Heart Regeneration with Embryonic Cardiac Progenitor Cells and Cardiac Tissue Engineering

Tian, Shuo; Liu, Qihai; Gnatovskiy, Leonid; Peter, X.; Wang, Zhong

doi:10.19104/jstb.2015.104

Cited by 17 publications

(15 citation statements)

References 141 publications

(139 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Persistence by cell fusion in neonatal injections is unlikely since cCICs often appear in large clusters (ranging from 100 to 500 μm), and numerous simultaneous cell fusion events all occurring at same location would be unprecedented. Cell death due to inflammation, apoptosis, or necrosis is a major cause for postinjection cell loss, but scant evidence of these processes in donated cCIC (Figure S5) is consistent with their prolonged persistence in the postnatal heart.…”

Section: Discussionmentioning

confidence: 85%

Adaptation within embryonic and neonatal heart environment reveals alternative fates for adult c-kit+ cardiac interstitial cells

Wang

Álvarez

Muliono

et al. 2019

Stem Cells Translational Medicine

View full text Add to dashboard Cite

Cardiac interstitial cells (CICs) perform essential roles in myocardial biology through preservation of homeostasis as well as response to injury or stress. Studies of murine CIC biology reveal remarkable plasticity in terms of transcriptional reprogramming and ploidy state with important implications for function. Despite over a decade of characterization and in vivo utilization of adult c-Kit + CIC (cCIC), adaptability and functional responses upon delivery to adult mammalian hearts remain poorly understood. Limitations of characterizing cCIC biology following in vitro expansion and adoptive transfer into the adult heart were circumvented by delivery of the donated cells into early cardiogenic environments of embryonic, fetal, and early postnatal developing hearts. These three developmental stages were permissive for retention and persistence, enabling phenotypic evaluation of in vitro expanded cCICs after delivery as well as tissue response following introduction to the host environment. Embryonic blastocyst environment prompted cCIC integration into trophectoderm as well as persistence in amniochorionic membrane. Delivery to fetal myocardium yielded cCIC perivascular localization with fibroblast-like phenotype, similar to cCICs introduced to postnatal P3 heart with persistent cell cycle activity for up to 4 weeks. Fibroblast-like phenotype of exogenously transferred cCICs in fetal and postnatal cardiogenic environments is consistent with inability to contribute directly toward cardiogenesis and lack of functional integration with host myocardium. In contrast, cCICs incorporation into extraembryonic membranes is consistent with fate of polyploid cells in blastocysts. These findings provide insight into cCIC biology, their inherent predisposition toward fibroblast fates in cardiogenic environments, and remarkable participation in extraembryonic tissue formation.

show abstract

Section: Discussionmentioning

confidence: 85%

Adaptation within embryonic and neonatal heart environment reveals alternative fates for adult c-kit+ cardiac interstitial cells

Wang

Álvarez

Muliono

et al. 2019

Stem Cells Translational Medicine

View full text Add to dashboard Cite

show abstract

“…In contrast, during injection into dense tissue, such as intramuscular injections, the host tissue may provide mechanical support and promote cell survival. However, injection into dense tissue requires higher injection pressures and can still result in cell leakage at the transplantation site [17]. Additionally, cells may be confronted with several other survival challenges during the acute post-injection stage that are not inherently mechanical in nature, including hypoxia, low nutrient transport, and the immune and inflammatory response [18-20].…”

Section: Mechanical Challenges To Successful Stem Cell Transplantationmentioning

confidence: 99%

The diverse roles of hydrogel mechanics in injectable stem cell transplantation

Foster

Marquardt

Heilshorn

2017

Current Opinion in Chemical Engineering

View full text Add to dashboard Cite

Stem cell delivery by local injection has tremendous potential as a regenerative therapy but has seen limited clinical success. Several mechanical challenges hinder therapeutic efficacy throughout all stages of cell transplantation, including mechanical forces during injection and loss of mechanical support post-injection. Recent studies have begun exploring the use of biomaterials, in particular hydrogels, to enhance stem cell transplantation by addressing the often-conflicting mechanical requirements associated with each stage of the transplantation process. This review explores recent biomaterial approaches to improve the therapeutic efficacy of stem cells delivered through local injection, with a focus on strategies that specifically address the mechanical challenges that result in cell death and/or limit therapeutic function throughout the stages of transplantation.

show abstract

“…Molecules (with repair and/or regeneration capacities of the damaged tissue) are encapsulated in the hydrogel and released locally over time (the hydrazone bond is then broken and occurs the molecules release from nanoparticles). In several studies it was reported that polymer matrices can be used to sustain the release of encapsulated molecules for up to 100 days and the release profi le initially showed a quick release followed by a slower release rate [6][7][8][9][10]. …”

Section: Introductionmentioning

confidence: 99%

Hydrogels for Cardiac Tissue Repair and Regeneration

Grigore¹

2017

J Cardiovasc Med Cardiol

View full text Add to dashboard Cite

The main cause of death in the world continues to be cardiovascular disease, which affects annually over 900,000 people and in the entire word approximately 50% of the people suffering myocardial infarction (MI) die within 5 years. MI causes a number of cardiac pathologies like hypertension, blocked coronary arteries and valvular heart diseases resulting ischemic cardiac injury. In the last years, cardiac tissue engineering has made considerable progress, because this progress have been made towards developing injectable hydrogels for the purpose of cardiac repair and/or regeneration. This study aims to provide an updated survey of the major progress in the fl ied of injectable cardiac tissue engineering, including biomaterials (natural, synthetic or hybrid hydrogels), their advantages or disadvantages and the main seeding cell sources. Also, this review focuses on the progress made in the fi eld of hydrogels for cardiac tissue repair and/or regeneration for MI over the last years.

show abstract

Heart Regeneration with Embryonic Cardiac Progenitor Cells and Cardiac Tissue Engineering

Cited by 17 publications

References 141 publications

Adaptation within embryonic and neonatal heart environment reveals alternative fates for adult c-kit+ cardiac interstitial cells

Adaptation within embryonic and neonatal heart environment reveals alternative fates for adult c-kit+ cardiac interstitial cells

The diverse roles of hydrogel mechanics in injectable stem cell transplantation

Hydrogels for Cardiac Tissue Repair and Regeneration

Contact Info

Product

Resources

About