Cloning and Expression Levels of Genes Relating to the Ovulation Rate of the Yunling Black Goat1

Cui, Huanxian; Zhao, Sumei; Cheng, Meng-Chun; Guo, Li; Ye, R.Q.; Liu, W.Q.; Shi-zheng, Gao

doi:10.1095/biolreprod.108.069021

Cited by 51 publications

(51 citation statements)

References 40 publications

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“…This symbolizes that non-synonymous sites evolved faster than the synonymous sites and positive selection effect over-shadows purifying selection. It is an implication that balancing selection (or positive Darwinian section) favoured new variants and increased allelic polymorphism [22] which in turn might induce a change in the conformation of protein structure, thereby affecting the signaling pathway during follicle differentiation and ovulation [23]. The 0.97 omega value of sheep observed in this study, signifies purifying selection i.e.…”

Section: Discussionmentioning

confidence: 64%

Computational Molecular Analysis of the Sequences of BMP15 Gene of Ruminants and Non-Ruminants

Bibinu¹,

Yakubu²,

Ugbo³

et al. 2016

OJGen

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Bone morphogenetic protein 15 (BMP15) is a member of the transforming growth factor β (TGFβ) super family that is expressed by oocytes and plays key roles in granulosa cell development and fertility in animal. This study investigated the molecular genetic variation of BMP15 gene of some selected mammalian species with a view to providing relevant genetic information for breeding and selection programmes in the studied species using computational methods. A total of thirty seven (37) BMP15 nucleotide sequences comprising goats (18), sheep (6), cattle (6), swine (4) and chicken (3) were retrieved from the GenBank. Sequence alignment, translation and comparison of the BMP15 gene of the various species were done with ClustalW. High degree of polymorphism of BMP15 gene was observed among the studied species. The significant value (P < 0.01) for relative proportions of non-synonymous substitutions per non-synonymous site (dN) and the number of synonymous substitutions per synonymous site (dS) symbolized that non-synonymous sites evolved faster than the synonymous sites and positive selection effect over shadowed purifying selection. Functional analysis of missense mutations using PROVEAN showed that twelve amino acid substitutions (L10S, W13A, E20L, V28S, P31R, P31G, P40Q, L42W, Q46N, A52V, R58C and G64T) in goats, nine in sheep (H21R, S32R, I33A, A39W, Q46W, E51A, G54S, R61D and E72A), six in cattle (Q30M, T41W, E50R, I62R, H65E, and E72S), seven in swine (I7L, T9I, V33I, L35H, C40P, R46P and Q61R) and five in chickens (A20H, L27H, W43L, A47P and G50Y) appeared beneficial. The phylogenetic trees from nucleotide and amino acid sequences revealed the close relatedness of members of the bovidae family (goat, sheep and cattle). The present information could guide future efforts involving * Corresponding authors. B. S. Bibinu et al. 40selection of markers of fecundity to improve genetically livestock species in Nigeria.

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Section: Discussionmentioning

confidence: 64%

Computational Molecular Analysis of the Sequences of BMP15 Gene of Ruminants and Non-Ruminants

Bibinu¹,

Yakubu²,

Ugbo³

et al. 2016

OJGen

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“…Consistent with the classic roles of FSH in steroidogenesis ( 19 ), Fshr is mainly expressed in the reproductive system (13)(14)(15)(16)(17). The receptor is also expressed in some nonreproductive organs and tissues ( 18,20 ) where it plays roles in regulating the development of the skeletal system and others (21)(22)(23)(24).…”

Section: Rt-pcrmentioning

confidence: 70%

FSH stimulates lipid biosynthesis in chicken adipose tissue by upregulating the expression of its receptor FSHR

Cui

Zhao

Liu

et al. 2012

Journal of Lipid Research

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“…Therefore, understanding the molecular mechanisms underlying such complex trait formation combined with functional gene discovery may provide valuable information for the breeder. FSH is a glycoprotein hormone mainly secreted by the pituitary gland to regulate gonadal development and function after delivery through the bloodstream (Meduri et al, 2002;Patsoula et al, 2003;Cui et al, 2009). Researchers have found that FSH can increase lipid accumulation in chicken abdominal fat by about 50% (Cui et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Follicle-stimulating hormone increases the intramuscular fat content and expression of lipid biosynthesis genes in chicken breast muscle

Cui

Liu

et al. 2016

J. Zhejiang Univ. Sci. B

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Intramuscular fat (IMF) is a crucial factor in the quality of chicken meat. The genetic basis underlying it is complex. Follicle-stimulating hormone (FSH), well-known as an effector in reproductive tissues, was recently discovered to stimulate abdominal fat accumulation in chicken. The effect of FSH on IMF accumulation and the underlying molecular regulatory mechanisms controlling both IMF and abdominal fat deposition in vivo are largely unknown. In this study, two groups of chickens were treated with chicken FSH or a placebo. The lipid content of breast muscle, abdominal fat volume, and serum concentrations of FSH were examined. Related genes implicated in breast muscle and abdominal fat accumulation were also investigated. Compared to the control group, the triglyceride (TG) content of breast muscle and the percentage of abdominal fat in FSH-treated chickens were significantly increased by 64.9% and 56.5% (P<0.01), respectively. The FSH content in the serum of FSH-treated chickens was 2.1 times than that of control chickens (P<0.01). Results from quantitative real-time polymerase chain reaction (qRT-PCR) assays showed that relative expression levels of fatty acid synthase (FAS), lipoprotein lipase (LPL), diacylglycerol acyltransferase 2 (DGAT2), adipocyte fatty acid binding protein (A-FABP), and peroxisome proliferator-activated receptor γ (PPARγ) were significantly upregulated in breast muscle following FSH treatment (P<0.01). Treatment with FSH also significantly increased relative expression levels of FAS, LPL, DGAT2, A-FABP, and PPARγ in abdominal fat tissue (P<0.05). The results of principal component analysis (PCA) for gene expression (breast muscle and abdominal fat) showed that the control and FSH treatment groups were well separated, which indicated the reliability of the data. This study demonstrates that FSH plays an important role in IMF accumulation in female chickens, which likely involves the regulation of biosynthesis genes related to lipid metabolism.

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Cloning and Expression Levels of Genes Relating to the Ovulation Rate of the Yunling Black Goat1

Cited by 51 publications

References 40 publications

Computational Molecular Analysis of the Sequences of BMP15 Gene of Ruminants and Non-Ruminants

Computational Molecular Analysis of the Sequences of BMP15 Gene of Ruminants and Non-Ruminants

FSH stimulates lipid biosynthesis in chicken adipose tissue by upregulating the expression of its receptor FSHR

Follicle-stimulating hormone increases the intramuscular fat content and expression of lipid biosynthesis genes in chicken breast muscle

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