Cloning and expression analysis of the mouse T-box gene Tbx18

Abstract

T-box genes encode transcription factors that regulate a variety of developmental processes. In this report, we describe the cloning and expression analysis of the novel mouse T-box gene Tbx18. During development expression is most prominent in the proepicardial organ and in the epicardium of the heart. Other sites of expression include the cranial paraxial mesoderm, the presomitic mesoderm, the anterior somite half, the genital ridge, and the developing limb buds.

Ror2 knockout mouse as a model for the developmental pathology of autosomal recessive Robinow syndrome

“…Tbx18 expression in the anterior part of the somites (Kraus et al, 2001) was reduced in the mutant (Fig. 5I,J).…”

“…Probes were as follows: rat Col2 (Kohno et al, 1984), Col10 (Jacenko et al, 1993), Ihh (Bitgood and McMahon, 1995), Fgf4 (Niswander et al, 1994), Gli1 (Hui et al, 1994), Mesp2 (Saga et al, 1997), Myf5 (Montarras et al, 1991), Notch1 (Conlon et al, 1995), Paraxis (Burgess et al, 1995), Uncx4.1 (Mansouri et al, 1997), and Tbx18 (Kraus et al, 2001).…”

Isolation, Characterization and Differentiation Potential of Cardiac Progenitor Cells in Adult Pigs

“…Since these T-box genes are expressed in the epicardial cell layer of the heart and its progenitors (Plageman et al 2005;Kraus et al 2001;Hatcher et al 2004;Tanaka et al 2004), PACPs may belong to an epicardial progenitor population ( Figure 28, Table 7). …”

“…Together, these T-box genes likely contribute to cardiac lineage determination, chamber specification, valve formation, epicardial development and specialization of the conduction system (Plageman et al 2005). T-box genes, including Tbx18 and Tbx5 are also expressed in the epicardial cell layer of the heart and its progenitors (Kraus et al 2001;Hatcher et al 2004;Tanaka and Tickle 2004 Figure 4. Illustration of stem cell control by the niche (Secker et al 2009).…”

The cleft lip and palate defects inDancermutant mice result from gain of function of theTbx10gene

“…This finding suggests that the downstream effects of transcriptional modulation of any target gene may be highly specific to the developing craniofacial region. Alternatively, ectopic Tbx10 expression may antagonize the function of other closely related T-box factors, such as Tbx1, Tbx15, Tbx18, and Tbx22, which all share high amino acid sequence identity in their DNA-binding domains and are all expressed in partially overlapping patterns during craniofacial development (39)(40)(41)(42)(43). Mutations in Tbx1 and TBX22 cause craniofacial defects that include cleft palate in mice and humans, respectively (34,35).…”