Cloning and Chromosomal Mapping of Three Novel Genes, GPR9, GPR10, and GPR14, Encoding Receptors Related to Interleukin 8, Neuropeptide Y, and Somatostatin Receptors

Marchese, Adriano; Heiber, Michael; Nguyen, Tuan; Heng, Henry H.Q.; Saldivia, Victor; Cheng, Regina; Murphy, Philip M.; Tsui, Lap‐Chee; Shi, Xiaomei; Gregor, Paul; George, Susan R.; O’Dowd, Brian F.; Docherty, John M.

doi:10.1006/geno.1995.9996

Cited by 176 publications

(110 citation statements)

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“…Two conflicting chromosomal localizations have been reported for human CXCR3, 8p12-p11.2 and Xq13. 30,31 Because of the low allele frequency of 875A, it was considered highly unlikely that this individual was homozygous for the 875A allele of CXCR3 located on 8p. In order to confirm that CXCR3 is located on X chromosome, the genotypes of his parents were examined.…”

Section: Assignment Of Human Cxcr3 Gene On Chromosome Xmentioning

confidence: 99%

“…CXCR3 was initially reported to localize at 8p12-p11.2 by fluorescence in situ hybridization (FISH) using the genomic DNA clone GPR9 as the hybridization probe. 30 However, it was later reported to be located on chromosome X by PCR analysis using hamster/human hybrid lines, 34 and subsequently localized at Xq13 by FISH. 31 Our data of SSCP analysis strongly supported the latter, although the possibility that the father (shown in Figure 1e, Lane 3) has a deletion in one autosome (null allele), and that the null allele was transmitted to the proband, cannot be completely excluded.…”

Section: Cxcr1-827g/c (276s/t) and Ccr3-827g-828c/827c-828g (276s/t);mentioning

confidence: 99%

“…The specific primer pairs were designed according to the published genomic DNA sequences: CXCR1 (GenBank accession number: L19592), 24 CXCR2 (M99412), 28 GPR9 (U32674). 30 Both CXCR1 and CXCR2 have no intron within their coding regions of 1053 bp and 1083 bp, respectively. GPR9 corresponds to the putative CXCR3 exon 2 and consists of partial CXCR3 coding region [CXCR3 (5-368)] of 1095 bp and the 3′ untranslated region (3′UTR).…”

Section: Pcr-sscp Analysismentioning

confidence: 99%

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Single nucleotide polymorphisms in the coding regions of human CXC-chemokine receptors CXCR1, CXCR2 and CXCR3

2000

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Chemokines and their receptors have critical roles in inflammatory and immunological responses, and thus their genetic contribution to various human disorders needs investigation. In this study, systematic variation screening of the entire coding regions of CXCR1 (IL8RA), CXCR2 (IL8RB) and CXCR3 was carried out, using genomic DNA from a large number of Japanese healthy individuals and patients with rheumatic diseases. In addition to the previously reported variations in CXCR1 and in CXCR2, two non-synonymous, two synonymous substitutions and one nonsense mutation of CXCR1, one non-synonymous and two synonymous substitutions of CXCR2, two non-synonymous substitutions of CXCR3 were newly identified. The common single nucleotide polymorphisms (SNPs) at CXCR1 codon 827 and CXCR2 codon 786 were in strong linkage disequilibrium. In addition, familial analysis indicated that human CXCR3 is located on chromosome X. No significant association was observed between the variations and the tested rheumatic diseases. However, CXCR variations identified in this study will provide valuable information for the future studies in medical sciences as well as in human genetics. Genes and Immunity (2000) 1, 330-337.

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Section: Assignment Of Human Cxcr3 Gene On Chromosome Xmentioning

confidence: 99%

Section: Cxcr1-827g/c (276s/t) and Ccr3-827g-828c/827c-828g (276s/t);mentioning

confidence: 99%

Section: Pcr-sscp Analysismentioning

confidence: 99%

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Single nucleotide polymorphisms in the coding regions of human CXC-chemokine receptors CXCR1, CXCR2 and CXCR3

2000

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“…The characterization of GPR14, a novel GPCR homologous to a rat`orphan' receptor originally designated SENR or GPR14 (Tal et al, 1995;Marchese et al, 1995), as a selective U-II receptor was originally made by Ames et al (1999) and con®rmed subsequently by several other groups using rat GPR14 Mori et al, 1999;Nothacker et al, 1999). As with U-II, GPR14 expression is evident within the cardiovascular system.…”

Section: Introductionmentioning

confidence: 99%

“…Further, in addition to any putative paracrine action, U-II likely functions as an endocrine factor since U-II-like immunoreactivity has been detected within ®sh plasma (*30 pM; Kobayashi et al, 1986;Winter et al, 1999). Irrespectively, however, the fact that the cognate receptor for U-II, GPR14, is expressed within the cardiovasculature (Ames et al, 1999) implies that the heart and peripheral vasculature represent`target organs' for this hormone.The characterization of GPR14, a novel GPCR homologous to a rat`orphan' receptor originally designated SENR or GPR14 (Tal et al, 1995;Marchese et al, 1995), as a selective U-II receptor was originally made by Ames et al (1999) and con®rmed subsequently by several other groups using rat GPR14 Mori et al, 1999;Nothacker et al, 1999). As with U-II, GPR14 expression is evident within the cardiovascular system.…”

mentioning

confidence: 99%

Differential vasoconstrictor activity of human urotensin‐II in vascular tissue isolated from the rat, mouse, dog, pig, marmoset and cynomolgus monkey

