis andL. perenne had the closest degree of relationship.

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“…Recently molecular markers have been utilized to study the genome differentiation in various plant species. Both single-copy (Song et al, 1988;Bonierbale et al, 1988) and repetitive DNA sequences (Aswidinnoor et al, 1991;Anamthawat-Jonsson andHeslop-Harrison, 1993 andMawal et al, 1995) have been used. Repetitive DNA is the major determinant of plant chromosome structure and of the nucleotype.…”

Section: Introductionmentioning

confidence: 99%

Use of Genome-specific Satellite DNA Sequences to Study the Nature and Extent of Genome Differentiation in Evolution of Lathyrus

Khan¹

1998

Pakistan J. of Biological Sciences

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A cloned restriction fragment of a highly repetitive satellite DNA sequence from L. tingitanus was used as a molecular probe to study the nature and extent of genome differentiation in 22 Lathyrus species and 17 natural population of L. sativus. Sequences homologous to the satellite DNA were identified in the genomes of all Lathyrus species and natural population of L. sativus. However, substantial base sequence divergence was found in sequences homologous to the satellite DNA in different species. This was evident in the large variation in restriction fragment lengths. Similarity indices calculated from the restriction fragment length distributions were compared with the known taxonomical relationship between species established from morphological and molecular studies. The degree of genome differentiation was significantly small between population when compared to the greater variation between species.

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Cloning and characterization of repetitive DNA sequences from genomes of Oryza minuta and Oryza australiensis

Cited by 17 publications

References 0 publications

Two new genomes in the Oryza complex identified on the basis of molecular divergence analysis using total genomic DNA hybridization

Two new genomes in the Oryza complex identified on the basis of molecular divergence analysis using total genomic DNA hybridization

Phylogeny of tall fescue and related species using RFLPs

Use of Genome-specific Satellite DNA Sequences to Study the Nature and Extent of Genome Differentiation in Evolution of Lathyrus

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