Cloning and expression of ethylene receptor ERS1 at various developmental and ripening stages of mango fruit

Abstract

ABSTRACT. Ethylene induces characteristic ripening reactions in climacteric fruits through its binding to histidine-kinase (HK) receptors, activating the expression of ripening genes. Ethylene receptors have been found in Arabidopsis thaliana (Brassicaceae) and some fruits; number and expression patterns differ among species. In mango, only ethylene receptor ETR1 was known. We cloned ERS1 cDNA from mango, and evaluated the expression of Mi-ERS1 and Mi-ETR1 by qPCR in developmental and ripening stages of this fruit. The Mi-ERS1 coding sequence is 1890 bp long and encodes 629 amino acids, similar to ERS1 from other fruits. Also, the amino acid sequence of ERS1 C-terminal HK domain shows the cognate fold after molecular modeling. Mi-ERS1 expression levels increased as mangoes ripened, showing the highest levels at the climacteric stage, while Mi-ETR1 levels did not change during development and ripening. We conclude that the patterns of expression of Mi-ERS1 and Mi-ETR1 differ in mango fruit.

The Role of 1‐Methylcyclopropene in the regulation of ethylene biosynthesis and ethylene receptor gene expression in Mangifera indica L. (Mango Fruit)

“…Loss of the response regulator domain of ERS1 was also identified in ERS2 among five members, ETR1, ERS1, ETR2, ERS2, and EIN4 of ethylene receptor family (Shakeel et al, ). The response regulator domain has been shown to be involved in the transfer of phosphate in other well‐characterized signal pathways (Contreras‐Vergara et al, ; Pang et al, ; Rasori et al, ). Hence, this difference in structure may affect signaling to downstream effectors.…”

“…At the N-terminus, the clone also includes three hydrophobic regions, with features of ETR homologs ( Figure 5). The MiETR1 and MiERS1 genes contain a histidine kinase domain and a sensor domain, playing crucial roles in the normal function of ethylene receptors and ETR-or ERS-type proteins (Bleecker, 1999 shown to be involved in the transfer of phosphate in other well-characterized signal pathways (Contreras-Vergara et al, 2012;Pang et al, 2007;Rasori et al, 2002). Hence, this difference in structure may affect signaling to downstream effectors.…”