Cloning and expression of a heat shock protein (HSP) 90 gene in the haemocytes of Crassostrea hongkongensis under osmotic stress and bacterial challenge

Fu, Di; Chen, Jinhui; Zhang, Yang; Zhou, Yu

doi:10.1016/j.fsi.2011.04.011

Cited by 88 publications

(38 citation statements)

References 34 publications

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“…In the present study, the expression level of PmHSP20 mRNA showed a wave-like pattern after challenge with the biological stressors, which might have resulted from two processes: an acute response related to the immune system and/or a chronic response related to body reparation. This wave-like pattern of HSP expression is similar to previous findings in other species such as HSP20 in clam (Venerupis philippinarum) (Li et al, 2010) and HSP90 in oyster (C. hongkongensis) (Fu et al, 2011). Although the exact transcriptional mechanisms of upregulation of PmHSP20 expression in hemocytes are still unknown, the sequential expression pattern of PmHSP20 might enhance its protective ability against environmental pathogens in P. martensii.…”

Section: Discussionsupporting

confidence: 87%

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Molecular cloning and expression analysis of heat shock protein 20 (HSP20) from the pearl oyster Pinctada martensii

Lei¹,

Wu²,

Liang³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Small heat shock proteins (HSPs) are molecular chaperones with ATP-independent properties. They are involved in a variety of physiological and stress processes. In this study, the full-length HSP 20 (HSP20) from Pinctada martensii, designated as PmHSP20, was obtained from hemocytes using rapid amplification of cDNA ends technology. The PmHSP20 cDNA was 952 bp in length, containing an open reading frame of 534 bp that encoded 177-amino acid residues, with an isoelectric point of 5.86 and molecular weight of 20.24 kDa. The sequence of this deduced polypeptide contained typical structure and function domains conserved in the HSP20 family, providing evidence that PmHSP20 belongs to the HSP20 family. The PmHSP20 mRNA expression levels were detected in various tissues of P. martensii and in hemocytes after challenges with the bacteria Vibrio harveyi and lipopolysaccharide (LPS) using quantitative realtime polymerase chain reaction amplification. The results indicated that PmHSP20 is constitutively expressed in all tissues tested and might be involved in the immune response. The upregulation of PmHSP20 after V. harveyi and LPS challenge suggests that PmHSP20 plays an important role in anti-bacterial immunity. Studies on PmHSP20 are a valuable resource to further explore the immune system in pearl oysters and might enhance our knowledge of molluscan innate immunity.

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Section: Discussionsupporting

confidence: 87%

“…Generally, the quantity of HSPs increases significantly after bacterial challenge such as has been observed for HSP90 in oyster (Crassostrea hongkongensis) (Fu et al, 2011) and for HSP70 in both bay scallop (Argopecten irradians) (Song et al, 2006) and pearl oyster Figure 6. Expression patterns of PmHSP20 mRNA after LPS stimulation.…”

Section: Discussionmentioning

confidence: 75%

Molecular cloning and expression analysis of heat shock protein 20 (HSP20) from the pearl oyster Pinctada martensii

Lei¹,

Wu²,

Liang³

et al. 2016

Genet. Mol. Res.

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“…The expression level of HSP90-2 in the 2 groups first increased and then progressively decreased, which was similar to that observed in the gill of C. hongkongesi (Fu et al, 2011) and the branchial lamellae of S. salar (Pan et al, 2000) under high salinity stress. For the WG (Figure 4A), there was a dramatic increase during the first 6 h (P < 0.05) after challenge, and reached a maximum at 6 h. Then, the expression level dropped to almost the initial level at 12 h, and even sharply decreased to the lowest level at 24 h (P < 0.05).…”

Section: Discussionsupporting

confidence: 80%

“…It is possible that the 4 ppt was so low that it fell beyond the salinity range that induces the production of HSP90-1; thus, disrupting protein synthesis in organisms (Huhtala et al, 2005). A similar expression pattern has been reported in the gill of Crassostrea hongkongensis under low salinity stress (Fu et al, 2011). In the current study, the high expression of HSP90-1 at 12 and 24 h was apparent in the SG (Figure 3B), but not the WG ( Figure 3A).…”

Section: Discussionsupporting

confidence: 57%

“…Bao et al ported in the hemocytes of Scylla paramamosain after high salinity stress (Yang et al, 2013). The expression of HSP90 was induced when Crassostrea hongkongensis (Fu et al, 2011) and Crassostrea gigas (Jo et al, 2008) were subject to salinity stress. In the lobster, Homarus americanus, the expression of HSP70 and HSP90 in the abdominal muscle increased after salinity stress, whereas HSP90 levels remained unchanged in the hepatopancreas (Spees et al, 2002b).…”

Section: Discussionmentioning

confidence: 99%

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mRNA expression profiles of heat shock proteins of wild and salinity-tolerant swimming crabs, Portunus trituberculatus, subjected to low salinity stress

Bao

Zhang

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Challenged by the low salinity, 4 parts per thousand (4 ppt), for 72 h, the survivals of swimming crabs (Portunus trituberculatus) were collected as the screened group (SG, tolerant to low salinity). Aiming at identifying the mechanism of low salinity tolerance, quantitative real-time PCR was employed to investigate the expression profiles of 4 HSP genes (HSP60, HSP70, HSP90-1, HSP90-2) in the hepatopancreas of wild (WG) and screened (SG) groups of P. trituberculatus exposed to low salinity (4 ppt). The results showed that 3 of the candidate genes (HSP60, HSP70, HSP90-1) exhibited similarly downregulated expression profiles in the first 3 h (P < 0.05), which became upregulated from 3 h to 72 h after being subjected to low salinity conditions. In contrast, the expression profile of the HSP90-2 gene was upregulated during the first 6 h for the WG, and during the first 12 h for the SG, after which it became downregulated. HSP90-1 and HSP90-2 were highly expressed at 12 h after low salinity challenge in the SG, but not the WG. The response of these 2 genes to salinity stress indicates their suitability as biomarkers to differentiate SG from WG crabs. The results indicate that HSP genes are involved in the adaptation of crabs to low salinity exposure, and that different HSPs have diverse functions in response to low salinity stress in P. trituberculatus. In addition, HSP expression in SG indicates that this group is more tolerant to low salinity conditions compared to WG.

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Heat Shock Proteins in Aquaculture Disease Immunology and Stress Response of Crustaceans

2017

Heat Shock Proteins

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Cloning and expression of a heat shock protein (HSP) 90 gene in the haemocytes of Crassostrea hongkongensis under osmotic stress and bacterial challenge

Cited by 88 publications

References 34 publications

Molecular cloning and expression analysis of heat shock protein 20 (HSP20) from the pearl oyster Pinctada martensii

Molecular cloning and expression analysis of heat shock protein 20 (HSP20) from the pearl oyster Pinctada martensii

mRNA expression profiles of heat shock proteins of wild and salinity-tolerant swimming crabs, Portunus trituberculatus, subjected to low salinity stress

Heat Shock Proteins in Aquaculture Disease Immunology and Stress Response of Crustaceans

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