Cloning and Expression of Activator of CREM in Testis in Human Testicular Tissue

Palermo, I.; Litrico, Laura; Emmanuele, Giovanni; Giuffrida, Vincenza; Sassone–Corsi, Paolo; Cesare, Dario De; Fimia, Gian María; D’Agata, Rosario; Calogero, Aldo E.; Travali, Salvatore

doi:10.1006/bbrc.2001.4805

Cited by 19 publications

(11 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Infertility is due to lack of expression of key postmeiotic genes i.e., protamines 1 and 2, transition proteins TNP1 and TNP2, proacrosin, and calspermin [SassoneCorsi 1998]. CREM activity in post meiotic-germ cells is enhanced by its association with LIM only protein, ACT (Activator of CREM) in the testis [Fimia et al 1999;Palermo et al 2001]. ACT is composed of four and a half LIM domains each containing two zinc finger motifs that facilitate protein-protein interactions [Fimia et al 1999], thus suggesting a role of zinc in spermatogenesis.…”

Section: Discussionmentioning

confidence: 99%

Testicular apoptosis after dietary zinc deficiency: Ultrastructural and TUNEL studies

Kumari

Nair

Bedwal

2011

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Testicular apoptosis after dietary zinc deficiency: Ultrastructural and TUNEL studies

Kumari

Nair

Bedwal

2011

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

“…ACT is a factor belonging to the class of LIM-only (LMO) proteins with a characteristic organization of four and a half LIM domains (FHL) which are structural motifs composed of two adjacent zinc fingers known to be involved in protein-protein interactions (Fimia et al, 1999;Palermo et al, 2001). ACT interacts with CREM in postmeiotic germ cells and enhances CREM-dependent transcription.…”

Section: Transcription Factorsmentioning

confidence: 99%

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 4: Intercellular bridges, mitochondria, nuclear envelope, apoptosis, ubiquitination, membrane/voltage‐gated channels, methylation/acetylation, and transcription factors

Hermo

Pelletier

Cyr

et al. 2009

Microscopy Res & Technique

View full text Add to dashboard Cite

As germ cells divide and differentiate from spermatogonia to spermatozoa, they share a number of structural and functional features that are common to all generations of germ cells and these features are discussed herein. Germ cells are linked to one another by large intercellular bridges which serve to move molecules and even large organelles from the cytoplasm of one cell to another. Mitochondria take on different shapes and features and topographical arrangements to accommodate their specific needs during spermatogenesis. The nuclear envelope and pore complex also undergo extensive modifications concomitant with the development of germ cell generations. Apoptosis is an event that is normally triggered by germ cells and involves many proteins. It occurs to limit the germ cell pool and acts as a quality control mechanism. The ubiquitin pathway comprises enzymes that ubiquitinate as well as deubiquitinate target proteins and this pathway is present and functional in germ cells. Germ cells express many proteins involved in water balance and pH control as well as voltage-gated ion channel movement. In the nucleus, proteins undergo epigenetic modifications which include methylation, acetylation, and phosphorylation, with each of these modifications signaling changes in chromatin structure. Germ cells contain specialized transcription complexes that coordinate the differentiation program of spermatogenesis, and there are many male germ cell-specific differences in the components of this machinery. All of the above features of germ cells will be discussed along with the specific proteins/genes and abnormalities to fertility related to each topic.

show abstract

“…A major difference with somatic cells is that in germ cells, CREM does not require phosphorylation at serine 117 in order to function as a transcriptional activator, nor association with CBP . Rather, CREM activity in postmeiotic germ cells is enhanced by its association with the LIM-only protein ACT (activator of CREM in the testis) , Palermo et al 2001. ACT is composed of four-and-a-half LIM domains, each containing two zinc finger motifs that facilitate protein-protein interactions .…”

Section: Regulation and Differential Activation Of Crem In Germ Cellsmentioning

confidence: 99%

“…Most LIM proteins have been determined to have roles in cell differentiation and development by influencing either transcriptional or cytoskeletal components. During spermatogenesis in mice and humans the expression of CREM and ACT is spatially and temporally paired , Palermo et al 2001, and together they function as powerful transcriptional activators. A further role for zinc-finger-containing proteins in transcription is indicated by the discovery of testicular zinc finger protein (TZF) and its splice variant, TZF-L. Interestingly, the gene for these proteins contains CREs in the promoter and distribution is restricted to the nucleus of pachytene-stage cells, suggesting they may have a role in gene transcription related to formation of the synaptonemal complex (Ishizuka et al 2003).…”

Section: Regulation and Differential Activation Of Crem In Germ Cellsmentioning

confidence: 99%

Testis-specific transcription mechanisms promoting male germ-cell differentiation

Kimmins¹,

Kotaja²,

Davidson³

et al. 2004

Reproduction

Self Cite

147

View full text Add to dashboard Cite

Male germ-cell differentiation requires spermatogenic stage-and cell-specific gene expression that is achieved by unique chromatin remodeling, transcriptional control and the expression of testis-specific genes or isoforms. Recent findings have shown that the testis has specialized transcription complexes that coordinate the differentiation program of spermatogenesis. There are male germ cell-specific differences in the components of the general transcription machinery. These include upregulated expression of the TATA-binding protein (TBP) family and its associated cofactors. Importantly, a member of the TBP family, TBP-like factor (TLF), has a distribution pattern that is dependent on the spermatogenic cycle and is essential for spermatogenesis. Interestingly TBP-associated factor (TAF7), a factor of the transcription factor (TF)IID complex, is exchanged at a critical stage in germ cell development for the testis-specific paralogue TAF7L. A compelling amount of data has established that cAMP-response-element modulator (CREM), a transcription factor responsive to the cAMP signal transduction pathway, drives expression of key testis-specific genes. In this review we summarize recent advances in the transcription machinery that is testis-specific, gene-selective and necessary for the process of spermatogenesis.

show abstract

Cloning and Expression of Activator of CREM in Testis in Human Testicular Tissue

Cited by 19 publications

References 19 publications

Testicular apoptosis after dietary zinc deficiency: Ultrastructural and TUNEL studies

Testicular apoptosis after dietary zinc deficiency: Ultrastructural and TUNEL studies

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 4: Intercellular bridges, mitochondria, nuclear envelope, apoptosis, ubiquitination, membrane/voltage‐gated channels, methylation/acetylation, and transcription factors

Testis-specific transcription mechanisms promoting male germ-cell differentiation

Contact Info

Product

Resources

About