Douglas

Sulpizio

Piercy

et al. 2000

British J Pharmacology

212

176

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1 Urotensin-II (U-II) and its G-protein-coupled receptor, GPR14, are expressed within mammalian cardiac and peripheral vascular tissue and, as such, may regulate mammalian cardiovascular function. The present study details the vasoconstrictor pro®le of this cyclic undecapeptide in di erent vascular tissues isolated from a diverse range of mammalian species (rats, mice, dogs, pigs, marmosets and cynomolgus monkeys). 2 The vasoconstrictor activity of human U-II was dependent upon the anatomical origin of the vessel studied and the species from which it was isolated. In the rat, constrictor responses were most pronounced in thoracic aortae and carotid arteries: 7log[EC 50 ]s 9.09+0.19 and 8.84+0.21, R max s 143+21 and 67+26% 60 mM KCl, respectively (compared, for example, to 7log[EC 50 ] 7.90+0.11 and R max 142+12% 60 mM KCl for endothelin-1 [ET-1] in thoracic aortae). Responses were, however, absent in mice aortae (7log [EC 50 ] 56.50). These ®ndings were further contrasted by the observation that U-II was a`coronary-selective' spasmogen in the dog (7log [EC 50 ] 9.46+0.11, R max 109+23% 60 mM KCl in LCX coronary artery), yet exhibited a broad spectrum of vasoconstrictor activity in arterial tissue from Old World monkeys (7log [EC 50 ]s range from 8.96+0.15 to 9.92+0.13, R max s from 43+16 to 527+135% 60 mM KCl). Interestingly, signi®cant di erences in reproducibility and vasoconstrictor e cacy were seen in tissue from pigs and New World primates (vessels which responded to noradrenaline, phenylephrine, KCl or ET-1 consistently). 3 Thus, human U-II is a potent, e cacious vasoconstrictor of a variety of mammalian vascular tissues. Although signi®cant species/anatomical variations exist, the data support the hypothesis that U-II in¯uences the physiological regulation of mammalian cardiovascular function. British Journal of Pharmacology (2000) 131, 1262 ± 1274 Keywords: Urotensin-II; GPR14; SENR; endothelin-1; somatostatin; vascular reactivity; spasmogen; coronary artery; endothelium; vasoconstriction Abbreviations: FLIPR,¯uorescent imaging plate reader; GPCR, guanosine triphosphate-binding protein [G-protein]-coupled receptor; LAD coronary artery, left anterior descending coronary artery; LCX, left circum¯ex coronary artery; SENR, sensory epithelial neuropeptide-like receptor; U-II, Urotensin-II IntroductionThe integrated control of cardiovascular homeostasis is achieved through a combination of direct neuronal control and systemic activation of the neurohumoral axis. The principal mammalian vasoactive factors of this axis (angiotensin-II, endothelin [ET]-1, noradrenaline) exert their haemodynamic e ects exclusively via interactions with speci®c seven transmembrane heterotrimeric G-protein-coupled receptors (GPCRs). Drugs which antagonize such interactions constitute one of the most successful classes of therapeutic agents identi®ed to date (Stadel et al., 1997; Wilson et al., 1998). Nowhere is this more evident than within the vasculature where numerous agents have been developed successfully for the clinical ...

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Lymphocyte-specific chemokine receptor CXCR3: regulation, chemokine binding and gene localization

et al. 1998

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Expression of CXCR3, the receptor for the CXC chemokines IFN-gamma-inducible 10-kDa protein (IP10) and monokine induced by IFN-gamma (Mig), in human T lymphocytes and their responses to IP10 and Mig were analyzed. About 40 % of resting T lymphocytes (and low numbers of B cells and natural killer cells) stained positive for CXCR3 but these cells did not express CXCR3 transcripts and did not respond to these chemokines. However, treatment with IL-2 with or without addition of phytohemagglutinin for 10 or more days resulted in cultures of fully responsive, CXCR3-positive T lymphocytes. Treatment with anti-CD3 antibodies in the presence or absence of soluble anti-CD28 antibodies was inhibitory. Addition of chondroitin sulfate C to CXCR3-expressing murine pre-B cells allowed the determination of high-affinity binding for Mig and IP10 with Kd of 0.9-1.2 nM and 0.2-0.3 nM, respectively, and 1.3 x 10(4) binding sites per cell. The gene for CXCR3 was localized on human chromosome Xq13 which is in clear contrast to all other chemokine receptor genes, suggesting unique function(s) for this receptor and its ligands that may lie beyond their established role in T cell-dependent immunity.

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Cloning and Chromosomal Mapping of Three Novel Genes, GPR9, GPR10, and GPR14, Encoding Receptors Related to Interleukin 8, Neuropeptide Y, and Somatostatin Receptors

Cited by 176 publications

References 0 publications

Single nucleotide polymorphisms in the coding regions of human CXC-chemokine receptors CXCR1, CXCR2 and CXCR3

Single nucleotide polymorphisms in the coding regions of human CXC-chemokine receptors CXCR1, CXCR2 and CXCR3

Differential vasoconstrictor activity of human urotensin‐II in vascular tissue isolated from the rat, mouse, dog, pig, marmoset and cynomolgus monkey

Lymphocyte-specific chemokine receptor CXCR3: regulation, chemokine binding and gene localization

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