Cloning and Expression of Fasciola gigantica Cathepsin-B Recombinant Proteins

Abstract

Fasciola gigantica cathepsin (cysteine) proteases are potential diagnostic antigens for animal and human fasciolosis. These include cathepsin-L proteases that have been exploited in the diagnosis of animal fasciolosis. However, no scientific data on the diagnostic potential of F. gigantica cathepsin B proteases is available. Therefore, three recombinant antigens of F. gigantica viz. cathepsin (cat) B-1, cat B-2 and cat B-3 were expressed in prokaryotic expression system. The recombinant antigens were purified under denaturing conditions by Nickel affinity chromatography and an optimal level of the recombinant proteins was obtained. These recombinant proteins will further be evaluated for their potential in the early prepatent diagnosis of F. gigantica infection in domestic ruminants.

Development of a colloidal gold immunochromatographic strip for the rapid detection of antibodies against Fasciola gigantica in buffalo

“…Given that the antigenantibody binding of ICS should be completed in a relatively short time (typically within 10 min), this method necessitates higher sensitivity of diagnostic antigens. For F. gigantica, the indirect ELISA was established based on several antigens (9)(10)(11)(12)23), including FgESP, rFgCatL1, FgF22, and rFgSAP-2. Among them, FgESP and FgF22 showed no cross-reactivity with cattle-derived Paramphistomum epiclitum-positive serum and buffalo-derived B. bovis-positive serum, indicating its low cross-reactivity.…”