Isolation, Characterization and Differentiation Potential of Cardiac Progenitor Cells in Adult Pigs

Heart Regeneration with Embryonic Cardiac Progenitor Cells and Cardiac Tissue Engineering

“…Nevertheless, adult CPC is considered as an ideal cell source for cell therapy (Figure 2). Adult CPCs have been successfully isolated from cardiac biopsies of mice [64], rat [65], sheep [66], pig [67] and human [68]. Despite small numbers of adult CPCs that can be derived from the biopsies, they can be propagated for long periods of time to obtain sufficient cells for transplantation [65,69].…”

The Potential of Stem Cells in the Treatment of Cardiovascular Diseases

“…Cardiac Mesoangioblasts are vessel-associated mesenchymal stem cells that derived from embryonic dorsal aorta [ 47 , 87 ], postnatal aorta [ 88 ], heart [ 46 , 47 , 50 ], skeletal muscle [ 89 , 90 ] and are able to differentiate efficiently into a mature CMCs [ 47 ]. It can be passaged and expanded in vitro indefinitely.…”

“…[ 2 , 17 , 46 , 47 ]. So far, several research groups have reported the isolation of cardiac stem like cells from various species such as mice, rat, dog, pigs and human hearts based on the cell surface antigens, stem cell antigen 1 (Sca1 [ 2 , 46 – 50 ], Abcg2 [ 51 , 52 ], and ckit [ 6 , 7 , 17 ].…”

“…It has been described at least 7 different types of CSCs, including the pan-stem cell marker ckit + cells [ 17 , 53 , 54 ]; the cell surface marker stem cell antigen Sca1 + cells [ 2 , 48 ]; the transcription factor Isl1+ cells [ 5 , 55 ], the cardiac side population that possess physiological properties to efflux fluorescent dye (Abcg2+/Mdr1+) [ 52 , 56 , 57 ], the cardiac mesoangioblasts [ 47 , 50 ], the cardiosphere-derived stem cells (ckit+/Sca1+/Flk1+) [ 7 , 58 ], and the epicardial progenitors [ 59 , 60 ] have been isolated and characterized from hearts by different laboratories [ 54 , 61 – 63 ].…”

Caracterización y evaluación del potencial terapéutico de células madre mesenquimales obtenidas de tejido adiposo en el tratamiento del infarto agudo de miocardio en modelo porcino

“…Otro tipo de células asociadas con el corazón son los mesangioblastos, una clase de células madre asociadas a los vasos capaces de diferenciarse a varios tipos celulares mesodérmicos. Durante este mismo año, se ha estudiado el aislamiento, caracterización y capacidad de diferenciación en modelo porcino de distintas CSC (Vanelli A, 2012). También ha sido analizado el crecimiento de estas CSC, obtenidas de biopsias de corazones humanos, en condiciones fisiológicas (bajas concentraciones de O 2 ), obteniéndose resultados similares a los obtenidos con MSC humanas y de ratón .…”