Cloning and Expression of an Adhesin Antigen of Streptococcus sanguis G9B in Escherichia coli

Rosan, Burton; Baker, Carol T.; Nelson, Genevieve M.; Berman, Richard; Lamont, Richard J.; Demuth, Donald R.

doi:10.1099/00221287-135-3-531

Cited by 20 publications

(15 citation statements)

References 26 publications

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“…A gene with similar function to ssp5 has been cloned from 5. sanguis and designated adh. 106 This gene was expressed as a (3-galactosidase fusion protein in which the cloned streptococcal gene product was estimated to be 84 kDa. This product crossreacted with antisera to the purified 80 kDa surface protein antigen of S. sanguis that is involved in the adherence of this organism to saliva-coated hydroxyapatite.…”

Section: Adhesin Proteinsmentioning

confidence: 99%

Genetic Approaches to The Study of Oral Microflora: A Review

Macrina

Dertzbaugh

Halula

et al. 1990

Critical Reviews in Oral Biology & Medicine

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As the study of oral microorganisms intensified almost 2 decades ago, the application of genetic techniques resulted in important contributions to the understanding of this clinically and ecologically important group of bacteria. The isolation and characterization of mutants of cariogenic streptococci helped to focus attention on traits that were important in colonization and virulence. Such classic genetic approaches gave way to molecular genetic techniques, including recombinant DNA methodology in the late 1970s. Gene cloning systems and methods to move DNA into cells have been developed for oral streptococci. Many streptococcal genes thought to be important in colonization and virulence have since been cloned and their nucleotide sequence determined. Mutant strains have been constructed using defective copies of cloned genes in order to create specific genetic lesions on the bacterial chromosome. By testing such mutants in animal models, a picture of the cellular and molecular basis of dental caries is beginning to emerge. These modern genetic methodologies also are being employed to develop novel and efficacious cell-free or whole cell vaccines against this infection. Genetic approaches and analyses are now being used to dissect microorganisms important in periodontal disease as well. Such systems should be able to exploit advances made in genetically manipulating related anaerobes, such as the intestinal Bacteroides. Gene cloning techniques in oral anaerobes, Actinomyces and Actinobacillus, are already beginning to pay dividends in helping understand gene structure and expression. Additional effort is needed to develop facile systems for genetic manipulation of these important groups of microorganisms.

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Section: Adhesin Proteinsmentioning

confidence: 99%

Genetic Approaches to The Study of Oral Microflora: A Review

Macrina

Dertzbaugh

Halula

et al. 1990

Critical Reviews in Oral Biology & Medicine

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“…Although numerous studies have elucidated the mechanisms of initial streptococcal adhesion (7,13,17,22,25,31,51,52), the subsequent process of bacterial accumulation and proliferation leading to functionally heterogeneously organized sessile communities called dental plaque is poorly understood.…”

mentioning

confidence: 99%

Streptococcus gordonii Biofilm Formation: Identification of Genes that Code for Biofilm Phenotypes

Loo¹,

Corliss²,

Ganeshkumar³

2000

J Bacteriol

457

420

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Viridans streptococci, which include Streptococcus gordonii, are pioneer oral bacteria that initiate dental plaque formation. Sessile bacteria in a biofilm exhibit a mode of growth that is distinct from that of planktonic bacteria. Biofilm formation of S. gordonii Challis was characterized using an in vitro biofilm formation assay on polystyrene surfaces. The same assay was used as a nonbiased method to screen isogenic mutants generated by Tn916 transposon mutagenesis for defective biofilm formation. Biofilms formed optimally when bacteria were grown in a minimal medium under anaerobic conditions. Biofilm formation was affected by changes in pH, osmolarity, and carbohydrate content of the growth media. Eighteen biofilm-defective mutants of S. gordonii Challis were identified based on Southern hybridization with a Tn916-based probe and DNA sequences of the Tn916-flanking regions. Molecular analyses of these mutants showed that some of the genes required for biofilm formation are involved in signal transduction, peptidoglycan biosynthesis, and adhesion. These characteristics are associated with quorum sensing, osmoadaptation, and adhesion functions in oral streptococci. Only nine of the biofilm-defective mutants had defects in genes of known function, suggesting that novel aspects of bacterial physiology may play a part in biofilm formation. Further identification and characterization of biofilmassociated genes will provide insight into the molecular mechanisms of biofilm formation of oral streptococci.

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“…Adhesins are continuing to be isolated, characterized, and the adhesin genes cloned, from a variety of prominent, and less proportionally prominent, indigenous plaque bacteria. Adhesins from mutans streptococci, sanguis streptococci, Actinomyces, Haemophilus, Neisseria, Fusobacteria, Eikenella, Pre6otella, Porphyromonas and others have been described (48)(49)(50)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61)(62)(63)(64)(65)(66).…”

Section: Microbial Adhesinsmentioning

confidence: 99%

Microbial Ecology of Marginal Gingivitis

Liljemark

2000

Microbial Ecology in Health and Disease

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Cloning and Expression of an Adhesin Antigen of Streptococcus sanguis G9B in Escherichia coli

Cited by 20 publications

References 26 publications

Genetic Approaches to The Study of Oral Microflora: A Review

Genetic Approaches to The Study of Oral Microflora: A Review

Streptococcus gordonii Biofilm Formation: Identification of Genes that Code for Biofilm Phenotypes

Microbial Ecology of Marginal Gingivitis

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